本文關(guān)鍵詞：肺炎衣原體AR39菌株中參與泛醌合成的酶UbiD和UbiG的鑒定　出處：《上海交通大學》2006年博士論文　論文類型：學位論文

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【摘要】： 衣原體是與革蘭氏陰性菌有密切關(guān)系的在真核細胞內(nèi)嚴格寄生的原核微生物,其生活周期分為原體(EB)和始體(RB)兩個階段。在原體時期,肺炎衣原體沒有明顯的代謝活動,但是其衣原體外膜能夠侵染宿主細胞,從而分化成為始體。在始體時期,衣原體完成生長和繁殖過程。衣原體在感染宿主細胞后的二十多個小時內(nèi),以二分裂的方式產(chǎn)生大量的子代原體。子代原體成熟后通過裂解細胞的方式釋放,從而完成一個生活周期。肺炎衣原體通過侵染人呼吸系統(tǒng)的上皮細胞,從而引起急性或慢性非典型性肺炎。此外研究還表明,肺炎衣原體可以引起動脈粥樣硬化等多種心血管疾病。 由于肺炎衣原體獨特的生活周期,人們對其相關(guān)的生物化學和分子生物學的研究都較少,已有的研究主要集中于疾病的診斷和治療方面。相比之下,對其內(nèi)部機制的探究還不夠充分。2000年肺炎衣原體AR39菌株的基因組測序工作完成,在基因組的1127個編碼蛋白中,功能未知的蛋白質(zhì)為531個,占全部編碼蛋白的47.1%。對這些功能未知的蛋白質(zhì)的研究,將有助于人們了解肺炎衣原體獨殊的生活方式及相應的致病機理�；蚪M序列分析表明,肺炎衣原體具有很多參與芳香類化合物合成及代謝的酶,如合成酶、羥化酶、脫羧酶和甲基化酶。芳香類化合物及其衍生物,如泛醌、苯丙氨酸等,在生物體內(nèi)具有十分重要的作用。衣原體蛋白組學的研究表明,參與芳香類化合物合成及代謝的酶在原體時期均不表達,因此推斷這些酶應該是在始體時期發(fā)揮作用,即在衣原體的生長及繁殖時期發(fā)揮功能。對于芳香類化合物及其衍生物的合成及代謝途徑的研究,可為進一步了解肺炎衣原體提供幫助。 利用生物學軟件分析肺炎衣原體的基因組,發(fā)現(xiàn)一個功能未知的蛋白質(zhì)
[Abstract]:Chlamydia is a strictly parasitic prokaryotic microorganism in eukaryotic cells closely related to gram-negative bacteria. The life cycle of chlamydia is divided into two stages, I. e. Chlamydia pneumoniae has no obvious metabolic activity, but the outer membrane of chlamydia pneumoniae can infect host cells and differentiate into estrus. Chlamydia complete growth and reproduction. Chlamydia in the host cells in more than 20 hours after infection, in a two-fission way to produce a large number of progeny. The offspring mature through the release of lytic cells. Chlamydia pneumoniae infects epithelial cells of the human respiratory system, causing acute or chronic atypical pneumonia. Chlamydia pneumoniae can cause atherosclerosis and other cardiovascular diseases. Due to the unique life cycle of Chlamydia pneumoniae, there are few studies on its biochemistry and molecular biology. In 2000, the genome sequencing of Chlamydia pneumoniae AR39 strain was completed, in the genome of 1127 coding proteins. The number of unknown proteins was 531, accounting for 47.1% of the total proteins. Genomic sequence analysis shows that Chlamydia pneumoniae has many enzymes, such as synthase, involved in the synthesis and metabolism of aromatic compounds. Hydroxylase, decarboxylase and methylase. Aromatic compounds and their derivatives, such as ubiquinone, phenylalanine, play a very important role in organisms. The enzymes involved in the synthesis and metabolism of aromatic compounds are not expressed in the mycoplasma stage, so it is concluded that these enzymes may play a role in the initiation stage. The study of synthesis and metabolic pathway of aromatic compounds and its derivatives may be helpful for further understanding of chlamydia pneumoniae. The genome of Chlamydia pneumoniae was analyzed by biological software and a protein with unknown function was found.
【學位授予單位】：上海交通大學
【學位級別】：博士
【學位授予年份】：2006
【分類號】：R374

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