本文關鍵詞：產志賀毒素大腸埃希菌多重耐藥分子機制的研究　出處：《吉林大學》2006年博士論文　論文類型：學位論文

  更多相關文章： 產志賀毒素大腸埃希菌 多重耐藥 整合子 基因盒

【摘要】：產志賀毒素大腸埃希菌(Shiga toxin-producing E.coli,STEC)是世界范圍內最嚴重的食源性致病菌。STEC耐藥性產生與傳播已受到世界廣泛關注。為探討STEC多重耐藥性產生和傳播機制,本研究采用PCR、接合與轉化、克隆測序、Southern blot和酶切圖譜分析等技術,在測定抗菌藥物敏感性的基礎上,對STEC分離株攜帶整合子、R質粒和耐藥基因進行研究分析。結果表明多重耐藥STEC染色體、R質粒攜帶1500bp ~750bp第1類整合子和dfrA1、aadA1基因盒及sul1、tem、tet、erm等耐藥基因,傳遞對磺胺類、氨基糖苷類、β-內酰胺類、四環(huán)素類和紅霉素類等抗菌藥物的耐藥性。發(fā)現了染色體上一種缺陷型及編碼宋內志賀菌(S.sonnei)跨膜轉運蛋白基因的第1類整合子。證明了STEC多重耐藥菌株的出現和流行與農業(yè)、畜牧業(yè)將磺胺類、氨基糖苷類等抗菌藥物作為動物生長促進劑長期使用有關。STEC在抗菌藥物的選擇壓力下不斷進化,通過整合子與R質粒促進多重耐藥性的產生和擴散。 本研究結果揭示了STEC在耐藥性表達及耐藥性傳遞方面的作用機制,為進一步更好地控制STEC基因水平的耐藥性傳播提供重要的科學依據。
[Abstract]:Shiga toxin producing Escherichia coli (Shiga toxin-producing, E.coli, STEC) is the world's most serious food borne pathogens, drug resistance and the spread of.STEC has received extensive concern in the world. In order to investigate the multi drug resistance of STEC generation and propagation mechanism, this study adopts PCR, engagement and conversion, cloning and sequencing, restriction map analysis technology Southern blot and enzymes, based on Determination of antimicrobial susceptibility of isolates carrying STEC, integron, research and analysis of R plasmid and resistant genes. The results show that the multidrug resistance of STEC chromosome, R plasmid carrying 1500bp ~ 750bp class first integron and dfrA1 gene cassette, aadA1 and sul1, TEM, Tet, ERM and other resistance genes. Transfer of sulfonamides, aminoglycosides, beta lactams, tetracyclines and erythromycin resistance such as antibiotics. Found a defective chromosome and encoding Shigella sonnei (S.sonnei) membrane Transporter gene class first integron. It is proved that the emergence of multi drug resistance strain STEC and popular with agriculture, animal husbandry will be sulfonamides, as animal growth promoter on.STEC in the long-term use of antimicrobial drug selection pressure evolving antibacterial aminoglycoside, through the production and diffusion of integrons and multiple R plasmid to promote drug resistance.
Our findings reveal the mechanism of STEC in drug resistance and drug resistance delivery, and provide important scientific evidence for further better control of drug resistance in STEC gene level.

【學位授予單位】：吉林大學
【學位級別】：博士
【學位授予年份】：2006
【分類號】：R378.2

【引證文獻】

相關博士學位論文 前1條

1 張若文;燒傷病房耐碳青霉烯類銅綠假單胞菌耐藥機制及分子流行病學研究[D];吉林大學;2012年

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本文編號：1370064