馬鈴薯塊莖形成期糖類物質(zhì)代謝機(jī)制與產(chǎn)量形成的研究
發(fā)布時(shí)間：2019-05-12 00:38
【摘要】：測定馬鈴薯塊莖形成期(出苗后40~50d)、膨大期(出苗后50~70d)、淀粉積累期(出苗后70~80d)、成熟期(出苗后80~90d)葉片、葉柄、根系、匍匐莖和塊莖糖類物質(zhì)含量及糖代謝相關(guān)酶活性與產(chǎn)量形成,研究馬鈴薯塊莖生長期間不同器官和塊莖不同部位糖類物質(zhì)含量及糖代謝相關(guān)酶活性的變化動態(tài)、塊莖生育期內(nèi)淀粉含量與其它糖類物質(zhì)的關(guān)系、馬鈴薯產(chǎn)量的變化動態(tài),形成馬鈴薯糖分積累規(guī)律,明確糖類物質(zhì)代謝對馬鈴薯產(chǎn)量形成的作用機(jī)制,為馬鈴薯的產(chǎn)量提高和生理研究提供技術(shù)指導(dǎo)和理論依據(jù)。主要試驗(yàn)結(jié)果如下:1.出苗后40~50d,塊莖淀粉葡萄糖和果糖含量升高,蔗糖、可溶性糖和還原糖含量降低。葉片淀粉、蔗糖、可溶性糖、還原糖、葡萄糖和果糖含量升高。葉柄和根系可溶性糖、還原糖、葡萄糖和果糖含量升高,淀粉和蔗糖含量降低。匍匐莖蔗糖、還原糖、葡萄糖和果糖含量升高,淀粉和可溶性糖含量降低。出苗后50~60d,塊莖淀粉和果糖含量升高,蔗糖、可溶性糖、還原糖和葡萄糖含量降低。葉片葡萄糖和果糖含量升高,淀粉、蔗糖、還原糖和可溶性糖含量降低。葉柄淀粉、蔗糖、可溶性糖、還原糖、葡萄糖和果糖含量降低。根系淀粉含量升高,蔗糖、可溶性糖、還原糖、葡萄糖和果糖含量降低。匍匐莖淀粉和可溶性糖含量升高,蔗糖、還原糖、葡萄糖和果糖含量降低。出苗后60~70d,塊莖淀粉和果糖含量升高,蔗糖、可溶性糖、還原糖和葡萄糖含量降低。葉片葡萄糖和果糖含量升高,淀粉、蔗糖、還原糖和可溶性糖含量降低。葉柄淀粉、蔗糖、可溶性糖、還原糖、葡萄糖和果糖含量降低。根系淀粉含量升高,蔗糖、可溶性糖、還原糖、葡萄糖和果糖含量降低。匍匐莖淀粉和可溶性糖含量升高,蔗糖、還原糖、葡萄糖和果糖含量降低。出苗后70~80d,塊莖淀粉含量升高,蔗糖、可溶性糖、還原糖、葡萄糖和果糖含量降低。葉片和葉柄蔗糖、還原糖、可溶性糖、葡萄糖和果糖含量升高,淀粉含量降低。根系蔗糖和果糖含量升高,淀粉、可溶性糖、還原糖和葡萄糖含量降低。匍匐莖蔗糖、還原糖、葡萄糖和果糖含量升高,淀粉和可溶性糖含量降低。出苗后80~90d,塊莖淀粉、蔗糖、可溶性糖和果糖含量升高,還原糖和葡萄糖含量降低。2.出苗后40~50d,塊莖SS、SPS、淀粉磷酸化酶和淀粉酶活性升高,轉(zhuǎn)化酶活性降低。葉片轉(zhuǎn)化酶、SS和SPS活性升高。葉柄轉(zhuǎn)化酶和SS活性升高,SPS活性降低。根系轉(zhuǎn)化酶活性升高,SS和SPS活性降低。匍匐莖SPS活性升高,轉(zhuǎn)化酶和SS活性降低。出苗后50~60d,塊莖SS和淀粉磷酸化酶活性升高,轉(zhuǎn)化酶、SPS和淀粉酶活性降低。葉片和葉柄轉(zhuǎn)化酶、SS和SPS活性降低。根系SS活性升高,轉(zhuǎn)化酶和SPS活性降低。匍匐莖轉(zhuǎn)化酶和SS活性升高,SPS活性降低。出苗后60~70d,塊莖SS、SPS和淀粉磷酸化酶活性升高,轉(zhuǎn)化酶和淀粉酶活性降低。葉片和葉柄轉(zhuǎn)化酶、SS和SPS活性降低。根系SS活性升高,轉(zhuǎn)化酶和SPS活性降低。匍匐莖轉(zhuǎn)化酶和SS活性升高,SPS活性降低。出苗后70~80d,塊莖SS活性升高,轉(zhuǎn)化酶、SPS、淀粉磷酸化酶和淀粉酶活性降低。葉片轉(zhuǎn)化酶和SPS活性升高,SS活性降低。葉柄、根系和匍匐莖SPS活性升高,轉(zhuǎn)化酶和SS活性降低。出苗后80~90d,塊莖SS活性升高,轉(zhuǎn)化酶、SPS、淀粉磷酸化酶和淀粉酶活性降低。3.通過多元回歸分析和通徑分析,葉片還原糖和葉柄蔗糖對塊莖淀粉形成積累具有促進(jìn)作用,其中葉柄蔗糖作用更大。4.通過多元回歸分析和通徑分析,葉柄SPS和塊莖淀粉磷酸化酶對塊莖淀粉形成積累具有促進(jìn)作用,其中葉柄SPS作用更大,通過促進(jìn)葉柄蔗糖形成,不斷運(yùn)輸至塊莖進(jìn)而促進(jìn)塊莖淀粉形成和積累。5.糖類物質(zhì)含量在塊莖各部位間有差異,但不同部位隨著生育期的推移淀粉含量升高,淀粉含量髓部最高;蔗糖含量降低,蔗糖含量髓部最高;還原糖和葡萄糖含量變化呈單峰曲線,出苗后70d達(dá)峰值;可溶性糖與果糖含量變化相反。塊莖不同部位轉(zhuǎn)化酶和蔗糖合成酶僅在出苗后70d達(dá)波谷;蔗糖磷酸合成酶和淀粉磷酸化酶呈單峰曲線,出苗后70d達(dá)峰值;淀粉酶活性不斷降低。6.通過多元回歸分析和通徑分析,葉片和葉柄葡萄糖、匍匐莖可溶性糖含量對馬鈴薯產(chǎn)量形成作用效果顯著。
[Abstract]:the content of the leaf, the petiole, the root system, the stolon and the content of the carbohydrate in the leaves, the petiole, the root system, the stolon and the tuber of the stem and the tuber of the stem and the tuber of the root, the stolon and the tuber of the tuber of the potato are determined, and the activity and the yield of the sugar metabolism-related enzyme are determined, The changes of the content of carbohydrate and the activity of sugar metabolism in different parts of the potato tuber during the growth of the potato tuber were studied. The relationship between the content of starch and other carbohydrate substances in the growth period of the tuber and the change of the yield of the potato were studied, and the accumulation of sugar in the potato was formed. The mechanism of the effect of carbohydrate metabolism on the yield of potato was clarified, which provided technical guidance and theoretical basis for the improvement of the yield and the physiological research of the potato. The main test results are as follows:1. The content of sucrose, soluble sugar and reducing sugar decreased 40-50 days after emergence, and the content of sugar, soluble sugar and reducing sugar of tuber starch decreased. Leaf starch, sucrose, soluble sugar, reducing sugar, glucose and fructose content increased. The content of soluble sugar, reducing sugar, glucose and fructose in petiole and root system increased, and the content of starch and sucrose decreased. The content of sucrose, reducing sugar, glucose and fructose increased, and the content of starch and soluble sugar decreased. The content of sucrose, soluble sugar, reducing sugar and glucose decreased from 50 to 60 days after emergence. The content of glucose and fructose in the leaves is increased, and the content of starch, sucrose, reducing sugar and soluble sugar is reduced. The content of petiole starch, sucrose, soluble sugar, reducing sugar, glucose and fructose is reduced. The starch content of root system was increased, and the content of sucrose, soluble sugar, reducing sugar, glucose and fructose was reduced. The content of stolon and soluble sugar increased, and the content of sucrose, reducing sugar, glucose and fructose decreased. The content of sucrose, soluble sugar, reducing sugar and glucose decreased from 60 to 70 days after emergence. The content of glucose and fructose in the leaves is increased, and the content of starch, sucrose, reducing sugar and soluble sugar is reduced. The content of petiole starch, sucrose, soluble sugar, reducing sugar, glucose and fructose is reduced. The starch content of root system was increased, and the content of sucrose, soluble sugar, reducing sugar, glucose and fructose was reduced. The content of stolon and soluble sugar increased, and the content of sucrose, reducing sugar, glucose and fructose decreased. The content of sucrose, soluble sugar, reducing sugar, glucose and fructose decreased from 70 to 80 days after emergence. The content of sucrose, reducing sugar, soluble sugar, glucose and fructose in leaves and petioles increased and the content of starch decreased. The contents of sucrose and fructose in root system increased, and the content of starch, soluble sugar, reducing sugar and glucose decreased. The content of sucrose, reducing sugar, glucose and fructose increased, and the content of starch and soluble sugar decreased. 80 to 90 days after emergence, the content of starch, sucrose, soluble sugar and fructose increased, and the content of reducing sugar and glucose decreased. 40 to 50 days after the emergence of the seedling, the activity of the tuber SS, the SPS, the starch phosphorylase and the amylase increased, and the activity of the invertase was reduced. The activity of invertase, SS and SPS increased. The activity of the petiole invertase and SS increased and the SPS activity decreased. The activity of invertase in root system was increased, and the activity of SS and SPS was decreased. The activity of SPS and invertase and SS of stolon were decreased. 50-60 days after emergence, the activity of tuber SS and starch phosphorylase increased, and the activity of invertase, SPS and amylase decreased. Leaf and petiole invertase, SS and SPS activity decreased. The activity of the root SS increased, the invertase and SPS activity decreased. The activity of invertase and SS increased and the activity of SPS decreased. 60 to 70 days after the emergence of the seedling, the activity of the tuber SS, the SPS and the starch phosphorylase increased, and the activity of the invertase and the amylase was reduced. Leaf and petiole invertase, SS and SPS activity decreased. The activity of the root SS increased, the invertase and SPS activity decreased. The activity of invertase and SS increased and the activity of SPS decreased. The activity of SS, invertase, SPS, starch phosphorylase and amylase decreased from 70 to 80 days after emergence. The activity of invertase and SPS increased and the activity of SS decreased. The activity of the petioles, roots and stolons increased, and the activity of invertase and SS decreased. The activity of the tuber SS, the invertase, the SPS, the starch phosphorylase and the amylase activity decreased from 80 to 90 days after the emergence of the seedlings. Through multiple regression analysis and path analysis, the effect of leaf reducing sugar and petiole sucrose on the formation of tuber starch was promoted, and the effect of petiole sucrose was greater. The effect of petiole SPS and tuber starch phosphorylase on the formation and accumulation of tuber starch was promoted by multiple regression analysis and path analysis, and the effect of petiole SPS was greater, and the formation and accumulation of tuber starch was promoted by promoting the formation of petiole sucrose. The content of carbohydrate was different among the various parts of the tuber, but the content of the starch increased with the increasing of the growth period, the content of the content of the starch was the highest, the content of the sucrose was lower, the content of the content of the sucrose was the highest, the change of the reducing sugar and the content of the glucose was a unimodal curve, and the peak of 70d after the emergence of the seedling was reached. The content of soluble sugar is opposite to that of fructose. The invertase and the sucrose synthase in different parts of the tuber only reach the trough after the emergence of the seedling; the sucrose-phosphate synthase and the starch phosphorylase show a single-peak curve, and the peak value is 70d after the emergence of the seedling; and the activity of the amylase is continuously reduced. Through the multiple regression analysis and path analysis, the soluble sugar content of the leaves and petioles of the leaf and the petiole of the petiole has a remarkable effect on the yield of the potato.
【學(xué)位授予單位】：黑龍江八一農(nóng)墾大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：S532
[Abstract]:the content of the leaf, the petiole, the root system, the stolon and the content of the carbohydrate in the leaves, the petiole, the root system, the stolon and the tuber of the stem and the tuber of the stem and the tuber of the root, the stolon and the tuber of the tuber of the potato are determined, and the activity and the yield of the sugar metabolism-related enzyme are determined, The changes of the content of carbohydrate and the activity of sugar metabolism in different parts of the potato tuber during the growth of the potato tuber were studied. The relationship between the content of starch and other carbohydrate substances in the growth period of the tuber and the change of the yield of the potato were studied, and the accumulation of sugar in the potato was formed. The mechanism of the effect of carbohydrate metabolism on the yield of potato was clarified, which provided technical guidance and theoretical basis for the improvement of the yield and the physiological research of the potato. The main test results are as follows:1. The content of sucrose, soluble sugar and reducing sugar decreased 40-50 days after emergence, and the content of sugar, soluble sugar and reducing sugar of tuber starch decreased. Leaf starch, sucrose, soluble sugar, reducing sugar, glucose and fructose content increased. The content of soluble sugar, reducing sugar, glucose and fructose in petiole and root system increased, and the content of starch and sucrose decreased. The content of sucrose, reducing sugar, glucose and fructose increased, and the content of starch and soluble sugar decreased. The content of sucrose, soluble sugar, reducing sugar and glucose decreased from 50 to 60 days after emergence. The content of glucose and fructose in the leaves is increased, and the content of starch, sucrose, reducing sugar and soluble sugar is reduced. The content of petiole starch, sucrose, soluble sugar, reducing sugar, glucose and fructose is reduced. The starch content of root system was increased, and the content of sucrose, soluble sugar, reducing sugar, glucose and fructose was reduced. The content of stolon and soluble sugar increased, and the content of sucrose, reducing sugar, glucose and fructose decreased. The content of sucrose, soluble sugar, reducing sugar and glucose decreased from 60 to 70 days after emergence. The content of glucose and fructose in the leaves is increased, and the content of starch, sucrose, reducing sugar and soluble sugar is reduced. The content of petiole starch, sucrose, soluble sugar, reducing sugar, glucose and fructose is reduced. The starch content of root system was increased, and the content of sucrose, soluble sugar, reducing sugar, glucose and fructose was reduced. The content of stolon and soluble sugar increased, and the content of sucrose, reducing sugar, glucose and fructose decreased. The content of sucrose, soluble sugar, reducing sugar, glucose and fructose decreased from 70 to 80 days after emergence. The content of sucrose, reducing sugar, soluble sugar, glucose and fructose in leaves and petioles increased and the content of starch decreased. The contents of sucrose and fructose in root system increased, and the content of starch, soluble sugar, reducing sugar and glucose decreased. The content of sucrose, reducing sugar, glucose and fructose increased, and the content of starch and soluble sugar decreased. 80 to 90 days after emergence, the content of starch, sucrose, soluble sugar and fructose increased, and the content of reducing sugar and glucose decreased. 40 to 50 days after the emergence of the seedling, the activity of the tuber SS, the SPS, the starch phosphorylase and the amylase increased, and the activity of the invertase was reduced. The activity of invertase, SS and SPS increased. The activity of the petiole invertase and SS increased and the SPS activity decreased. The activity of invertase in root system was increased, and the activity of SS and SPS was decreased. The activity of SPS and invertase and SS of stolon were decreased. 50-60 days after emergence, the activity of tuber SS and starch phosphorylase increased, and the activity of invertase, SPS and amylase decreased. Leaf and petiole invertase, SS and SPS activity decreased. The activity of the root SS increased, the invertase and SPS activity decreased. The activity of invertase and SS increased and the activity of SPS decreased. 60 to 70 days after the emergence of the seedling, the activity of the tuber SS, the SPS and the starch phosphorylase increased, and the activity of the invertase and the amylase was reduced. Leaf and petiole invertase, SS and SPS activity decreased. The activity of the root SS increased, the invertase and SPS activity decreased. The activity of invertase and SS increased and the activity of SPS decreased. The activity of SS, invertase, SPS, starch phosphorylase and amylase decreased from 70 to 80 days after emergence. The activity of invertase and SPS increased and the activity of SS decreased. The activity of the petioles, roots and stolons increased, and the activity of invertase and SS decreased. The activity of the tuber SS, the invertase, the SPS, the starch phosphorylase and the amylase activity decreased from 80 to 90 days after the emergence of the seedlings. Through multiple regression analysis and path analysis, the effect of leaf reducing sugar and petiole sucrose on the formation of tuber starch was promoted, and the effect of petiole sucrose was greater. The effect of petiole SPS and tuber starch phosphorylase on the formation and accumulation of tuber starch was promoted by multiple regression analysis and path analysis, and the effect of petiole SPS was greater, and the formation and accumulation of tuber starch was promoted by promoting the formation of petiole sucrose. The content of carbohydrate was different among the various parts of the tuber, but the content of the starch increased with the increasing of the growth period, the content of the content of the starch was the highest, the content of the sucrose was lower, the content of the content of the sucrose was the highest, the change of the reducing sugar and the content of the glucose was a unimodal curve, and the peak of 70d after the emergence of the seedling was reached. The content of soluble sugar is opposite to that of fructose. The invertase and the sucrose synthase in different parts of the tuber only reach the trough after the emergence of the seedling; the sucrose-phosphate synthase and the starch phosphorylase show a single-peak curve, and the peak value is 70d after the emergence of the seedling; and the activity of the amylase is continuously reduced. Through the multiple regression analysis and path analysis, the soluble sugar content of the leaves and petioles of the leaf and the petiole of the petiole has a remarkable effect on the yield of the potato.
【學(xué)位授予單位】：黑龍江八一農(nóng)墾大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：S532
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10 張小靜;程李香;林必博;王蒂;張峰;;馬鈴薯塊莖蛋白質(zhì)提取方法對雙向電泳結(jié)果的影響[A];中國植物學(xué)會七十五周年年會論文摘要匯編（1933-2008）[C];2008年
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