本文關(guān)鍵詞：人類心臟發(fā)育候選基因ZNF569的研究　出處：《湖南師范大學(xué)》2005年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 心臟發(fā)育 ZNF569 鋅指蛋白 MAPK 轉(zhuǎn)錄因子

【摘要】：心血管系統(tǒng)疾病已經(jīng)成為威脅人類健康的最大的兩個(gè)敵人之一。在心血管系統(tǒng)疾病的研究中已發(fā)現(xiàn),某些基因在時(shí)空上的表達(dá)紊亂可導(dǎo)致先天性心臟病,而對(duì)部分心血管系統(tǒng)病癥高發(fā)人群的研究也證明部分后天形成的心臟病與遺傳因素有關(guān)。我們研究室進(jìn)行的大規(guī)模篩選克隆人類心臟發(fā)育的相關(guān)基因工作為理解心臟發(fā)育的機(jī)理、探索心臟發(fā)育中的分子調(diào)控機(jī)制打下了堅(jiān)實(shí)的基礎(chǔ)。 ZNF569就是本文作者在利用生物信息學(xué)方法篩選人類心臟發(fā)育候選基因的過程中發(fā)現(xiàn)的一個(gè)人類新基因。ZNF569 cDNA全長(zhǎng)4071個(gè)堿基,ORF全長(zhǎng)2061個(gè)堿基,編碼一個(gè)長(zhǎng)686個(gè)氨基酸殘基的蛋白質(zhì)。該蛋白包含一個(gè)KRAB結(jié)構(gòu)域和18個(gè)鋅指結(jié)構(gòu)域,在包括小鼠、大鼠、果蠅在內(nèi)的多個(gè)物種中均發(fā)現(xiàn)了它的同源蛋白,并且它們之間具有極高的相似度,說明此蛋白所在的亞家族可能具有相當(dāng)重要的功能。Northern結(jié)果顯示ZNF569在成人的肝、心臟和胎盤中有高表達(dá),在胰腺和骨骼肌中表達(dá)較弱,在胚胎心臟、骨骼肌和肝也有表達(dá),提示該基因可能與心臟發(fā)育有關(guān)。轉(zhuǎn)錄活性分析表明在COS-7細(xì)胞中過表達(dá)ZNF569蛋白可以使MAPK信號(hào)途徑的兩個(gè)下游轉(zhuǎn)錄因子AP-1和SRE的轉(zhuǎn)錄抑制活性顯著下降。ZNF569在核質(zhì)內(nèi)的亞細(xì)胞定位分析進(jìn)一步證明了其作為轉(zhuǎn)錄因子的作用。對(duì)ZNF569的分段report assays分析表明,ZNF569可能通過KRAB框和C2H2鋅指結(jié)構(gòu)域行使其抑制轉(zhuǎn)錄的作用。綜合目前的研究結(jié)果來看,ZNF569可以通過MAPK信號(hào)途徑參與了對(duì)細(xì)胞生命活動(dòng)的調(diào)控過程。 本文作者還對(duì)一個(gè)Rho family GTPases交換因子hGEFT進(jìn)行了
[Abstract]:Cardiovascular disease has become one of the two greatest threats to human health. In the study of cardiovascular diseases, it has been found that the disorder of the expression of some genes in time and space can lead to congenital heart disease. The study of some people with high incidence of cardiovascular diseases also proved that some acquired heart disease is related to genetic factors. Our lab carried out large-scale screening to clone human heart development related genes to understand. The mechanism of heart development. Exploring the molecular regulation mechanism of heart development has laid a solid foundation. ZNF569 is a new human gene. ZNF569, which the author found in the process of screening candidate genes for human heart development using bioinformatics. The length of cDNA was 4071 bases. ORF is 2061 bases in length and encodes a 686 amino acid residues. The protein contains a KRAB domain and 18 zinc finger domains in mice and rats. Its homologous proteins have been found in many species including Drosophila, and there is a high similarity between them. Northern results showed that ZNF569 was highly expressed in adult liver, heart and placenta. The expression was weak in pancreas and skeletal muscle, but also in fetal heart, skeletal muscle and liver. The transcriptional activity analysis showed that overexpression of ZNF569 protein in COS-7 cells could cause two downstream transcription factors AP-1 and SR of MAPK signaling pathway. The subcellular localization analysis of ZNF569 in nuclear and cytoplasm further demonstrated its role as a transcription factor. Segmented report for ZNF569. Assays analysis showed that. ZNF569 may play a role in inhibiting transcription through KRAB frame and C2H2 zinc finger domain. ZNF569 may participate in the regulation of cell life through MAPK signaling pathway. The author also describes a Rho family GTPases exchange factor, hGEFT.
【學(xué)位授予單位】：湖南師范大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2005
【分類號(hào)】：R346

【共引文獻(xiàn)】

相關(guān)期刊論文 前4條

1 張艷君;梁淑媛;王躍群;吳秀山;;pCMV-BD-ZNF424和pCMV-Tag2-ZNF424缺失突變重組質(zhì)粒的構(gòu)建[J];生命科學(xué)研究;2006年S2期

2 鄧志偉;胡君健;吳秀山;袁婺洲;;果蠅新基因CG7609的克隆與初步功能研究[J];生命科學(xué)研究;2009年05期

3 張艷陽;唐e，

本文編號(hào)：1381942