本文選題：沙地云杉 + 內(nèi)生真菌��； 參考：《內(nèi)蒙古農(nóng)業(yè)大學(xué)》2017年碩士論文

【摘要】：本文以內(nèi)蒙古克什克騰旗白音敖包自然保護(hù)區(qū)的沙地云杉(Picea mongolica)為研究對(duì)象,通過對(duì)沙地云杉不同生態(tài)型(紅果型、紫果型、綠果型)、不同齡級(jí)(幼齡、中齡、老齡)和不同部位(枝部、針葉、球果、樹皮、根部)內(nèi)生真菌的分離、純化和鑒定,確定出優(yōu)勢(shì)菌。根據(jù)優(yōu)勢(shì)菌的生物學(xué)特性,判斷其適宜的生長環(huán)境。同時(shí),通過對(duì)優(yōu)勢(shì)菌的抑菌性研究,了解內(nèi)生真菌與病原菌之間的相互關(guān)系。結(jié)果表明:1.從沙地云杉中共分離出內(nèi)生真菌890株(其中未產(chǎn)孢37株),經(jīng)形態(tài)顯微觀察鑒定853株分屬于4目5科22屬,其中交鏈孢屬和青霉屬為優(yōu)勢(shì)菌。2.不同齡級(jí)的沙地云杉菌株分布不同,雖然菌株數(shù)在3個(gè)齡級(jí)之間相差不大,但是內(nèi)生真菌的種類隨著樹齡的增長在逐漸增加。沙地云杉不同部位的內(nèi)生真菌株數(shù)變化順序排列為枝部樹皮部球果針葉根部。3.紅果型沙地云杉和紫果型沙地云杉之間的內(nèi)生真菌種類相似性高。紅果型和綠果型沙地云杉之間相似性低。在多樣性分布上綠果型沙地云杉中分離得到的內(nèi)生真菌種群Shannon-Wiener多樣性指數(shù)和均勻度指數(shù)均為最高。紅果型沙地云杉獲得的內(nèi)生真菌種群Shannon-Wiener指數(shù)和均勻度指數(shù)均為最低。4.交鏈孢菌和青霉菌均在24h的光照下生長更為適宜;交鏈孢菌最適生長溫度為30℃,青霉菌的最適生長溫度為25℃;交鏈孢菌和青霉菌的最適的pH值分別為5.5和6;交鏈孢菌適宜的氮源和碳源分別是蛋白胨與麥芽糖,青霉菌適宜的氮源和碳源分別是酵母浸膏和蔗糖。5.交鏈孢菌對(duì)丁香褐斑病,楊樹褐斑病和楊樹爛皮病的抑菌效果較好;而青霉菌對(duì)沙棗褐斑病,柳樹黑斑病和楊樹灰斑病的抑菌效果好。
[Abstract]:In this paper, Picea mongolica (Picea mongolica) from Baiyinaobo Nature Reserve of Keshiketeng Banner in Inner Mongolia was studied. Different ecotypes (red fruit type, purple fruit type, green fruit type, different age class (young, middle age) of Picea mongolicia) were studied. Isolation, purification and identification of endophytic fungi from different parts (branches, needles, cones, bark and roots), and identification of dominant fungi. According to the biological characteristics of dominant bacteria, the suitable growth environment was judged. At the same time, the relationship between endophytic fungi and pathogens was studied by studying the bacteriostasis of dominant bacteria. The results show that: 1. A total of 890 strains of endophytic fungi were isolated from Picea spruce. Among them, 37 strains did not produce spore. By morphological and microscopic observation, 853 strains were identified as belonging to 22 genera, 5 families and 4 orders, among which Alternaria and Penicillium were the dominant bacteria. The distribution of spruce strains in different age classes was different. Although the number of spruce strains was not different among the three age classes, the species of endophytic fungi increased gradually with the increase of tree age. The number of endophytic fungi in different parts of Picea spruce was arranged in the order of branch bark cone coniferous root. The similarity of endophytic fungi between red fruit spruce and purple fruit spruce is high. The similarity between red fruit type and green fruit type spruce is low. The Shannon-Wiener diversity index and evenness index of endophytic fungi isolated from spruce were the highest in diversity distribution. The Shannon-Wiener index and evenness index of endophytic fungi obtained from Picea rubra were the lowest. 4. The optimum growth temperature was 30 鈩，

本文編號(hào)：1971538