本文關(guān)鍵詞：產(chǎn)志賀毒素大腸埃希菌多重耐藥分子機(jī)制的研究　出處：《吉林大學(xué)》2006年博士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 產(chǎn)志賀毒素大腸埃希菌 多重耐藥 整合子 基因盒

【摘要】：產(chǎn)志賀毒素大腸埃希菌(Shiga toxin-producing E.coli,STEC)是世界范圍內(nèi)最嚴(yán)重的食源性致病菌。STEC耐藥性產(chǎn)生與傳播已受到世界廣泛關(guān)注。為探討STEC多重耐藥性產(chǎn)生和傳播機(jī)制,本研究采用PCR、接合與轉(zhuǎn)化、克隆測序、Southern blot和酶切圖譜分析等技術(shù),在測定抗菌藥物敏感性的基礎(chǔ)上,對STEC分離株攜帶整合子、R質(zhì)粒和耐藥基因進(jìn)行研究分析。結(jié)果表明多重耐藥STEC染色體、R質(zhì)粒攜帶1500bp ~750bp第1類整合子和dfrA1、aadA1基因盒及sul1、tem、tet、erm等耐藥基因,傳遞對磺胺類、氨基糖苷類、β-內(nèi)酰胺類、四環(huán)素類和紅霉素類等抗菌藥物的耐藥性。發(fā)現(xiàn)了染色體上一種缺陷型及編碼宋內(nèi)志賀菌(S.sonnei)跨膜轉(zhuǎn)運(yùn)蛋白基因的第1類整合子。證明了STEC多重耐藥菌株的出現(xiàn)和流行與農(nóng)業(yè)、畜牧業(yè)將磺胺類、氨基糖苷類等抗菌藥物作為動物生長促進(jìn)劑長期使用有關(guān)。STEC在抗菌藥物的選擇壓力下不斷進(jìn)化,通過整合子與R質(zhì)粒促進(jìn)多重耐藥性的產(chǎn)生和擴(kuò)散。 本研究結(jié)果揭示了STEC在耐藥性表達(dá)及耐藥性傳遞方面的作用機(jī)制,為進(jìn)一步更好地控制STEC基因水平的耐藥性傳播提供重要的科學(xué)依據(jù)。
[Abstract]:Shiga toxin producing Escherichia coli (Shiga toxin-producing, E.coli, STEC) is the world's most serious food borne pathogens, drug resistance and the spread of.STEC has received extensive concern in the world. In order to investigate the multi drug resistance of STEC generation and propagation mechanism, this study adopts PCR, engagement and conversion, cloning and sequencing, restriction map analysis technology Southern blot and enzymes, based on Determination of antimicrobial susceptibility of isolates carrying STEC, integron, research and analysis of R plasmid and resistant genes. The results show that the multidrug resistance of STEC chromosome, R plasmid carrying 1500bp ~ 750bp class first integron and dfrA1 gene cassette, aadA1 and sul1, TEM, Tet, ERM and other resistance genes. Transfer of sulfonamides, aminoglycosides, beta lactams, tetracyclines and erythromycin resistance such as antibiotics. Found a defective chromosome and encoding Shigella sonnei (S.sonnei) membrane Transporter gene class first integron. It is proved that the emergence of multi drug resistance strain STEC and popular with agriculture, animal husbandry will be sulfonamides, as animal growth promoter on.STEC in the long-term use of antimicrobial drug selection pressure evolving antibacterial aminoglycoside, through the production and diffusion of integrons and multiple R plasmid to promote drug resistance.
Our findings reveal the mechanism of STEC in drug resistance and drug resistance delivery, and provide important scientific evidence for further better control of drug resistance in STEC gene level.

【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級別】：博士
【學(xué)位授予年份】：2006
【分類號】：R378.2

【引證文獻(xiàn)】

相關(guān)博士學(xué)位論文 前1條

1 張若文;燒傷病房耐碳青霉烯類銅綠假單胞菌耐藥機(jī)制及分子流行病學(xué)研究[D];吉林大學(xué);2012年

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本文編號：1370064