獺兔飼糧銅適宜添加量及對脂肪代謝的影響
發(fā)布時間：2019-04-20 19:33
【摘要】：本試驗研究了斷奶-3月齡生長獺兔飼糧銅適宜添加量及銅對脂肪代謝的影響。試驗選取200只體況相近的斷奶健康獺兔,隨機(jī)分為5組,每組40個重復(fù),每個重復(fù)一只,分別飼喂銅添加水平為0、5、15、45、135 mg/kg的飼糧,預(yù)試期7d,正試期59 d。試驗結(jié)果如下:(1)飼糧不同銅水平對斷奶-3月齡生長獺兔平均日增重、平均日采食量(P0.05)有顯著影響,且當(dāng)飼糧中銅添加量為15 mg/kg時,平均日增重及平均日采食量均達(dá)到最高值。飼糧不同銅水平對斷奶-3月齡生長獺兔毛皮重量(P0.05)有顯著影響。飼糧不同銅水平對斷奶-3月齡生長獺兔肉質(zhì)滴水損失、pH值(P0.05)有顯著影響,綜合來看,銅添加量為15和45 mg/kg時,肉品質(zhì)都表現(xiàn)出較好的性狀。飼糧不同銅水平對斷奶-3月齡生長獺兔食入氮、糞氮、尿氮、沉積氮、氮的表觀消化率、氮利用率、氮生物利用率(P0.05)有顯著影響,均呈現(xiàn)出先增高后降低的趨勢。飼糧不同銅水平對斷奶-3月齡生長獺兔肝臟指數(shù)(P0.05)極顯著影響。飼糧不同銅水平對斷奶-3月齡生長獺兔血清中總蛋白、白蛋白、尿素(P0.05)有顯著影響,均呈現(xiàn)出先增高后降低的趨勢。飼糧不同銅水平可以顯著提高斷奶-3月齡生長獺兔食入銅、糞銅、可消化銅、銅的表觀消化率(P0.05)。飼糧不同銅水平對斷奶-3月齡生長獺兔肝臟銅含量(P0.05)有顯著影響,沉積在肝臟中的銅含量會隨著銅添加量的增加而增高。飼糧不同銅水平對斷奶-3月齡生長獺兔前腿肉重、后腿肉重、腿肌總重、前腿肌率、后腿肌率、腿肌總率(P0.05)有顯著影響,且當(dāng)飼糧中銅添加量為45 mg/kg時達(dá)到最大值。綜合以上指標(biāo),斷奶-3月齡生長獺兔飼糧中銅的適宜添加量為15-45 mg/kg。(2)根據(jù)以上指標(biāo),我們選取飼糧銅添加水平為0 mg/kg的試驗組作為對照組,飼糧銅添加水平為45 mg/kg的試驗組作為銅處理組,借此探討銅對生長獺兔脂肪代謝的影響機(jī)制。銅處理組可以顯著降低斷奶-3月齡生長獺兔肩部脂肪、腎周脂肪以及總脂肪的沉積(P0.05)。銅處理組顯著抑制肝臟、骨骼肌、脂肪組織中細(xì)胞質(zhì)脂質(zhì)的積累(P0.05)。與對照組相比,銅處理組顯著降低生長獺兔血清中極低密度脂蛋白的水平。銅處理組顯著增加了肝臟中CPT1、CPT2、PPARα基因的表達(dá)(P0.05)。銅處理組還可以顯著上調(diào)了骨骼肌組織中的CPT1、CPT2、LPL、PPARα、FABP、FATP m RNA的表達(dá)量和p-AMPK蛋白水平(P0.05)。銅處理組中獺兔脂肪組織中CPT1、CPT2、PPARα和HSL mRNA的表達(dá)量也比較高(P0.05)。綜合以上指標(biāo),飼糧添加銅可以促進(jìn)骨骼肌的生長并且減少脂肪沉積。銅對脂肪代謝的調(diào)節(jié)是通過肝臟、骨骼肌和脂肪組織中的PPAR信號傳導(dǎo)和骨骼肌組織中的AMPK信號傳導(dǎo)。
[Abstract]:The effects of dietary copper on fat metabolism of weanling-3-month-old Rex rabbits were studied. Two hundred weaned healthy rex rabbits in similar condition were randomly divided into 5 groups with 40 replicates in each group, each of which was fed with a diet containing 0,5,15,45135 mg/kg of copper, respectively. The trial period was 7 d, and the positive trial period was 59 d. The results were as follows: (1) the average daily gain and average daily intake of weanling-3-month-old Rex rabbits were significantly affected by different dietary copper levels (P0.05), and when the dietary copper level was 15 mg/kg, the average daily gain and average daily intake of weanling-3-month-old Rex rabbits were significantly affected (P0.05). The average daily gain and average daily intake reached the highest value. Different dietary copper levels significantly affected the fur weight of weanling-3-month-old Rex rabbits (P0.05). Different dietary copper levels had significant effects on the loss of meat quality and pH (P0.05) in weanling-3-month-old Rex rabbits. On the whole, the meat quality of weanling-3-month-old Rex rabbits showed better quality when copper was added at 15 and 45 mg/kg. Different dietary copper levels had significant effects on the apparent digestibility of nitrogen, fecal nitrogen, urine nitrogen, deposited nitrogen, nitrogen bioavailability (P0.05) in weanling-3-month-old Rex rabbits, all of which showed a tendency of increasing first and then decreasing. The liver index of weanling-3-month-old Rex rabbits was significantly affected by different dietary copper levels (P0.05). Dietary copper levels had significant effects on serum total protein, albumin and urea (P0.05) in weanling-3-month-old Rex rabbits, all of which showed a tendency of increasing first and then decreasing. Dietary copper levels significantly increased the apparent digestibility of copper, fecal copper, digestible copper and copper in weanling-3-month-old Rex rabbits (P0.05). The content of copper in liver of weanling-3-month-old Rex rabbits was significantly affected by different dietary copper levels (P0.05). The copper content deposited in liver increased with the increase of copper content. Different dietary copper levels significantly affected the foreleg meat weight, hind leg meat weight, total leg muscle weight, foreleg muscle rate, hind leg muscle rate and leg muscle total rate (P0.05) in weanling-3 month-old Rex rabbits. And when the dietary copper content was 45 mg/kg, the maximum value was reached. According to the above indexes, the appropriate addition of copper in diets of weanling-3-month-old Rex rabbits was 15? 45 mg/kg. (2) according to the above indexes, we selected the trial group with 0 mg/kg level of dietary copper as the control group. The effects of copper on fat metabolism in growing Rex rabbits were studied by adding 45 mg/kg of copper to the experimental group as the copper treatment group, and the effects of copper on fat metabolism of growing Rex rabbits were studied. Copper treatment could significantly reduce the deposition of shoulder fat, perirenal fat and total fat in weanling-3-month-old Rex rabbits (P0.05). Copper treatment significantly inhibited the accumulation of cytoplasmic lipid in liver, skeletal muscle and adipose tissue (P0.05). Compared with the control group, the copper treatment group significantly decreased the level of very low density lipoprotein (VLDL) in the serum of growing Rex rabbits. Copper treatment significantly increased the expression of CPT1,CPT2,PPAR 偽 gene in liver (P0.05). Copper treatment could also significantly up-regulate the expression of CPT1,CPT2,LPL,PPAR 偽, FABP,FATP m RNA and p-AMPK protein in skeletal muscle (P0.05). The expression levels of CPT1,CPT2, PPAR偽 and HSL mRNA in adipose tissue of rex rabbits were also higher in copper treated group (P0.05). Combined with the above indicators, dietary copper supplementation can promote skeletal muscle growth and reduce fat deposition. Copper regulates adipose metabolism through PPAR signal transduction in liver, skeletal muscle and adipose tissue and AMPK signal transduction in skeletal muscle tissue.
【學(xué)位授予單位】：山東農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：S829.1
[Abstract]:The effects of dietary copper on fat metabolism of weanling-3-month-old Rex rabbits were studied. Two hundred weaned healthy rex rabbits in similar condition were randomly divided into 5 groups with 40 replicates in each group, each of which was fed with a diet containing 0,5,15,45135 mg/kg of copper, respectively. The trial period was 7 d, and the positive trial period was 59 d. The results were as follows: (1) the average daily gain and average daily intake of weanling-3-month-old Rex rabbits were significantly affected by different dietary copper levels (P0.05), and when the dietary copper level was 15 mg/kg, the average daily gain and average daily intake of weanling-3-month-old Rex rabbits were significantly affected (P0.05). The average daily gain and average daily intake reached the highest value. Different dietary copper levels significantly affected the fur weight of weanling-3-month-old Rex rabbits (P0.05). Different dietary copper levels had significant effects on the loss of meat quality and pH (P0.05) in weanling-3-month-old Rex rabbits. On the whole, the meat quality of weanling-3-month-old Rex rabbits showed better quality when copper was added at 15 and 45 mg/kg. Different dietary copper levels had significant effects on the apparent digestibility of nitrogen, fecal nitrogen, urine nitrogen, deposited nitrogen, nitrogen bioavailability (P0.05) in weanling-3-month-old Rex rabbits, all of which showed a tendency of increasing first and then decreasing. The liver index of weanling-3-month-old Rex rabbits was significantly affected by different dietary copper levels (P0.05). Dietary copper levels had significant effects on serum total protein, albumin and urea (P0.05) in weanling-3-month-old Rex rabbits, all of which showed a tendency of increasing first and then decreasing. Dietary copper levels significantly increased the apparent digestibility of copper, fecal copper, digestible copper and copper in weanling-3-month-old Rex rabbits (P0.05). The content of copper in liver of weanling-3-month-old Rex rabbits was significantly affected by different dietary copper levels (P0.05). The copper content deposited in liver increased with the increase of copper content. Different dietary copper levels significantly affected the foreleg meat weight, hind leg meat weight, total leg muscle weight, foreleg muscle rate, hind leg muscle rate and leg muscle total rate (P0.05) in weanling-3 month-old Rex rabbits. And when the dietary copper content was 45 mg/kg, the maximum value was reached. According to the above indexes, the appropriate addition of copper in diets of weanling-3-month-old Rex rabbits was 15? 45 mg/kg. (2) according to the above indexes, we selected the trial group with 0 mg/kg level of dietary copper as the control group. The effects of copper on fat metabolism in growing Rex rabbits were studied by adding 45 mg/kg of copper to the experimental group as the copper treatment group, and the effects of copper on fat metabolism of growing Rex rabbits were studied. Copper treatment could significantly reduce the deposition of shoulder fat, perirenal fat and total fat in weanling-3-month-old Rex rabbits (P0.05). Copper treatment significantly inhibited the accumulation of cytoplasmic lipid in liver, skeletal muscle and adipose tissue (P0.05). Compared with the control group, the copper treatment group significantly decreased the level of very low density lipoprotein (VLDL) in the serum of growing Rex rabbits. Copper treatment significantly increased the expression of CPT1,CPT2,PPAR 偽 gene in liver (P0.05). Copper treatment could also significantly up-regulate the expression of CPT1,CPT2,LPL,PPAR 偽, FABP,FATP m RNA and p-AMPK protein in skeletal muscle (P0.05). The expression levels of CPT1,CPT2, PPAR偽 and HSL mRNA in adipose tissue of rex rabbits were also higher in copper treated group (P0.05). Combined with the above indicators, dietary copper supplementation can promote skeletal muscle growth and reduce fat deposition. Copper regulates adipose metabolism through PPAR signal transduction in liver, skeletal muscle and adipose tissue and AMPK signal transduction in skeletal muscle tissue.
【學(xué)位授予單位】：山東農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：S829.1
【參考文獻(xiàn)】
相關(guān)期刊論文 前10條
1 韓建;彭維兵;韓利文;何秋霞;張云;孫晨;王榮春;楚杰;劉可春;;銅、汞和鉛對斑馬魚仔魚肝臟發(fā)育的影響[J];山東科學(xué);2016年05期
2 吳學(xué)壯;劉志;郭俊剛;崔虎;萬春孟;王忠成;高秀華;楊福合;邢秀梅;;飼糧銅、鋅含量及其互作效應(yīng)對育成期水貂生長性能、營養(yǎng)物質(zhì)表觀消化率及銅、鋅和氮代謝的影響[J];動物營養(yǎng)學(xué)報;2015年08期
3 徐晨晨;王寶維;葛文華;張名愛;岳斌;史雪萍;;銅對5-16周齡五龍鵝生長性能、屠宰性能、營養(yǎng)物質(zhì)利用率和血清激素含量的影響[J];動物營養(yǎng)學(xué)報;2013年09期
4 肖飛;王朝旭;楊麗;;銅缺乏對大鼠鐵代謝的影響[J];衛(wèi)生研究;2013年04期
5 趙明夫;;肉質(zhì)評價的指標(biāo)[J];養(yǎng)殖技術(shù)顧問;2012年05期
6 呼顯生;黃克群;姜成;李沐森;;羔羊慢性銅中毒的病理學(xué)診斷與治療[J];畜牧與獸醫(yī);2011年06期
7 李金博;李愛華;巫亮;陳志強(qiáng);張蕊;楊麗娟;;灘母羊日糧中銅水平對其羔羊裘皮品質(zhì)影響的研究[J];黑龍江畜牧獸醫(yī);2010年15期
8 徐之勇;朱奎成;崔恒敏;彭西;鄧俊良;吳強(qiáng);楊帆;趙麗;;日糧高銅對雛雞血清免疫球蛋白含量的影響[J];中國獸醫(yī)學(xué)報;2008年11期
9 蓋文婷;朱連勤;朱風(fēng)華;孫金全;董菊紅;;納米氧化銅對小白鼠銅表觀消化率的影響[J];家畜生態(tài)學(xué)報;2008年05期
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