本文關(guān)鍵詞：外周和脊髓NMDA受體在Formalin引起的脊髓小膠質(zhì)細(xì)胞活化和長時程痛敏中的作用　出處：《山西醫(yī)科大學(xué)》2005年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： Formalin 小膠質(zhì)細(xì)胞 NMDA 受體 MK-801 長時程痛敏

【摘要】：經(jīng)典的formalin 痛模型即腳掌注射formalin 引起的持續(xù)約1 小時的雙相性痛行為反應(yīng)。由于能較好地模擬臨床急性炎癥痛及能采用定性和半定量的指標(biāo)對痛反應(yīng)進(jìn)行測量,因而被廣泛應(yīng)用于疼痛機(jī)制的研究。新近的研究顯示,formalin 皮下注射除引起上述急性炎性痛外,還可以引起持續(xù)性的痛敏狀態(tài),如持續(xù)約4 周的熱輻射痛敏反應(yīng)及同時觀察到的脊髓背角小膠質(zhì)細(xì)胞的激活。在以往的實驗中,本研究室及其他研究者的觀察都證實,NMDA 受體在formalin 引起的急性炎性痛中發(fā)揮重要作用。本實驗在外周注射formalin 的基礎(chǔ)上,通過痛行為觀察(熱輻射刺激縮足反射潛伏期)、對小膠質(zhì)細(xì)胞活化增殖的免疫組化檢測(OX-42 陽性表達(dá)),結(jié)合外周及鞘內(nèi)注射NMDA 受體拮抗劑MK-801,試圖證實外周與脊髓NMDA 受體在formalin 所致的長時程痛敏及脊髓小膠質(zhì)細(xì)胞的活化中發(fā)揮重要作用。 實驗在Sprague-Dawley 雄性大鼠(90~100g)進(jìn)行。本實驗分兩部分,第一部分是通過外周注入MK-801 觀察外周傳入感覺神經(jīng)末稍上NMDA 受體在formalin 引起的長時程痛敏反應(yīng)及脊髓小膠質(zhì)細(xì)胞活化中的作用。包括免疫組化實驗和行為學(xué)觀察。在免疫組化實驗中,根據(jù)給藥方法的不同,分為多次給藥組和單次給藥組(n=6)。多次給藥組中,在動物兩側(cè)腳掌注入formalin 前15 分鐘,將NMDA 受體的拮抗劑MK-801(分為三個劑量組即10 nmol、30 nmol、50 nmol)注入左側(cè)腳掌皮下,而在右側(cè)注入生理鹽水(NS),并且在formalin 注射后的第1 天、第2 天、第3 天、第4 天分別重復(fù)給予原劑量的MK-801或NS。在注射formalin后第7天進(jìn)行OX-42表達(dá)的免疫組化檢測,對比背角兩側(cè)小膠質(zhì)細(xì)胞的免疫組化反應(yīng),進(jìn)行圖像分析及統(tǒng)計學(xué)處理。單次給藥組也分為三個劑量組即50 nmol、100 nmol、150 nmol,與多次給藥組不同的是注射formalin 前15 分鐘一次全量給予MK-801 或NS,到第7 天進(jìn)行免疫組化檢測。在痛行為實驗觀察中,分為實驗組(30 nmol MK-801+formalin)和對照組(NS+formalin),采用多
[Abstract]:Diphasic pain behavior response formalin pain model is the classic peripheral injection of formalin induced lasted about 1 hours. Because it can mimic the clinical acute inflammatory pain and can use qualitative and semi quantitative indicators of pain response were measured, so it is widely used in the research of the mechanism of pain. Recent studies have shown that formalin in addition to the subcutaneous injection caused by acute inflammatory pain, can also cause pain sensitive state persistence, activate the heat radiation of pain sensitivity as lasts for 4 weeks and also observed in spinal microglia. In previous experiments, observe the research lab and other researchers have confirmed and play an important role in formalin induced NMDA receptor in acute inflammatory pain. In this experiment the peripheral injection of formalin on pain behavior through the observation (thermal withdrawal latency), the activation of microglia increased Immunohistochemistry (OX-42 positive expression), combined with peripheral and intrathecal injection of NMDA receptor antagonist MK-801, attempts to confirm the role of NMDA receptors in peripheral and spinal cord in the long term pain sensitivity induced by formalin and the activation of spinal microglia.
Experiment in male Sprague-Dawley rats (90~100g). The experiment is divided into two parts. The first part is through the peripheral injection of MK-801 was observed in the peripheral role of Cheng Tongmin reaction and spinal microglia activation of afferent sensory nerve endings of NMDA receptors in formalin induced long. Including immunohistochemical experiments and behavioral observation in the immunohistochemical experiments, according to the different methods, divided into multiple dosing group and single drug group (n=6). Multiple dosing group, formalin injection in animal feet on both sides of the first 15 minutes, the NMDA receptor antagonist MK-801 (divided into three groups: 10 nmol 30, nmol, 50 nmol) subcutaneously injected into the left foot, while the injection of normal saline (NS) on the right side, and on the first day after formalin injection for second days, third days, fourth days respectively to the original repeated doses of MK-801 or NS. in seventh days after the injection of formalin OX-42 The group of immune detection, immune group comparison of dorsal horn microglia on both sides of the reaction, image analysis and statistical treatment. The single dose group was also divided into three groups: 50 nmol, 100 nmol, 150 nmol, and multiple dose group is 15 minutes before the first injection of formalin the full amount of MK-801 or NS, to seventh days by immunohistochemical detection. In pain behavior experiment, divided into experimental group (30 nmol MK-801+formalin) and control group (NS+formalin), the

【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2005
【分類號】：R338

【引證文獻(xiàn)】

相關(guān)碩士學(xué)位論文 前1條

1 周雙瓊;小劑量氯胺酮和嗎啡對慢性神經(jīng)痛大鼠P2X_4受體表達(dá)的影響[D];中南大學(xué);2007年

，

本文編號：1366669