本文關(guān)鍵詞：桉葉油對維甲酸干擾大鼠胚胎植入及生長發(fā)育的影響　出處：《解剖學(xué)報(bào)》2014年01期 　論文類型：期刊論文

  更多相關(guān)文章： 桉葉油 維甲酸 致畸 胚胎發(fā)育 生殖毒性實(shí)驗(yàn) 大鼠

【摘要】：目的探討桉葉油對維甲酸干擾大鼠胚胎植入及生長發(fā)育的影響。方法 SD孕鼠42只,隨機(jī)分為6組,FA組正常對照組、溶劑組(花生油+RA)、3個(gè)實(shí)驗(yàn)組(桉葉油高、中、低劑量+RA)及RA組。溶劑組用花生油2ml/只于孕第7~14天灌胃,每天1次,孕第10天維甲酸40mg/kg灌胃1次。桉葉油3個(gè)劑量組分別用300mg/kg、200mg/kg、100mg/kg的桉葉油于孕第7~14天灌胃,每天1次,孕第10天維甲酸40mg/kg灌胃1次。RA組用40mg/kg的維甲酸于孕第10天灌胃1次。正常對照組給予自由攝食飲水。各組于孕21d早上處死孕鼠,取胚胎,記錄孕鼠體重,子宮重,胎盤重。計(jì)胚胎植入總數(shù),吸收胎數(shù)、活胎數(shù)、死胎數(shù)。觀察胚胎外形,并測量胎鼠體重、身長、尾長。結(jié)果正常對照組、桉葉油+RA和溶劑+RA組孕鼠增重、子宮重、胎盤重與RA組比較均無差異;溶劑+RA組的孕鼠的胎盤重較其他各組低,與RA和桉葉油+RA各組比較差異無顯著性,與正常對照組比較有差異(P0.05)。正常對照組的活胎率[(97.3±4.6)%]較灌胃維甲酸各組的活胎率高(P0.05),吸收胎率[(2.6±4.6)%]和畸形率(0)較灌胃維甲酸各組低(P0.05),死胎率為0;在灌胃維甲酸各組中,桉葉油高[(79.6±14.5)%]、中[(67.6±30.8)%]劑量組的活胎率較RA[(58.6±26.6)%]組高,但比較無差異;畸胎率[(44.5±41.6)%;(57.5±35.1)%]較RA[(68.1±43.6)%]組低,但差異無統(tǒng)計(jì)學(xué)意義。灌胃維甲酸各組的吸收胎率比較無差異。正常對照組的胎鼠體重、身長、尾長均值明顯高于灌胃維甲酸各組胎鼠的體重、身長、尾長均值(P0.05);在灌胃維甲酸各組中,桉葉油各組胎鼠體重、身長的均值明顯高于RA組和溶劑對照組(P0.05);溶劑對照組胎鼠體重、身長、尾長與RA組比較無差異;桉葉油各組胎鼠尾長的均值均高于RA組,但比較無差異。結(jié)論維甲酸干擾了大鼠胚胎的植入和發(fā)育,桉葉油對RA干擾大鼠胚胎植入有一定的拮抗作用,對RA引起的胎鼠生長發(fā)育遲緩有一定的抑制作用。
[Abstract]:Objective to investigate the effects of eucalyptus oil on the embryo implantation and growth of rats disturbed by retinoic acid. Methods 42 SD pregnant rats were randomly divided into 6 groups, group FA normal control group, solvent group (peanut oil +RA), 3 experimental groups (Eucalyptus oil high, medium and low dose +RA) and RA group. Solvent group with peanut oil 2ml/ on pregnancy day 7~14 orally, 1 times a day, at the tenth day of formic acid 40mg/kg by gavage for 1 times. The eucalyptus oil 3 dose groups respectively with eucalyptus oil 300mg/kg, 200mg/kg, 100mg/kg by intragastric administration in pregnancy 7~14 days, 1 times a day, at the tenth day of formic acid 40mg/kg by gavage for 1 times. In group RA, the retinoic acid of 40mg/kg was administered for 1 times on the tenth day of pregnancy. The normal control group was given free feeding and drinking water. The pregnant rats were killed in the morning 21d, and the embryos were taken to record the weight of the pregnant mice, the weight of the uterus and the weight of the placenta. The total number of embryo implantation, the number of absorbed tyres, the number of living fetus and the number of stillbirth were counted. The shape of the embryo was observed and the body weight, length and tail length of the fetal rats were measured. The results of the normal control group, +RA of eucalyptus oil and solvent group +RA pregnant rats weight, uterine weight, placental weight and RA group showed no difference; placenta solvent +RA group of pregnant rats than in the other groups of low weight, compared with RA and Eucalyptus +RA groups had no significant difference, compared with normal control group there are differences (P0.05). The rate of live births in normal control group [(97.3 + 4.6) when compared with the live fetuses intragastric retinoic acid group (P0.05), high rate of absorption rate [(2.6 + 4.6) and (0) when compared with the deformity rate of intragastric administration of retinoic acid (P0.05) were low, the stillbirth rate was 0; in the stomach retinoic acid group, eucalyptus oil high [(79.6 + 14.5) when, in [(67.6 + 30.8) live births when dose rate is RA[(58.6 + 26.6) when it was higher, but there is no difference; teratogenic rate of [(44.5 + 41.6)%; (57.5 + 35.1) when compared with the RA[(68.1 + 43.6) when it was lower, but the difference was not statistically significant. There was no difference in the fetal rate of tretinoin by gavage. The normal control group of fetal rat body weight, body length and tail length were obviously higher than average intragastric retinoic acid fetal body weight, body length and tail length of mean (P0.05); in intragastric administration of retinoic acid in each group, the mean weight and length of eucalyptus oil in all groups was significantly higher than that of RA group and solvent control group (P0.05) the solvent control group; the fetal body weight, body length and tail length of RA group had no significant difference between the mean leaf oil; fetal rat tail length of Eucalyptus were higher than RA group, but no difference. Conclusion retinoic acid interferes with the implantation and development of rat embryos. Eucalyptus leaf oil has some antagonistic effects on RA interference in rat embryo implantation, and inhibits the growth retardation of fetal rats induced by RA.
【作者單位】： 遵義醫(yī)學(xué)院珠海校區(qū)解剖學(xué)與組織學(xué)胚胎學(xué)教研室;
【基金】：貴州省社會(huì)攻關(guān)資助項(xiàng)目[黔科合SY字(2011)3054號(hào)]
【分類號(hào)】：R321
【正文快照】： 桉樹葉是民間常用的草藥,桉葉油[1]是從桉樹提取出的芳香揮發(fā)性油,組分繁多,其中桉葉素含量可達(dá)90%以上。主要化學(xué)成分是萜類、萜醇、醚、酮類和酯等含氧化合物。具有殺蟲、抗菌、抗炎、促滲、抗氧化及防腐等藥理作用。最新研究資料顯示,桉葉油具有舒張氣管和血管平滑肌、心肌

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