本文關(guān)鍵詞：曠場實(shí)驗(yàn)，由筆耕文化傳播整理發(fā)布。

摘要：

目的 探討曠場實(shí)驗(yàn)作為昆明小鼠行為學(xué)評(píng)價(jià)方法的重測信度.方法 成年雄性KM小鼠放入曠場箱正中央格,攝像系統(tǒng)記錄5 min的行為變化,實(shí)驗(yàn)間隔1周進(jìn)行2次;通過組內(nèi)相關(guān)系數(shù)(ICC)和一致性系數(shù)(Kappa)對(duì)曠場實(shí)驗(yàn)進(jìn)行重測信度檢驗(yàn),參數(shù)如下:中央格數(shù)、外周格數(shù)、中央格百分比、水平運(yùn)動(dòng)、垂直運(yùn)動(dòng)、總得分及糞便粒數(shù).結(jié)果 ①ICC結(jié)果表明,除糞便粒數(shù)(ICC=0.376,P＜0.05)外,中央格數(shù)(ICC=0.578,P＜0.01)、外周格數(shù)(ICC=0.615,P＜0.01)、中央格百分比(ICC=0.523,P＜0.01)、水平運(yùn)動(dòng)(ICC=0.607,P＜0.01)、垂直運(yùn)動(dòng)(ICC=0.606,P＜0.01)及總得分(ICC=0.902,P＜0.01)均顯示具有良好重測信度,尤其是總得分初測與重測具有良好相關(guān)性(r=0.815,P＜0.01).②Kappa結(jié)果表明,僅總得分(Kappa=0.680,P＜0.01)顯示OFT具有良好重測信度.③t檢驗(yàn)結(jié)果表明,外周格數(shù)(t=3.01,P＜0.01)、水平運(yùn)動(dòng)(t=3.34,P＜0.01)和總得分(t=7.86,P＜0.001)初測與重測結(jié)果差異具有統(tǒng)計(jì)學(xué)意義.結(jié)論 總得分可以作為昆明小鼠OFT的一個(gè)穩(wěn)定參數(shù),具有較好的重測信度.

Abstract： 

Objective To explore the test-retest reliability of the open field test,as a method for ethology in Kunming mice. Methods The behavior of adult male Kunming mice in the open field test was recorded twice,once for five minutes, with a one-week inter-trial interval. The following parameters were evaluated:number of central squares; number of peripheral squares; percent of central squares; number of horizontal squares; number of rearings; total score; and number of fecal boli. Subsequently,the intraclass correlation coefficient (ICC) and the agreement index (Kappa) were calculated for each of these parameters. Results Both ICC and Kappa for total score( ICC =0.902, P＜0.01; Kappa =0.680, P＜0.01; r=0.815, P＜0.01 ) showed a good reliability and correlation between the test trials. Only ICC for number of central squares ( ICC = 0. 578, P ＜ 0.01 ), number of peripheral squares( ICC =0.615, P＜0.01 ) ,percent of central squares(ICC=0.523, P＜0.01) ,number of horizontal squares( ICC = 0. 607, P ＜ 0.01 ) and number of rearings ( ICC = 0. 606, P ＜ 0.01 ) indicated a fair reliability between the test trials. Neither ICC nor Kappa for number of fecal boli displayed a significant reliability between the test trials. The difference of these parameters between test and retest, such as number of peripheral squares( t =3.01, P＜0.01 ),number of horizontal squares( t = 3.34, P ＜ 0.01 ) and total score( t = 7.86, P ＜ 0.01 ) were significant. Conclusion The parameter,total score for Kunming mice in the open field test,is found to be stable over time, and indicates a good reliability with a one-week inter-trial interval.

  本文關(guān)鍵詞：曠場實(shí)驗(yàn)，，由筆耕文化傳播整理發(fā)布。