The actin nucleator actin-related protein complex（Arp2/3） is composed of seven subunits: Arp2,Arp3, p40/ARPC1（P40）, p34/ARPC2（P34）, p21/ARPC3（P21）, p20/ARPC4（P20）, and p16/ARPC5（P16）. Arp2/3 plays crucial roles in a variety of cellular activities through regulation of actin polymerization. Autographa californica multiple nucleopolyhedrovirus（Ac MNPV）, one of the beststudied alphabaculoviruses, induces Arp2/3 nuclear relocation and mediates nuclear actin polymerization to assist in virus replicat... 

【文章來源】：Virologica Sinica. 2016,31(06)CSCD

【文章頁數(shù)】：10 頁

【文章目錄】：
INTRODUCTION
MATERIALS AND METHODS
    Cell culture and transfection
    Cloning of Arp2/3 subunits and plasmid construction
    Immunoprecipitation
    Immunofluorescence staining and densitometry assay
RESULTS
    Sequence of Arp2/3 subunits of S.frugiperda
    Ac34 showed different capabilities with respect to nuclear relocation of different Arp2/3 subunits
    Ac34 had no influence on the subcellular distribution of H.sapiens Arp2/3
    Interaction of the Ac34 and Arp2/3 subunits
DISCUSSION
COMPLIANCE WITH ETHICS GUIDELINES
AUTHOR CONTRIBUTIONS



本文編號：3136426