本文選題：大豆 + 生育期　； 參考：《聊城大學》2017年碩士論文

【摘要】：生育期和分枝數(shù)都是影響大豆栽培措施和產量的重要農藝性狀,對影響這兩個性狀的QTL座位進行定位具有重要的理論和現(xiàn)實意義。為定位生育期相關QTL座位,本研究以栽培大豆菏豆14為母本,野生大豆ZYD02810為父本,構建生育期分離群體。在F_2代,利用選擇基因型方法進行生育期初步QTL座位定位,結果表明C2連鎖群上Satt307(121.27 cM)和C1連鎖群上Satt718(73.79cM)座位與生育期相關。進一步利用F_2群體中篩選出的F_(2:3)晚熟殘余雜合株系繼續(xù)種植分離群體對上述定位結果進行驗證。結果表明,對于Satt307座位,無論哪種方法,均能得到驗證;而對于Satt718座位只在F_2單尾中得到驗證,F_(2:3)晚熟殘余雜合株系沒有得到驗證,其結論尚需進一步驗證。另外,通過對已克隆成熟期基因E1~E4測序結果表明,這些基因在親本間沒有差異,表明本研究中的生育期性狀與這些基因沒有關系。為定位大豆分枝數(shù)相關QTL,本研究以寡分枝栽培大豆冀黃13為母本,多分枝地方品種小黑豆為父本配制雜交組合,在2012年徐艷萍利用F_(2:3)群體通過寡分枝單尾法選擇性基因分型方法定位分枝數(shù)QTL的基礎上,在2014,我們又利用F_2:4群體通過雙尾法選擇性基因分型方法對大豆分枝數(shù)進行QTL定位。研究表明2014年,在F_2:4分離群體中的雙尾群體中共檢測到2個與分枝數(shù)QTL座位連鎖的座位,分別是F連鎖群上的BARC13-1845座位和B2連鎖群上的BARC14-1214座位;而在2012年利用寡分枝單尾群體檢測到的L連鎖群上BARC19-1222(71.32cM)座位,在2014年并沒有被檢測到。在2014年檢測到的兩個分枝數(shù)QTL座位附近,沒有其他分枝數(shù)相關QTL座位的報道,這兩個座位可能是新的。本研究將為進一步進行分枝數(shù)QTL座位的精細定位和分子標記輔助選擇育種奠定基礎。
[Abstract]:The growth period and branching number are important agronomic characters that affect soybean cultivation measures and yield. It is of great theoretical and practical significance to map the QTL loci which affect the two traits.In order to localize the QTL loci related to growth period, a segregated population was constructed by using cultivated soybean Headou 14 as female parent and wild soybean ZYD02810 as male parent.The preliminary QTL loci were mapped by genotypic selection in the second generation of FSCL. The results showed that Satt307(121.27 cM) on C2 linkage group and Satt7181873.79cM) locus on C1 linkage group were correlated with growth period.The results were further verified by using the late mature residual heterozygous lines, which were selected from the F _ (2) population, and continued to be planted and segregated.The results show that the Satt307 locus can be verified by either method, but the Satt718 locus can only be verified in the single tail of FSC2. The results show that the residual heterozygous lines of late maturing can not be verified, and the conclusion needs further verification.In addition, E1~E4 sequencing of cloned mature genes showed that there was no difference between parents, indicating that the growth stage traits in this study were not related to these genes.In order to locate the QTL-based branch number correlation of soybean, the hybrid combination was prepared by using oligobranched soybean Jihuang 13 as female parent and multi-branched local variety Xiaotou bean as male parent.In 2012, Xu Yanping used Fsta2: 3) to locate the number of branches (QTL) by the method of selective genotyping with oligobranching single tail method. In 2014, we used F2: 4 population to localize the number of branches of soybean by using the method of two-tail selective genotyping.The results showed that in 2014, two loci linked to branched QTL loci were detected in the two-tailed population of FSCL, namely BARC13-1845 locus in F linkage group and BARC14-1214 locus in B2 linkage group.However, no BARC19-122222 71.32cM locus was detected in the L linkage group detected in 2012 using oligobranched single-tailed populations, but was not detected in 2014.There were no reports of other branched QTL loci near the two branched QTL loci detected in 2014, which may be new.This study will lay a foundation for further fine mapping of branch number QTL loci and molecular marker-assisted selection breeding.
【學位授予單位】：聊城大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：S565.1

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