本文關(guān)鍵詞：微導(dǎo)管法建立大腦中動(dòng)脈閉塞性兔腦缺血—再灌注模型　出處：《延邊大學(xué)》2007年碩士論文　論文類型：學(xué)位論文

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【摘要】： 目的探討建立穩(wěn)定性、可靠性、可重復(fù)性強(qiáng)的動(dòng)物腦缺血模型的方法。 方法日本大耳白兔50只，雌雄不限，隨機(jī)分為A，B，C三組。其中A組10只，為假手術(shù)對(duì)照組：B組20只，為微導(dǎo)管栓塞組：C組20只，為線栓組。各組動(dòng)物麻醉后，暴露一側(cè)CCA、ECA、ICA，A組只暴露動(dòng)脈不予處理，B組以直徑為0.50mm的硬膜外導(dǎo)管經(jīng)CCA、ICA置入MCA，C組以相同直徑的線栓經(jīng)相同路徑置入MCA。MCAO判定方法為，B組采用經(jīng)特殊拉伸制作的硬膜外導(dǎo)管接醫(yī)用壓力換能器及測(cè)壓裝置用以監(jiān)測(cè)動(dòng)脈壓力波形，，以導(dǎo)管插入達(dá)預(yù)定深度且動(dòng)脈壓力波形消失作為MCA被成功阻閉的判定標(biāo)準(zhǔn)：C組則按文獻(xiàn)根據(jù)線栓置入長度作為判定標(biāo)準(zhǔn)。兩組動(dòng)物均于MCAO一小時(shí)后恢復(fù)再灌注，并于24小時(shí)后觀察神經(jīng)學(xué)行為改變情況，依照PHILIP評(píng)分法進(jìn)行評(píng)分后，(?)頭取腦。行TTC及HE染色評(píng)價(jià)模型效果。結(jié)果：B、C兩組模型經(jīng)MCAO后24小時(shí)行PHILIP評(píng)分及TTC染色及HE染色電鏡觀察均證實(shí)梗死灶存在。成功率：B組為75％，C組為65％。兩組比較無顯著差異(P＞0.05)。結(jié)論：微導(dǎo)管法以壓力換能器監(jiān)測(cè)動(dòng)脈波形作為準(zhǔn)確判定MCAO成功標(biāo)準(zhǔn)，效果直觀可靠易于操作，是一種理想的制作兔腦缺血-再灌注模型的方法。
[Abstract]:Objective to explore the method of establishing a stable, reliable and reproducible animal model of cerebral ischemia.
Methods 50 Japanese white rabbits, male or female, were randomly divided into A, B, C three groups, 10 rats in A group, sham operation control group: 20 rats in group B, micro catheter embolization group: 20 rats in group C, as the suture group. Each animal after anesthesia, the exposed side of CCA ECA, ICA, A, was only exposed to artery treatment, B group with 0.50mm diameter of the epidural catheter placement of ICA by CCA, MCA, C group with the same diameter of thread through the same path determination method for the placement of MCA.MCAO, B group was treated by epidural catheter with special drawing production and medical pressure transducer pressure measuring device for monitoring the arterial pressure waveform, the catheter reaches a predetermined depth and arterial pressure waveform disappeared as MCA was successfully closed the criteria of resistance: C group according to the literature according to the length of suture placement as criteria. Two animal groups were in the MCAO one hour after reperfusion injury, and after 24 hours of observation the behavior changes of God According to the situation, PHILIP score were used, (?) from the brain. Head TTC and HE staining effect evaluation model. Results: B, C two models by MCAO 24 hours after the PHILIP score and TTC staining and HE staining electron microscopy showed focal infarction. The success rate of B group was 75%. The C group is 65%. between the two groups had no significant difference (P > 0.05). Conclusion: the micro catheter pressure transducer to accurately determine MCAO monitoring the arterial waveform as the standard of success, the effect is direct and reliable and easy to operate, is a kind of ideal of rabbit cerebral ischemia reperfusion model.

【學(xué)位授予單位】：延邊大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2007
【分類號(hào)】：R-332

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