本文關鍵詞：美洲黑楊對冬季水淹的性別特異性響應　出處：《中國科學院研究生院(武漢植物園)》2015年碩士論文　論文類型：學位論文

  更多相關文章： 水淹 雌雄 楊樹 生理生化 蛋白質組學

【摘要】：三峽水庫受“冬季蓄水,夏季泄洪”人工水位調節(jié)的影響,形成了垂直落差達30 m的水庫消落帶,造成植物多樣性急劇下降,從而引起一系列的生態(tài)問題。消落帶防護林對維護庫岸穩(wěn)定、減少水土流失、保持生態(tài)景觀以及水體凈化等方面起著重要作用,因此在三峽水庫上部消落帶構建生態(tài)防護林具有重要的意義。三峽水庫消落帶生態(tài)防護林建設的適宜樹種必須具備耐冬季水淹、適應夏季干旱、改良土壤的能力。楊樹具有適應性強,生長速度快和豐產(chǎn)等特性,具有重要的生態(tài)及經(jīng)濟價值,常作為極端環(huán)境生態(tài)修復的先鋒樹種。前人研究表明,美洲黑楊(Populus deltoides)具備較強的耐水淹能力。另外,楊樹為雌雄異株植物,前人研究表明其雌雄植株對非生物脅迫存在性別差異響應。本研究以美洲黑楊為研究材料,模擬三峽水庫消落帶冬季水淹環(huán)境條件,研究雌雄植株對冬季水淹的性別差異性響應。相關研究成果為三峽庫區(qū)消落帶生態(tài)防護林建設提供理論依據(jù),主要結果如下：1通用提取緩沖液對脅迫相關生理生化指標提取效率的研究為節(jié)約試驗材料和人工成本,研制一種適用于多種生理指標的通用提取液十分有必要。由于大部分生理指標提取液為磷酸緩沖液或Tris-HCl緩沖液且差異不大,因此比較通用磷酸緩沖液、通用Tris-HCl緩沖液及專一性提取液對9種生理指標的提取效率,研究發(fā)現(xiàn)100 mmol/L的通用磷酸緩沖液適用于GR, GPx, CAT, SOD, O2-,可溶性蛋白和游離脯氨酸等大部分生理指標的提取測定。2冬季水淹下美洲黑楊雌雄植株生理生化的響應差異經(jīng)過130天冬季水淹脅迫后,所有的雌雄植株能完全存活,表明美洲黑楊具備較強的耐水淹能力。但是,雌雄植株在生長發(fā)育、生理生化方面的響應存在性別差異。正常水分狀況下,雌株在還原性糖,O2-, .OH, APx, CAT, A及WUEi本底含量優(yōu)于雄株；而在水淹狀況下,雌株在Ci, H2O2, MDA,株高和基徑等方面受到的負面影響大于雄株；相反,雄株在GSH, SOD和POD對水淹脅迫正反饋優(yōu)于雌株；另外,雌雄植株在葉綠素熒光方面對水淹脅迫前后的差異不顯著。3冬季水淹下美洲黑楊雌雄植株的蛋白質組學分析利用同位素標記相對和絕對定量(isobaric tags for relative and absolute quantitation, iTRAQ)技術,對冬季水淹條件下美洲黑楊雌雄植株的葉片全蛋白質組學進行了分析,通過兩兩比較,共鑒定了757個至少在一個組合中有顯著性差異的蛋白。其中25個是雌株特異性蛋白,在水淹條件下,雄株中表達量比雌株高,而且在雌株中受到水淹誘導后上調。另有80個蛋白在水淹以后,雄株中表達量比雌株低,而且在雌株中受水淹誘導下調。這105個蛋白有可能直接參與了雌雄植株對水淹脅迫的應答。相關鑒定的蛋白主要參與了Transcription, Replication, Recombination and Repair, Post Modification, Protein Turnover, Chaperones, Coenzyme Transport及Metabolism等途徑。以上結果表明,100 mmol/L的通用磷酸緩沖液適用于同時提取GR, GPx, CAT, SOD, O2-,可溶性蛋白和游離脯氨酸等大部分生理指標。美洲黑楊在130天水淹處理以后,所有的雌雄植株能完全存活,表明美洲黑楊具備較強的耐水淹能力。但是,雌雄植株在生長發(fā)育、生理生化及蛋白等方面的響應存在性別差異,雄株比雌株有著更強的耐水淹能力,該研究成果能為三峽水庫消落帶楊樹生態(tài)防護林建設提供科學指導。
[Abstract]:The Three Gorges Reservoir by water in winter, the summer flood "artificial water level regulation, forming a vertical drop of 30 m reservoir riparian zone, resulting in plant diversity decreased rapidly, which caused a series of ecological problems. With Shelterbelts on the maintenance of stability of reservoir drawdown, reduce soil erosion, maintain plays an important role the ecological landscape and water purification and so on, so the upper riparian zone in the Three Gorges reservoir ecological protection forest construction has important significance. A suitable tree species of the Three Gorges reservoir, the construction of ecological protection forest must have resistance to winter flooding, summer drought, soil improvement ability. Poplar has strong adaptability, fast growth and high yield characteristics that has important ecological and economic value, often as a pioneer species in the extreme environment of ecological restoration. Previous studies showed that America Black Poplar (Populus deltoides) have stronger resistance to flooding. On the other Outside, poplar is dioecious, previous studies showed that there are gender differences in the responses of male and female plants to abiotic stress. In this study, black poplar of America as research materials, simulation of the Three Gorges reservoirtotal winter flooding conditions of winter flooding on gender differences in male and female plants response. Related research results with the construction of ecological protection forest to provide a theoretical basis for the Three Gorges Reservoir area, the main results are as follows: 1 Research on the general extraction buffer on the extraction efficiency of stress related physiological and biochemical indexes in order to save the material and labor costs, it is necessary to develop a suitable for various physiological indicators of general extract. Due to extract most of the physiological indexes of phosphate buffer or Tris-HCl buffer the liquid and the difference is not so common, phosphate buffer, Tris-HCl buffer and the specificity of the extracted effect on 9 kinds of physiological index rate The study found that general, 100 mmol/L phosphate buffer for GR, GPx, CAT, SOD, O2-, extraction of most physiological indexes of soluble protein and free proline determination response of Populus deltoides male and female plants physiological and biochemical.2 winter flooding difference after 130 days of winter flooding stress, male and female plants can all survived completely. That America black poplar have strong waterlogging tolerance. However, male and female plants in growth and development, there are gender differences in response to physiological and biochemical aspects. The normal water condition, the female plant in the reducing sugar, O2-,.OH, APx, CAT, A and WUEi is better than that of the background content of male plants; while in the flooded condition. The female plant in Ci, H2O2, MDA, negative effects on plant height and basal diameter as much more than the male plants; on the contrary, the male plant in GSH, SOD and POD on the flooding stress positive feedback is better than the female line; in addition, male and female plants in the chlorophyll fluorescence of water stress No significant difference before and after the forced.3 winter flooding under Populus deltoides male and female plants proteomic analysis using isobaric tags for relative and absolute quantitation (isobaric tags for relative and absolute quantitation iTRAQ) technology, the winter flooding conditions of Populus deltoides male plant leaf proteome was analyzed by comparison of 22, 757 proteins were identified at least significant difference in a group. 25 of them are female specific protein, in flooded conditions, the expression in the male plants than female plants, and induced by flooding after increase in female plants. Another 80 protein in flooded after expression in the male plants than female plants, and in female plants by flooding induced down-regulation. These 105 proteins may be directly involved in the male and female plants to flooding stress response. The identified proteins mainly involved in Transcription, Repl Ication Recombination, and Repair, Post Modification, Protein Turnover, Chaperones Coenzyme, Transport and Metabolism. The results above indicated that the general 100 mmol/L phosphate buffer was suitable for the simultaneous extraction of GR, GPx, CAT, SOD, O2-, most physiological indexes of soluble protein and free proline. Deltoides in 130 days flooded after all, male and female plants can survive, that America black poplar have strong waterlogging tolerance. However, male and female plants in growth and development, physiological and biochemical aspects of protein response due to gender differences, male plants than female plants have a stronger flooding-tolerant ability, the research results can provide scientific guidance for the Three Gorges reservoirtotal poplar ecological shelterbelt construction.

【學位授予單位】：中國科學院研究生院(武漢植物園)
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：S792.11

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相關期刊論文 前1條

1 薛艷紅;陳芳清;樊大勇;謝宗強;;宜昌黃楊對夏季淹水的生理生態(tài)學響應[J];生物多樣性;2007年05期

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本文編號：1412620