本文關(guān)鍵詞：水通道蛋白在人子宮內(nèi)膜中的表達(dá)及其調(diào)節(jié)機(jī)制的研究　出處：《浙江大學(xué)》2006年博士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 子宮內(nèi)膜 女(雌)性 水通道蛋白 性激素類 子宮內(nèi)膜 水通道蛋白 女(雌)性 細(xì)胞培養(yǎng) 子宮內(nèi)膜 水通道蛋白 阻斷

【摘要】：自從1992年Agre Peter教授第一次從人紅細(xì)胞膜中分離和鑒定出水通道蛋白1(aquaporin 1,AQP1)以來(lái),后續(xù)研究證明水通道蛋白是一個(gè)大家族,其成員廣泛分布于動(dòng)物、植物和微生物體內(nèi),介導(dǎo)水分子的快速跨膜轉(zhuǎn)運(yùn),促進(jìn)細(xì)胞膜水滲透性增加;同時(shí)發(fā)揮調(diào)節(jié)細(xì)胞容積和細(xì)胞內(nèi)滲透壓以及內(nèi)環(huán)境穩(wěn)態(tài)的重要生理作用,這一發(fā)現(xiàn)被譽(yù)為生命科學(xué)研究的一個(gè)里程碑,Agre Peter教授因此榮獲2003年諾貝爾化學(xué)獎(jiǎng);生殖生理和生殖病理的研究一直是人們關(guān)注的生殖領(lǐng)域熱點(diǎn)問(wèn)題;生殖過(guò)程的許多方面涉及到液體的改變,如卵泡的生長(zhǎng)發(fā)育、胚泡的發(fā)育、精子的發(fā)生成熟、精液的形成以及子宮內(nèi)膜和子宮腔液的周期性變化,均與液體的分泌和吸收有著密切的關(guān)系;研究證實(shí)有多種水通道蛋白豐富地表達(dá)于睪丸、精子、卵泡、子宮、胎盤(pán)、胎膜等組織,因此,研究水通道蛋白在生殖系統(tǒng)的表達(dá)、作用及其調(diào)節(jié)機(jī)制具有重要的生理學(xué)意義;然而,水通道蛋白是否存在于人的子宮內(nèi)膜中?若存在,其表達(dá)特性如何?是否與月經(jīng)周期存在一定的關(guān)系?是否受到卵巢甾體激素的調(diào)節(jié)?這一系列問(wèn)題至今未見(jiàn)報(bào)道。本研究旨在探討水通道蛋白在人月經(jīng)周期子宮內(nèi)膜中的分布特征和表達(dá)規(guī)律,并通過(guò)體外刺激實(shí)驗(yàn)研究雌孕激素對(duì)人子宮內(nèi)膜上皮細(xì)胞水通道蛋白2(aquaporin 2,AQP2)表達(dá)的調(diào)節(jié),以探討AQP2在人不同月經(jīng)周期子宮內(nèi)膜上表達(dá)的變化規(guī)律及其調(diào)節(jié)機(jī)制。
[Abstract]:Since 1992, Agre professor Peter first isolated from human erythrocyte membrane and identification of water channel protein 1 (aquaporin 1, AQP1), subsequent studies show that water channel protein is a big family, whose members are widely distributed in animal, plants and microorganisms, rapid transmembrane transport mediated by water molecules, promote cell membrane water permeability increased; at the same time play an important physiological role of osmotic pressure and homeostasis regulation of cell volume and intracellular, this discovery is a milepost known as life science research, Professor Peter Agre won the 2003 Nobel prize in chemistry; research on reproductive physiology and reproductive pathology has been the focus that people pay close attention to reproductive field many aspects of the reproductive process; related to fluid change, such as follicular development, blastocyst development, spermatogenesis, sperm formation and endometrial and uterine cavity The periodic change of the liquid, and the liquid absorption and secretion are closely related; research shows that many water channel protein abundantly expressed in testis, sperm, follicle, uterus, placenta, fetal membranes and other organizations, therefore, the research of water channel protein expression in the reproductive system, as well as adjustment mechanism has important physiological significance; however, the water channel protein exists in human endometrium? If there is, its expression characteristics? Whether there is a certain relationship with the menstrual cycle? Is regulated by ovarian steroid hormone? This series of questions has not been reported. This study aimed to investigate the distribution of water channel protein in human endometrium during menstrual cycle and the expression of the law, and through the experimental study of in vitro stimulation of estrogen and progesterone on endometrial epithelial cells of aquaporin 2 (aquaporin 2, AQP2) regulating the expression of AQP2 in different people, to explore Changes in the expression of endometrium on the menstrual cycle and its regulatory mechanism.

【學(xué)位授予單位】：浙江大學(xué)
【學(xué)位級(jí)別】：博士
【學(xué)位授予年份】：2006
【分類號(hào)】：R33

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本文編號(hào)：1369604