本文選題：忍冬 + 鹽脅迫�。� 參考：《山東中醫(yī)藥大學(xué)》2015年碩士論文

【摘要】：目的:分析鹽脅迫對(duì)紅金銀花和忍冬光合參數(shù)包括凈光合速率、氣孔導(dǎo)度、胞間二氧化碳濃度、蒸騰速率及金銀花品質(zhì)的影響,進(jìn)而探討影響忍冬光合作用的決定性脅迫因子,為鹽漬土壤引種忍冬提供一定的理論依據(jù)。方法:采用NaCl溶液、中性鹽(NaCl:Na2SO4)、堿性鹽(NaHCO3:Na2CO3)、混合鹽(NaCl、Na2SO4、NaHCO3、Na2CO3)處理紅金銀花及忍冬扦插苗。用光合儀測(cè)定光合參數(shù),采用方差分析、相關(guān)性分析及逐步回歸分析評(píng)價(jià)鹽脅迫對(duì)紅金銀花和忍冬光合作用的影響,采用高效液相色譜法測(cè)定金銀花中馬錢(qián)苷、蘆丁、新綠原酸、綠原酸、隱綠原酸、木犀草苷、咖啡酸、異綠原酸B、異綠原酸A和異綠原酸C等10種有效成分含量。結(jié)果:①0.1%和0.3%NaCl溶液處理后的紅金銀花凈光合速率均比對(duì)照組高,而0.3%NaCl溶液處理下忍冬的凈光合速率在處理第四天比對(duì)照組低;0.5%NaCl脅迫處理后的金銀花中有效成分含量最高。②中性鹽脅迫、堿性鹽脅迫下,忍冬葉片凈光合速率隨鹽濃度的增加均呈先上升后下降的趨勢(shì);在中性鹽濃度為15 mmol·L-1,堿性鹽濃度為5 mmol·L-1時(shí),忍冬植株光合作用最強(qiáng),金銀花中黃酮類(lèi)成分的含量隨著中性鹽脅迫濃度的增加先升高后下降;中性鹽和堿性鹽脅迫均不利于黃酮類(lèi)成分的合成;在12.5、25、50 mmol·L-1中性鹽脅迫下,環(huán)烯醚萜苷成分的含量明顯高于對(duì)照組,其余各濃度處理組的環(huán)烯醚萜苷成分含量均低于對(duì)照組;酚酸類(lèi)成分的含量隨著中性鹽濃度的增加先下降后上升,而在堿性鹽脅迫下隨著鹽濃度的增加先上升后下降,且各處理組的含量均比對(duì)照組低。相關(guān)性分析及回歸分析結(jié)果表明,鹽度、緩沖量、pH為三個(gè)主要脅迫因子,且鹽度是抑制忍冬植株光合作用的主導(dǎo)因素,隨著脅迫時(shí)間的延長(zhǎng),pH成為影響植株光合作用的次要因素。結(jié)論:NaCl脅迫條件下,紅金銀花的抗鹽性高于忍冬,NaCl濃度為0.5%時(shí)金銀花中有效成分的含量最高;中性鹽脅迫和堿性鹽脅迫均不利于金銀花中黃酮類(lèi)成分的積累,適宜的鹽濃度有利于環(huán)烯醚萜苷成分的積累,高濃度中性鹽脅迫有利于酚酸類(lèi)成分的積累,堿性鹽脅迫不利于酚酸類(lèi)成分的積累;混合鹽脅迫下,鹽度為影響忍冬植株光合作用的決定性因素。
[Abstract]:Objective: to analyze the effects of salt stress on the photosynthetic parameters of Honeysuckle and Lonicera japonica, including net photosynthetic rate, stomatal conductance, intercellular carbon dioxide concentration, transpiration rate and quality of honeysuckle. It provides some theoretical basis for the introduction of Lonicera japonica in saline soil. Methods: Honeysuckle and Lonicera japonica cuttings were treated with NaCl solution, NaClO _ 3: Na _ 2SO _ 4, NaHCO _ 3: Na _ 2CO _ 3 and NaHCO _ 3H _ 2SO _ 4 + NaHCO _ 3H _ 2CO _ 3. Photosynthetic parameters were measured by photosynthetic apparatus, and the effects of salt stress on photosynthesis of Honeysuckle and Lonicera japonica were evaluated by variance analysis, correlation analysis and stepwise regression analysis. The contents of 10 active components, such as neo- acid, Lv Yuan acid, crypto- acid, luteolin, caffeic acid, isobali_person2# acid B, isob_person2# acid A and iso acid C, etc. Results the net photosynthetic rate of Honeysuckle treated with 10. 1% and 10. 1% 0.3%NaCl solution was higher than that of the control group. However, the net photosynthetic rate of Lonicera japonica in 0.3%NaCl solution was lower than that in control group on the fourth day. The content of active components in honeysuckle treated with 0.3%NaCl solution was the highest under neutral salt stress and alkaline salt stress. The net photosynthetic rate of Lonicera japonica leaves increased first and then decreased with the increase of salt concentration, and the photosynthesis of Lonicera japonica was the strongest when the neutral salt concentration was 15 mmol L -1 and the alkaline salt concentration was 5 mmol L -1. The content of flavonoids in Flos Lonicerae increased first and then decreased with the increase of neutral salt stress concentration, and both neutral salt and alkaline salt stress were not conducive to the synthesis of flavonoids, and under the neutral salt stress of 12.5 ~ 2550 mmol 路L ~ (-1), the content of flavonoids in Flos Lonicerae increased and then decreased with the increase of concentration of neutral salt. The contents of iridoid glycosides were significantly higher than those of the control group, the contents of iridoid glycosides in the other groups were lower than those in the control group, and the contents of phenolic acids decreased first and then increased with the increase of neutral salt concentration. Under alkaline salt stress, the concentration of salt increased first and then decreased, and the content of each treatment group was lower than that of the control group. The results of correlation analysis and regression analysis showed that salinity, buffer amount and pH were the three main stress factors, and salinity was the dominant factor to inhibit photosynthesis of Lonicera japonica. With the extension of stress time, pH was the secondary factor affecting plant photosynthesis. Conclusion the salt resistance of Honeysuckle is higher than that of Lonicera japonicus when the concentration of NaCl is 0.5, while neutral salt stress and alkaline salt stress are not conducive to the accumulation of flavonoids in honeysuckle. The suitable salt concentration is favorable to the accumulation of iridoid glycosides, the high concentration of neutral salt is favorable to the accumulation of phenolic acids, and the alkaline salt stress is not conducive to the accumulation of phenolic acids. Salinity is the decisive factor affecting photosynthesis of Lonicera japonica.
【學(xué)位授予單位】：山東中醫(yī)藥大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類(lèi)號(hào)】：S567.79

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