重金屬作為土壤污染的主要污染因子,嚴(yán)重影響到生態(tài)安全和人體健康。利用植物營養(yǎng)元素與重金屬離子間的拮抗作用修復(fù)重金屬污染土壤,成為重金屬污染治理的一項重要技術(shù)手段。硒和鋁是已被證實(shí)的重金屬拮抗離子。本文通過盆栽試驗研究了鉬硒配施對不同鉛污染水平(0、5、50mg·kg)上小白菜鉛毒害的緩解作用。通過分析小白菜鮮重和干重、不同部位微量元素含量、抗氧化指標(biāo)以及土壤相關(guān)元素含量的變化,初步探討了鉬硒緩解鉛毒害的生理機(jī)制。主要研究結(jié)論如下： 1.低濃度的鉛(5mg-kg)對小白菜的生長代謝視乎沒有太大的影響,但是施用高濃度鉛(50mg-kg)顯著地抑制了小白菜的生長,降低了小白菜地上地下部的生物量。高濃度鉛脅迫下,硒鉬單施及配施均顯著緩解了鉛的毒害作用。施硒對正常生長條件下的小白菜產(chǎn)量有著顯著地降低作用,但是施硒能顯著地增加5mg kg-1及50mgkg-1兩個鉛水平上小白菜的產(chǎn)量。施鋁增加了正常生長條件下小白菜的產(chǎn)量,但是卻降低了50mg kg鉛水平上小白菜的產(chǎn)量。鉬硒配施的處理顯著地增加了所有鉛水平上小白菜的產(chǎn)量,甚至在50mg kg鉛水平上小白菜的產(chǎn)量也達(dá)到了顯著性水平。 2.隨著土壤鉛水...

【文章頁數(shù)】：72 頁

【學(xué)位級別】：碩士

【文章目錄】：
摘要
Abstract
Abbreviations
CHAPTER 1
    1. Introduction
        1.1 Selenium
        1.2 Molybdenum
        1.3 Brassica campestris L. ssp. Pekinensis
        1.4 Literature review
            1.4.1 Pb as a threat to the environment
            1.4.2 Selenium and Molybdenum as potential antagonists
            1.4.3 Hyperaccumulator
    2. Background and objectives of the research
        2.1 Research main objectives
        2.2 Specific Objectives
        2.3 Research structure pathway
CHAPTER2 The effects of Se and Mo on the growth of Brassica campestris L. ssp. pekinensis underPb stress
    2.1 Introduction
    2.2 Materials and methods
        2.2.1 Soil used in this study
        2.2.2 Plant Material and growth condition
        2.2.3 Experiment Treatments
        2.2.4 Plant biomass
        2.2.5 Statistical Analysis
    2.3 Results
        2.3.1 Morphology
        2.3.2 Biomass
        3.3.3 Discussions
CHAPTER3 Effect of Se and Mo on the content of Pb in Brassica campestris L. ssp. Pekinensis tissuesunder Pb stress
    3.1 Introduction
    3.2 Materials and Methods
        3.2.1 Determination of Pb Content in shoots and roots
        3.2.2 Pb assay in the soil
        3.2.3 Statistical Analysis
    3.3 Results
        3.3.1 Pb content in the shoots and roots of Brassica campestris L. ssp. pekinensis
        3.3.2 Pb concentration in the soil
    3.4 Discussion
        3.4.1 Pb content in the shoots and roots of Brassica campestris L. ssp. Pekinensis
        3.4.2 Pb concentration in the soil
CHAPTER 4 Antagonistic interaction between Se and Mo under the influence of Pb stress in Brassicacampestris L. ssp. Pekinensis
    4.1 Introduction
    4.2 Materials and Methods
        4.2.1 Soil
        4.2.2 Assay of Se content in the shoots and roots
        4.2.3 Analysis of Mo content in the leafs and roots of
        4.2.4 Assay of Se concentration in the soil after experiment
        4.2.5 Assay of Mo concentration in the soil after experiment
    4.3 Statistical Analysis
    4.4 Results
        4.4.1 Se content in the shoots and roots of Brassica campestris L. ssp. Pekinensis
        4.4.2 Mo contents in the shoots and roots of Brassica campestris L. ssp. Pekinensis
        4.4.3 Se concentration in the soil after the experiment
        4.4.4 Mo concentration in the soil after the experiment
    4.5 Discussion
        4.5.1 Se content in the shoots and roots of Brassica campestris L. ssp. Pekinensis
        4.5.2 Mo contents in the shoots and roots of Brassica campestris L. ssp. Pekinensis
        4.5.3 Se concentration in the soil after the experiment
        4.5.4 Mo concentration in the soil after the experiment
CHAPTER 5 Effect of Se and Mo on the minerals elements uptake by Brassica campestris L. ssp.Pekinensis under Pb stress
    5.1 Introduction
    5.2 Materials and methods
        5.2.1 Determination micro-elements in shoots and roots
        5.2.2 Statistical Analysis
    5.3 Results
        5.3.1 Macro and micro-elements content in shoots and roots of Brassica campestris L. ssp. Pekinensis under Pb stress
    5.4 Discussion
CHAPTER6 Reaction of antioxidant enzymes activities on Se and Mo in attempt to alleviate Pb toxicity
    6.1 Introduction
    6.2 Materials and Methods
        6.2.1 Soil utilized in this study
        6.2.2 Plant materials
        6.2.3 Analysis of antioxidant enzymes activities:SOD, CAT and POD
        6.2.4 Analysis of antioxidant non-enzymes activities:AsA and GSH
        6.2.5 Statistical Analysis
    6.3 Results
        6.3.1 Analysis of antioxidant enzymes activities (SOD EC 1.15.1.1) in shoots of Brassica campestris L. ssp. pekinensis
        6.3.2 CAT EC 1.11.1.6 enzymes activity in the shoots of Brassica campestris L. ssp.pekinensis
        6.3.3 POD EC 1.11.1.7 Enzymes activity in the shoots of Brassica campestris L. ssp.pekinensis
    6.4 Non-enzymes antioxidants contents in the shoots of Brassica campestris L. ssp.pekinensis
        6.4.1 AsA contents
        6.4.2 GSH contents
    6.5 Discussion
CHAPTER 7
    7.1 conclusion
References
Acknowledgements



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