四紋豆象的胚胎和卵巢發(fā)育特點(diǎn)與防治研究
發(fā)布時(shí)間:2019-05-22 05:01
【摘要】:四紋豆象(Callosobruchus maculatus)隸屬鞘翅目,豆象科,瘤背豆象屬,是一種世界性檢疫害蟲,在我國一些地區(qū)已發(fā)現(xiàn)該害蟲,給豆類生產(chǎn)、農(nóng)業(yè)經(jīng)濟(jì)發(fā)展造成巨大損失。研究四紋豆象的生物學(xué)習(xí)性能為口岸檢疫工作提供鑒定基礎(chǔ),胚胎發(fā)育和卵巢發(fā)育的研究能對害蟲的發(fā)生進(jìn)行預(yù)測預(yù)報(bào),為四紋豆象的綜合防治提供理論基礎(chǔ)。通過解剖四紋豆象的卵和雌成蟲,初步研究了其胚胎和卵巢發(fā)育特點(diǎn)。四紋豆象胚胎發(fā)育經(jīng)歷卵裂期、胚盤胚帶形成期、胚層胚膜形成期、胚體分節(jié)和附肢形成期、胚胎背合與胚胎成熟階段等5個(gè)階段,雌成蟲在溫度28℃,相對濕度70%條件下,胚胎發(fā)育歷期為118h,其中產(chǎn)卵后O-11h為卵裂期,12h-16h為胚盤、胚帶形成期,17h-20h為胚層胚膜形成期、21h-90h為胚體分節(jié)和附肢形成階段,92h-118h是胚胎背合與胚胎成熟階段。同樣溫濕度條件下,卵巢發(fā)育分為卵黃沉積期、卵粒成熟期、成熟待產(chǎn)期、產(chǎn)卵盛期和產(chǎn)卵末期5個(gè)級別,雌蟲產(chǎn)卵歷期為15天左右。在初步探討防治方法上面,用微波加熱處理120s和-25℃冷凍條件下處理0.5h以及50℃以上熱水侵泡0.5h均能使四紋豆象各蟲態(tài)死亡率達(dá)到100%,使用5個(gè)濃度梯度的除蟲菊素、阿維菌素、苦參堿、蘇云金桿菌處理四紋豆象成蟲,統(tǒng)計(jì)72h的死亡率,得出LC5o分別為7.13576ug/ml、3.83465ug/ml、72.47061ug/ml、41.71291ug/ml,其中阿維菌素的毒性最強(qiáng),除蟲菊素次之。
[Abstract]:(Callosobruchus maculatus) belongs to Coleoptera, Leguminosae and Elaeagnus. It is a worldwide quarantine pest. It has been found in some areas of China, which has caused great losses to legume production and agricultural economic development. The study on the biological learning performance of the four striped bean elephant provides the identification basis for the port quarantine work. The study of embryo development and ovarian development can predict the occurrence of pests and provide a theoretical basis for the comprehensive control of the four striped bean elephant. The embryonic and ovarian development characteristics of Euphorbia pulcherrima were studied by dissecting the eggs and female adults of Elaeagnus angustifolia. The embryo development of Elaeagnus angustifolia experienced five stages: cleavage stage, embryo disk embryo band formation stage, embryo layer embryo membrane formation stage, embryo segmented and appendage formation stage, embryo dorsal integration and embryo maturation stage. The female adults were at 28 鈩,
本文編號:2482697
[Abstract]:(Callosobruchus maculatus) belongs to Coleoptera, Leguminosae and Elaeagnus. It is a worldwide quarantine pest. It has been found in some areas of China, which has caused great losses to legume production and agricultural economic development. The study on the biological learning performance of the four striped bean elephant provides the identification basis for the port quarantine work. The study of embryo development and ovarian development can predict the occurrence of pests and provide a theoretical basis for the comprehensive control of the four striped bean elephant. The embryonic and ovarian development characteristics of Euphorbia pulcherrima were studied by dissecting the eggs and female adults of Elaeagnus angustifolia. The embryo development of Elaeagnus angustifolia experienced five stages: cleavage stage, embryo disk embryo band formation stage, embryo layer embryo membrane formation stage, embryo segmented and appendage formation stage, embryo dorsal integration and embryo maturation stage. The female adults were at 28 鈩,
本文編號:2482697
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