本文關(guān)鍵詞：流感病毒核酸疫苗母源性抗體的保護性與干擾性研究　出處：《湖南師范大學(xué)》2005年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 流感病毒 血凝素核酸疫苗 神經(jīng)氨酸酶核酸疫苗 滅活疫苗 母源性抗體

【摘要】：本論文共有兩部分組成:(1) 流感病毒血凝素(hemagglutinin,HA)、神經(jīng)氨酸酶(neuraminidase,NA)核酸疫苗誘導(dǎo)的母源性抗體保護子鼠抗致死量流感病毒攻擊能力的研究;(2) 在子鼠體內(nèi)有母源性抗體的情況下,給子鼠接種不同的流感疫苗,尋找一種既能解除母源性抗體干擾作用,又能很好的保護子鼠抵御致死量流感病毒攻擊的新型免疫途徑。 嬰幼兒免疫系統(tǒng)不成熟,對接種疫苗存在耐受性、危險性和免疫應(yīng)答能力低下等問題,因此母源性抗體成為保護嬰幼兒早期抵御流感病毒感染的重要方式之一。實驗中我們分別用HA、NA、(HA+NA)的混和疫苗免疫6～8周齡的BALB/c雌鼠兩次,二免后七天,將雌鼠與未經(jīng)免疫的BALB/c雄鼠交配,所產(chǎn)子鼠在不同的周齡分別用同型致死量流感病毒A/P R/8/34進行攻擊,通過測定子鼠存活率、體內(nèi)抗體滴度及肺部病毒殘余滴度,得出結(jié)論:HA DNA接種母體能夠保護2周齡幼齡鼠抵御致死量流感病毒的攻擊,NA DNA接種母體能夠保護2,3,4周齡子鼠抵御致死量流感病毒的攻擊,(HA+NA)混合疫苗接種母體不僅能夠保護幼齡鼠而且能夠保護5,6周齡的成年子鼠抵御致死量流感病毒的攻擊。這說明流感病毒HA DNA、NA DNA能用于母體免疫保護嬰幼兒抵御致死量流感病毒攻擊,兩種疫苗聯(lián)合應(yīng)用對子代的保護效果更佳。 母源性抗體一方面能夠保護新生兒和嬰幼兒早期抵御病毒和細菌的感染,但另一方面它能在嬰幼兒體內(nèi)持續(xù)存在一段時間,抑制嬰幼兒疫苗接種后主動免疫應(yīng)答的建立。因此,尋
[Abstract]:This thesis consists of two parts: (1) influenza virus hemagglutinin neuraminidase (hemagglutinin, HA), (neuraminidase, NA) of DNA vaccine induced by maternal antibodies to protect rats against lethal dose virus attack ability; (2) in the rat in vivo maternal antibody, were inoculated to different influenza vaccine, to find a way to relieve maternal antibody interference, and new way of immune good protection against mouse lethal dose of influenza virus attacks.
The infant immune system is not mature, there is tolerance to vaccination, the problem of danger and the immune response ability is low, so the maternal antibody has become one of the important ways to protect infants against influenza virus infection. In the experiment we used HA NA (HA+NA), respectively, the mixed vaccine 6 to 8 weeks of age BALB/c two female rats, two free after seven days, the female rats with non immunized BALB/c mice mating offspring in different weeks respectively with the same type of a lethal dose of influenza virus A/P R/8/34 attack, through the determination of offspring survival rate, the antibody titer in the lungs and the residual virus titer, concluded: HA DNA inoculation can protect young maternal rats aged 2 weeks against a lethal dose of influenza virus attacks, NA DNA can protect the 2,3,4 maternal vaccination week old offspring rats against lethal dose of influenza viruses (HA+NA), mixed vaccine can not only protect the mother's body The young mice and can protect the 5,6 week old adult offspring against a lethal dose of influenza virus attack. This shows that the influenza virus HA DNA, NA DNA can be used for the maternal immune protection of infants against a lethal dose of influenza virus attacks, two kinds of better protection effect of combined application of vaccine in offspring.
On the one hand, maternal antibodies can protect newborns and infants from viral and bacterial infections, but on the other hand, they can persist in infants for a period of time, and inhibit the establishment of active immune response after vaccination.

【學(xué)位授予單位】：湖南師范大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2005
【分類號】：R392

【引證文獻】

相關(guān)碩士學(xué)位論文 前1條

1 張步彩;雞柔嫩艾美耳球蟲（E.tenella）DNA疫苗pEtK2-IL-2免疫程序、交叉免疫及穩(wěn)定性研究[D];南京農(nóng)業(yè)大學(xué);2006年

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本文編號：1360535