本文選題：香蘑屬 + 種群�。� 參考：《沈陽(yáng)農(nóng)業(yè)大學(xué)》2017年碩士論文

【摘要】：紫丁香蘑色澤宜人、菇香獨(dú)特,在我國(guó)主要分布在北方地區(qū)。紫丁香蘑作為東北地區(qū)珍稀的野生食藥用菌資源,由于其營(yíng)養(yǎng)價(jià)值和藥用價(jià)值不斷被人們發(fā)現(xiàn)和認(rèn)可,導(dǎo)致市場(chǎng)需求量不斷增加,野生資源有限且人工栽培過(guò)程比較困難,這對(duì)紫丁香蘑野生資源的保護(hù)是極其不利的。利用分子標(biāo)記的方法研究我國(guó)東北地區(qū)紫丁香蘑的遺傳多樣性,有助于了解它們的生存現(xiàn)狀,在資源保護(hù)和栽培育種等方面都具有重要的意義。通過(guò)SRAP和ISSR分子標(biāo)記方法對(duì)我國(guó)東北地區(qū)8個(gè)野生紫丁香蘑種群的72份樣本材料進(jìn)行研究分析。在SRAP分析中,從8個(gè)前端引物和8個(gè)后端引物組合成的64個(gè)引物組合中篩選出條帶多態(tài)性好、重現(xiàn)性和清晰度高的6個(gè)SRAP引物組合,擴(kuò)增紫丁香蘑的開放閱讀框序列區(qū)域,總共獲得117個(gè)條帶,其中多態(tài)性條帶有111條,多態(tài)性條帶百分比為94.87%。在紫丁香蘑的物種水平上,Nei's基因多樣性指數(shù)He = 0.3426,Shannon's遺傳多樣性信息指數(shù)I = 0.5049;種群間的Nei's平均遺傳距離為0.2059,遺傳分化系數(shù)Gst = 0.2136,基因流Nm= 1.8408,Nei's遺傳距離和種群地理距離之間曼特爾相關(guān)性檢驗(yàn)結(jié)果為r = 0.3377,P= 0.0580。在ISSR分析中,從23條ISSR引物中篩選出條帶多態(tài)性好、重現(xiàn)性和清晰度高的6條ISSR引物擴(kuò)增紫丁香蘑的重復(fù)序列,總共獲得97個(gè)條帶,其中多態(tài)性條帶有91條,多態(tài)性條帶百分比為93.81%。在紫丁香蘑的物種水平上,Nei's基因多樣性指數(shù)He = 0.3393,Shannon's遺傳多樣性信息指數(shù)I=0.5033;種群間的Nei's平均遺傳距離為0.2557,遺傳分化系數(shù)Gst = 0.2666,基因流Nm = 1.3752,種群間的Nei's遺傳距離和地理距離之間曼特爾相關(guān)性檢驗(yàn)結(jié)果為r =0.1850,P = 0.1908。實(shí)驗(yàn)結(jié)果表明兩種分子標(biāo)記方法均適合用于分析紫丁香蘑的遺傳多樣性,都檢測(cè)到紫丁香蘑具有較高的遺傳多樣性,其中SRAP標(biāo)記多態(tài)性略高于ISSR標(biāo)記。本研究結(jié)果表明紫丁香蘑不同種群間存在一定的遺傳分化和基因流動(dòng),遺傳分化主要發(fā)生在種群內(nèi),遺傳距離和地理距離之間沒(méi)有明顯的相關(guān)性關(guān)系。
[Abstract]:Lilac mushroom color is pleasant, mushroom fragrance unique, mainly distributed in the north of China.As a rare resource of wild edible medicinal fungi in Northeast China, lilac mushroom has been found and recognized by people because of its nutritional value and medicinal value, which leads to increasing market demand, limited wild resources and difficult artificial cultivation process.This is extremely disadvantageous to the conservation of wild resources of lilac mushroom.Using molecular markers to study the genetic diversity of Tricholoma lilacs in Northeast China is helpful to understand their survival status and is of great significance in conservation of resources and cultivation and breeding.SRAP and ISSR molecular markers were used to study 72 samples of 8 wild populations of Tricholoma lilacs in Northeast China.In SRAP analysis, from 64 primer combinations composed of 8 front-end primer and 8 back-end primer, 6 SRAP primer combinations with high reproducibility and clarity were selected to amplify the open reading frame region of Tricholoma lanceolata.A total of 117 bands were obtained, of which 111 were polymorphic, the percentage of polymorphic bands was 94.87.The genetic diversity index he = 0.3426 Shannons genetic diversity information index I = 0.5049, the average Nei's genetic distance between populations is 0.2059, the genetic differentiation coefficient Gst = 0.2136, the gene flow Nm = 1.8408Neis genetic distance and population geography.The result of Mantel correlation test between distances was r = 0.3377 (P = 0.0580).In ISSR analysis, 6 ISSR primers with high reproducibility and clarity were selected from 23 ISSR primers to amplify the repeat sequence of Tricholoma lilygii. A total of 97 bands were obtained, among which 91 were polymorphic.The percentage of polymorphic bands was 93.81%.At the species level of Tricholoma lilygii, the genetic diversity index of Neis is he = 0.3393 and Shannons genetic diversity information index is 0.5033, the average genetic distance of Nei's among populations is 0.2557, the coefficient of genetic differentiation Gst = 0.2666, the gene flow Nm = 1.3752, the Nei's genetic distance between populations and the genetic distance between populations.The result of Mantel correlation test between geographical distance is r = 0.1850 P = 0.1908.The results showed that both of the two molecular markers were suitable for the analysis of genetic diversity of Tricholoma lilac, and both of them had higher genetic diversity, and the polymorphism of SRAP marker was slightly higher than that of ISSR marker.The results showed that there was a certain genetic differentiation and gene flow among different populations of Tricholoma lilacs. Genetic differentiation mainly occurred within the population, and there was no obvious correlation between genetic distance and geographical distance.
【學(xué)位授予單位】：沈陽(yáng)農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：S646
，

本文編號(hào)：1773775