本文選題：卵泡囊腫 + 雌激素受體��； 參考：《吉林大學(xué)》2017年碩士論文

【摘要】：卵泡囊腫是母豬常見的繁殖障礙性疾病之一,會導(dǎo)致母豬不孕。它會引起母畜持續(xù)、頻繁、短暫和無規(guī)律的發(fā)情,該病對豬飼養(yǎng)產(chǎn)業(yè)的發(fā)展造成了巨大的阻礙�，F(xiàn)階段,隨著分子生物學(xué)的研究愈加深入,尋找豬卵泡囊腫標(biāo)記基因的研究也越來越受重視。ESR、FSHβ和Leptin基因均可以通過調(diào)控下丘腦-垂體-卵巢軸的每個環(huán)節(jié)從而對母豬卵巢生長發(fā)育起到重要的作用。ESR(雌激素受體)是類固醇受體家族的成員之一,它能夠調(diào)控雌性哺乳動物的生殖活動,影響雌性動物性腺組織基因的表達(dá)和調(diào)控,進一步影響動物個體的第二性征,繁殖周期,生殖力,妊娠維持等。有研究結(jié)果表明豬ESR基因PvuⅡ多態(tài)性酶切位點對豬的產(chǎn)仔數(shù)和繁殖性狀有顯著影響。然而目前尚無關(guān)于分析該位點與卵泡囊腫關(guān)系的報道。FSH(卵泡刺激素)是一種糖蛋白類促性腺激素,對促進顆粒細(xì)胞成熟有重要的作用。豬的FSH基因結(jié)構(gòu)包含一長度為292bp的逆轉(zhuǎn)座子,由于逆轉(zhuǎn)座子的插入而導(dǎo)致了該基因位點存在多態(tài)性。經(jīng)過對該位點的分析發(fā)現(xiàn),FSHβ基因是影響豬繁殖性狀的主效基因。但是目前尚無研究把FSHβ基因作為侯選基因來分析其與卵泡囊腫的關(guān)系。Leptin(瘦素)是由脂肪細(xì)胞分泌的一類蛋白質(zhì)類激素,其基因表達(dá)產(chǎn)物主要參與動物生長發(fā)育和繁殖功能等方面的調(diào)節(jié)。有研究表明,豬leptin基因第3外顯子T3469C位點變異對豬的繁殖性能具有一定影響。然而,尚無研究將Leptin基因作為侯選基因來分析其與卵泡囊腫的關(guān)系。本實驗以雌激素受體(ESR)、促卵泡素β亞基(FSHβ)和瘦素(Leptin)作為研究對象。收集健康卵巢和患病卵巢后,通過提取卵巢基因組DNA,然后采用直接電泳法和PCR-RFLP法對雌激素受體(ESR)、促卵泡素β亞基(FSHβ)和瘦素(Leptin)進行基因多態(tài)位點檢測,并進一步分析該位點與卵泡囊腫的關(guān)系,尋找與卵泡囊腫有關(guān)的分子標(biāo)記。主要內(nèi)容如下:(1)基因多態(tài)性檢測結(jié)果顯示:3個基因共檢測到4個多態(tài)位點,分別是位于ESR基因內(nèi)含子AG突變;位于FSHβ基因內(nèi)含子5’調(diào)控區(qū)TC突變;位于FSHβ基因內(nèi)含子CT突變;位于leptin基因外顯子TC突變。(2)多態(tài)信息含量、雜合度和有效等位基因分析結(jié)果顯示:ESR PvuⅡ多態(tài)酶切位點在兩群體中均呈現(xiàn)中度多態(tài)(0.25PIC0.5),該多態(tài)位點基因型分布在囊腫群體中背離Hardy-Weinberg平衡狀態(tài)(P0.05),在健康豬群體中符合Hardy-Weinberg平衡(P0.05);FSHβ5’調(diào)控區(qū)多態(tài)位點在卵泡囊腫豬群體中呈現(xiàn)低度多態(tài)(PIC0.25),健康豬群體中均呈現(xiàn)中度多態(tài)(0.25PIC0.5)。該多態(tài)位點基因型分布兩群體中均背離Hardy-Weinberg平衡狀態(tài)(P0.05);FSHβ-HaeⅢ多態(tài)酶切位點在兩群體中均呈現(xiàn)低度多態(tài)(PIC0.25),多態(tài)水平較低。該多態(tài)位點基因型分布均符合Hardy-Weinberg平衡狀態(tài)(P0.05);Leptin基因Hinf I酶切位點在兩群體中均處于低度多態(tài)(PIC0.25),多態(tài)水平較低。該多態(tài)位點基因型分布在卵泡囊腫群體符合Hardy-Weinberg平衡狀態(tài)(P0.05),在健康豬群體背離Hardy-Weinberg平衡狀態(tài)(P0.05)。(3)利用分析軟件對ESR、FSHβ和Leptin的基因型分布進行分析,結(jié)果表明ESR的AA和AB基因型在健康群體和卵泡囊腫群體分布差異顯著(p0.05),與卵泡囊腫的形成有關(guān);FSHβ基因和leptin基因的基因型分布在本檢測群體中均未見顯著差異(p0.05),與卵泡囊腫的形成無關(guān)。ESR基因可作為卵泡囊腫篩選的標(biāo)志基因,為研發(fā)提高母豬繁殖力的新技術(shù)奠定基礎(chǔ)。
[Abstract]:Follicular cyst is one of the common reproductive disorders of sow, which can cause infertile sows. It can cause maternal animals to continue, frequent, transient and irregular. The disease has caused great obstacles to the development of pig breeding industry. At present, the more research of molecular biology is being made, the more research on the marker gene of porcine follicle cyst is found. The more emphasis is placed on.ESR, the FSH beta and Leptin genes can play an important role in the growth and development of the sow ovary by regulating each link of the hypothalamus pituitary ovary axis..ESR (estrogen receptor) is one of the members of the steroid receptor family. It can regulate the reproductive activities of female mammals and affect the sex gland tissue of female animals. The expression and regulation of these factors further affect the second sex, reproductive cycle, fertility, and pregnancy maintenance of the individual animal. The results show that the ESR gene Pvu II polymorphism of the pig gene has a significant influence on the litter size and reproductive traits of pigs. However, there is no report on the relationship between this site and follicle cyst,.FSH (follicle stimulation) at present. It is a glycoprotein type gonadotropin which plays an important role in promoting the maturation of granular cells. The FSH gene structure of the pig contains a reverse transposon of a length of 292bp, which leads to the polymorphism of the gene site due to the insertion of the transposon. After the analysis of this site, the FSH beta gene is the main effect on the reproductive traits of pigs. But there is no study of the FSH beta gene as a candidate gene to analyze its relationship with follicle cysts..Leptin (leptin) is a class of protein hormones secreted by adipocytes. Its gene expression products are mainly involved in the regulation of animal growth and reproductive function. Some studies have shown that the third exon T3469C of Porcine leptin gene is T3469C Loci variation has a certain effect on the reproductive performance of pigs. However, no study has been made to analyze the relationship between the Leptin gene as a candidate gene and the follicle cyst. This experiment was conducted by estrogen receptor (ESR), follicle stimulating hormone beta subunit (FSH beta) and leptin (Leptin). After collecting healthy and sick ovaries, the ovarian genes were extracted by extracting the ovarian genes. Group DNA, then use direct electrophoresis and PCR-RFLP method to detect the polymorphic loci of estrogen receptor (ESR), follicle stimulating hormone beta subunit (FSH beta) and leptin (Leptin), and further analyze the relationship between the loci and follicle cysts, and find the molecular markers related to follicle cysts. The main contents are as follows: (1) the results of gene polymorphism detection show: 3 A total of 4 polymorphic loci were detected in the ESR gene intron AG mutation, located in the TC mutation in the intron of the FSH beta gene and the CT mutation in the intron of the FSH beta gene, and the TC mutation in the exon of the leptin gene. (2) the polymorphism information content, heterozygosity and effective allele analysis showed that the ESR Pvu II polymorphic loci Moderate polymorphism (0.25PIC0.5) was present in the two population, which was distributed in the equilibrium state of Hardy-Weinberg in the cyst population (P0.05), and in the healthy pig population, which was in line with Hardy-Weinberg balance (P0.05), and the polymorphic loci of the FSH beta 5 'regulatory region showed low polymorphism (PIC0.25) in the follicular cysts, and all of the healthy pigs were all A moderate polymorphism (0.25PIC0.5). The polymorphism loci of the polymorphic loci were all deviated from the equilibrium state of Hardy-Weinberg (P0.05), and the FSH beta -Hae III polymorphic loci were low polymorphic in the two population (PIC0.25), and the polymorphism level was lower than that of Hardy-Weinberg equilibrium state (P0.05) and Leptin gene Hin. The f I loci were low polymorphism (PIC0.25) in the two population, and the polymorphic loci were distributed in the follicle cyst group with Hardy-Weinberg equilibrium state (P0.05) and Hardy-Weinberg balance in healthy pig population (P0.05). (3) the distribution of the genotype distribution of ESR, FSH beta and Leptin was divided by the use analysis software. The results showed that the distribution of AA and AB genotypes of ESR in healthy population and follicle cyst was significantly different (P0.05), which was related to the formation of follicle cysts. The genotype distribution of FSH beta gene and leptin gene was not significantly different in this test group (P0.05). It was not related to the formation of follicle swollen, which could be used as the marker of follicle cyst screening. Chi gene is the foundation for developing new technology to improve sow's fecundity.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：S828

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