本文關(guān)鍵詞：嗎啡戒斷對(duì)小鼠自發(fā)活動(dòng)及場(chǎng)景性恐懼條件化的影響　出處：《南華大學(xué)》2007年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 嗎啡 戒斷 自發(fā)活動(dòng) 僵直 恐懼條件化 小鼠

【摘要】： 目的:1.本研究擬通過檢測(cè)嗎啡戒斷對(duì)小鼠自發(fā)活動(dòng)的影響以期驗(yàn)證曠場(chǎng)實(shí)驗(yàn)作為研究嗎啡戒斷伴焦慮樣行為動(dòng)物模型的有效性。2.檢測(cè)嗎啡戒斷對(duì)小鼠場(chǎng)景性恐懼條件化的影響。 方法:以劑量遞增法連續(xù)皮下注射(sc)鹽酸嗎啡7d建立嗎啡依賴小鼠模型。動(dòng)物隨機(jī)分為嗎啡戒斷1d組及其生理鹽水對(duì)照組、嗎啡戒斷7d組及其生理鹽水對(duì)照組、嗎啡戒斷21d組及其生理鹽水對(duì)照組。各組動(dòng)物在相應(yīng)時(shí)間點(diǎn)采用曠場(chǎng)實(shí)驗(yàn)對(duì)自發(fā)活動(dòng)(穿梭格數(shù)、直立次數(shù)、理毛時(shí)間、進(jìn)入中央次數(shù)、中央?yún)^(qū)活動(dòng)時(shí)間及外周區(qū)活動(dòng)時(shí)間等指標(biāo))進(jìn)行檢測(cè);隨后進(jìn)行場(chǎng)景性恐懼條件化訓(xùn)練,并與24h后進(jìn)行條件化恐懼反應(yīng)(僵直)檢測(cè)。 結(jié)果:嗎啡戒斷1d組小鼠的穿梭格數(shù)、直立次數(shù)、進(jìn)入中央?yún)^(qū)次數(shù)及中央?yún)^(qū)活動(dòng)時(shí)間對(duì)比對(duì)照組均顯著降低(p0.001,p0.001,p0.001,p0.001),而理毛時(shí)間和外周區(qū)活動(dòng)時(shí)間與其對(duì)照組相比顯著增加(p0.05,p0.01)。嗎啡戒斷7d組小鼠的穿梭格數(shù)、進(jìn)入中央?yún)^(qū)次數(shù)及中央?yún)^(qū)活動(dòng)時(shí)間與對(duì)照組比較顯著降低(p0.05,p0.05,p0.05),同時(shí)外周區(qū)活動(dòng)時(shí)間顯著增多(p0.05),但是直立次數(shù)及理毛時(shí)間與其對(duì)照組比較沒有顯著性差異(p㧐0.05, p㧐0.05)。嗎啡戒斷21d組小鼠各項(xiàng)檢測(cè)指標(biāo)與其對(duì)照組比較差異均無顯著性(p㧐0.05, p㧐0.05, p㧐0.05, p㧐0.05, p㧐0.05, p㧐0.05)。另外,與生理鹽水對(duì)照組比較,嗎啡戒斷1d組小鼠的僵直百分比顯著下降(p0.05),而嗎啡戒斷7d組和嗎啡戒斷21d組小鼠的僵直百分比與各自對(duì)照組比較無顯著差異(p㧐0.05, p㧐0.05)。 結(jié)論:1.嗎啡依賴小鼠在戒斷早期其主要自發(fā)活動(dòng)指標(biāo)均發(fā)生顯著性改變;隨著戒斷期延續(xù),其自發(fā)活動(dòng)逐漸恢復(fù)正常。提示曠場(chǎng)實(shí)驗(yàn)可以作為研究嗎啡戒斷早期伴焦慮樣行為的有效動(dòng)物模型。2.嗎啡戒斷可時(shí)間依賴性損傷小鼠場(chǎng)景性恐懼條件化。
[Abstract]:Objective: 1., the purpose of this study is to detect the effect of morphine withdrawal on spontaneous activity in mice, so as to verify the validity of open field test as a model for morphine withdrawal and anxiety like behavior..2. was used to detect the effect of morphine withdrawal on the conditioning of scene fear in mice.
Methods: with the increasing dosage by subcutaneous injection of morphine hydrochloride (SC) 7d in morphine dependent mice. Animal model were divided into 1D group and morphine withdrawal saline control group, morphine withdrawal group 7d and normal saline control group, morphine withdrawal group 21d and saline control group. Each animal by open field experiment on spontaneous activity in the corresponding time points (shuttle cell number, vertical frequency, grooming time, enter the central frequency, central activity time and the peripheral zone time index) were detected; then the contextual fear conditioning training, and the conditioned fear response and 24h (stiff) detection.
Results: the number of shuttle, lattice of morphine withdrawal mice in 1D group rearing, in contrast the central frequency and central time control group decreased significantly (p0.001, p0.001, p0.001, p0.001), and the hair was significantly increased compared with time and peripheral activity time and the control group (P0.05, P0.01). The number of shuttle lattice morphine withdrawal of mice in the 7d group, entered the central frequency and central time compared with the control group decreased significantly (P0.05, P0.05, P0.05), and peripheral zone activities increased significantly (P0.05), but the rearing and grooming time compared with the control group had no significant difference (P? 0.05, p? 0.05) there were no significant differences. Compared with morphine withdrawal mice in group 21d of the indexes and the control group (P? 0.05, P? 0.05, P? 0.05, P? 0.05, P? 0.05, P? 0.05). In addition, compared with saline control group, the percentage of stiff morphine withdrawal mice in group 1D decreased significantly (P 0.05) there was no significant difference in the percentage of rats in the morphine withdrawal group 7d and the morphine abstinence 21d group (P? 0.05, P? 0.05).
Conclusion: in the early withdrawal of the main indicators of spontaneous activity changes of 1. morphine dependent mice; with the withdrawal period of continuation of the spontaneous activity gradually returned to normal. That open field test can be used as.2. effective animal model of morphine withdrawal early with anxiety like behavior of morphine withdrawal time dependent contextual fear conditioning injury in mice.

【學(xué)位授予單位】：南華大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2007
【分類號(hào)】：R363

【共引文獻(xiàn)】

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1 劉小粉;顏?zhàn)恿?王建紅;胡新天;馬原野;;長(zhǎng)期注射嗎啡對(duì)戒斷后小鼠Y-迷宮空間識(shí)別記憶的影響[J];動(dòng)物學(xué)研究;2009年02期

2 李竹,吳春福;阿片系統(tǒng)與學(xué)習(xí)記憶[J];沈陽藥科大學(xué)學(xué)報(bào);2000年05期

3 Yan Yu;Changchun Zeng;Siyun Shu;Xuemei Liu;Chuhua Li;;Similar effects of substance P on learning and memory function between hippocampus and striatal marginal division[J];Neural Regeneration Research;2014年08期

4 馬光瑜,樊小力,徐小虎,韓太真;嗎啡成癮對(duì)大鼠及小鼠學(xué)習(xí)與記憶能力影響的實(shí)驗(yàn)研究[J];中國(guó)行為醫(yī)學(xué)科學(xué);2000年05期

5 ;Repeated morphine treatment influences operant and spatial learning differentially[J];Neuroscience Bulletin;2006年03期

6 宋贈(zèng);田紹文;;可卡因戒斷后覓藥行為的潛伏及其分子學(xué)機(jī)制[J];中國(guó)藥物濫用防治雜志;2007年03期

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2 王緒軼;海洛因成癮者停止用藥后不同時(shí)間腦功能和結(jié)構(gòu)的研究[D];中南大學(xué);2006年

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4 龔應(yīng)霞;中樞組胺對(duì)嗎啡成癮的形成和戒斷后恐懼記憶作用[D];浙江大學(xué);2008年

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1 李百瑋;海洛因成癮大鼠腦NO、MDA、SOD和血腦屏障通透性的變化[D];廣西醫(yī)科大學(xué);2011年

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5 閻楠;大連市初中生網(wǎng)絡(luò)成癮者應(yīng)激中間變量特點(diǎn)研究[D];大連醫(yī)科大學(xué);2008年

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本文編號(hào)：1414560