發(fā)布時(shí)間：2018-01-12 09:32

  本文關(guān)鍵詞：大鼠脂肪干細(xì)胞誘導(dǎo)為成骨細(xì)胞三維立體培養(yǎng)的實(shí)驗(yàn)研究　出處：《山東大學(xué)》2006年碩士論文　論文類(lèi)型：學(xué)位論文

  更多相關(guān)文章： 脂肪基質(zhì)干細(xì)胞 成骨誘導(dǎo) 殼聚糖 磷酸三鈣 三維培養(yǎng) 骨組織工程 支架材料

【摘要】：研究背景 因創(chuàng)傷、腫瘤、感染等各種原因造成的牙槽骨及頜骨缺損、缺失臨床上十分常見(jiàn)，目前常用的修復(fù)方法有自體骨移植、異體或異種骨移植、人工合成代用品等，但這些方法都存在著各自的不足，如供體來(lái)源不足、免疫排斥反應(yīng)等，不能完全滿足臨床需要。隨著細(xì)胞生物學(xué)的迅猛發(fā)展和組織工程理論的日臻完善，組織工程技術(shù)為頜骨缺損修復(fù)開(kāi)辟了全新的途徑。 骨組織工程的基本原理是：取少量的種子細(xì)胞，將其體外擴(kuò)增達(dá)到需要的數(shù)量后種植于天然的或人工合成的具有良好生物相容性和生物降解性的細(xì)胞外基質(zhì)材料上，經(jīng)過(guò)三維立體培養(yǎng)，形成細(xì)胞生物材料復(fù)合物后回植體內(nèi)，支架材料上的細(xì)胞繼續(xù)生長(zhǎng)、增殖，分泌細(xì)胞外基質(zhì)(ECM)形成骨組織，而支架材料逐步降解，達(dá)到修復(fù)骨缺損和重建功能的目的。骨組織工程由種子細(xì)胞，，支架材料(ECM)，細(xì)胞／支架復(fù)合體的構(gòu)建三個(gè)關(guān)鍵因素組成。 選擇來(lái)源豐富、容易擴(kuò)增的理想的種子細(xì)胞是骨組織工程研究的重點(diǎn)及前提。目前組織工程種子細(xì)胞的研究主要集中在胚胎干細(xì)胞(embryonic stem cells，ESC)和成體干細(xì)胞(adult stem cells，ASC)。胚胎干細(xì)胞存在著倫理、法律及免疫排斥反應(yīng)等問(wèn)題，目前難以用于臨床。骨髓基質(zhì)干細(xì)胞(bone marrow stromal cells，BMSCs)是至今研究得最為廣泛的成體干細(xì)胞之一。體內(nèi)、體外實(shí)驗(yàn)均證明，BMSCs具有多向分化潛能，在不同的誘導(dǎo)環(huán)境下能向成骨細(xì)胞、脂肪細(xì)胞、軟骨細(xì)胞、肌肉細(xì)胞、神經(jīng)細(xì)胞及心肌細(xì)胞等定向分化；BMSCs在體外擴(kuò)
[Abstract]:Research background Alveolar bone and jaw defects caused by trauma, tumor, infection and other reasons are very common in clinic. At present, autogenous bone transplantation, allogeneic or allogeneic bone transplantation are commonly used to repair the defects. Synthetic substitutes and so on, but these methods have their own shortcomings, such as insufficient donor sources, immune rejection and so on. With the rapid development of cell biology and the improvement of tissue engineering theory, tissue engineering technology has opened up a new way for the repair of jaw defects. The basic principle of bone tissue engineering is to take a small number of seed cells. After amplification in vitro to the required amount, it was planted on natural or synthetic extracellular matrix materials with good biocompatibility and biodegradability. After the formation of cellular biomaterial complex, the cells on the scaffold material continued to grow, proliferate, secrete extracellular matrix ECM) to form bone tissue, and the scaffold material gradually degraded. Bone tissue engineering consists of three key factors: seed cells, scaffold material ECM, and cell / scaffold complex construction. There are plenty of sources of choice. The ideal seed cells that can be amplified easily are the focus and premise of bone tissue engineering. At present, the research of seed cells in tissue engineering is mainly focused on embryonic stem cells (. Embryonic stem cells. ESCs and adult stem cells. There are ethical, legal and immune rejection problems in embryonic stem cells. At present, it is difficult to use in clinic. Bone marrow stromal cells. BMSCs is one of the most widely studied adult stem cells. In vivo and in vitro experiments have proved that BMSCs have the potential to differentiate into osteoblasts in different induced environments. Adipocytes, chondrocytes, muscle cells, nerve cells and cardiomyocytes were differentiated. BMSCs expansion in vitro
【學(xué)位授予單位】：山東大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2006
【分類(lèi)號(hào)】：R329.2

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