本文關(guān)鍵詞：利用抑制性削減雜交技術(shù)研究抗CD3、CD28單抗共刺激人T淋巴細(xì)胞的基因差異表達(dá)　出處：《蘇州大學(xué)》2006年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 抑制性削減雜交 T細(xì)胞 信號轉(zhuǎn)導(dǎo) 抗人CD3單抗 抗人CD28單抗

【摘要】： 通過TCR產(chǎn)生的第一信號和B7與CD28結(jié)合所介導(dǎo)的第二信號,導(dǎo)致人初始T細(xì)胞的活化、增殖與免疫反應(yīng)。這些信號受一個(gè)復(fù)雜的網(wǎng)絡(luò)調(diào)節(jié),該網(wǎng)絡(luò)是由許多基因組成和參與的。為了研究參與人T細(xì)胞信號轉(zhuǎn)導(dǎo)的基因,本文以正常人初始T細(xì)胞作為對照組,以抗人CD3單抗聯(lián)合抗人CD28單抗激發(fā)的人T細(xì)胞作為實(shí)驗(yàn)組,進(jìn)行抑制性削減雜交。結(jié)果篩選到43個(gè)侯選克隆,對其單向測序后,經(jīng)過與GenBank同源序列比較,證實(shí)了4個(gè)克隆所編碼的基因參與了T細(xì)胞信號轉(zhuǎn)導(dǎo)的下游通路,分別為CCND2、RHOF、RHOA和IRF4;新發(fā)現(xiàn)19個(gè)克隆所代表的基因(其中4個(gè)為重復(fù)克隆)可能與T細(xì)胞信號轉(zhuǎn)導(dǎo)途徑有關(guān);另外20個(gè)克隆在GenBank中無法查到對應(yīng)的同源基因,可能代表了新基因。對這些基因的進(jìn)一步研究,有助于闡釋T細(xì)胞的信號轉(zhuǎn)導(dǎo)機(jī)制,為了解體內(nèi)活化的T細(xì)胞參與的生理和病理過程,具有重要的指導(dǎo)意義。
[Abstract]:The first signal produced by TCR and the second signal mediated by the binding of B7 to CD28 lead to the activation, proliferation and immune response of human initial T cells. These signals are regulated by a complex network. This network is composed of many genes. In order to study the genes involved in T cell signal transduction, we use the normal human initial T cells as the control group. Human T cells stimulated by anti-human CD3 monoclonal antibody and anti-human CD28 monoclonal antibody were used as experimental group to perform suppression subtractive hybridization. Results 43 candidate clones were screened and sequenced. Compared with the homologous sequence of GenBank, it was confirmed that the genes encoded by the four clones were involved in the downstream pathway of T cell signal transduction, namely CCND2RHOFFHOA and IRF4, respectively. The genes represented by 19 clones (4 of which were repeated clones) may be related to the signal transduction pathway of T cells. The other 20 clones could not find the corresponding homologous genes in GenBank, which may represent new genes. Further study of these genes will help to explain the signal transduction mechanism of T cells. In order to understand the physiological and pathological process of activated T cells in vivo, it has important guiding significance.
【學(xué)位授予單位】：蘇州大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2006
【分類號】：R392;Q789

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