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  本文選題：有機(jī)磷農(nóng)藥　切入點：分子印跡聚合物　出處：《山東農(nóng)業(yè)大學(xué)》2017年碩士論文

【摘要】：有機(jī)磷農(nóng)藥廣泛應(yīng)用于作物的病蟲害防治以提高作物的產(chǎn)量和質(zhì)量,但是其大量使用會使環(huán)境遭受污染,并危及人類的健康。因此建立有機(jī)磷農(nóng)藥殘留的檢測方法具有重要的意義。本實驗基于分子印跡技術(shù)及魯米諾-過氧化氫化學(xué)發(fā)光體系,建立了兩種有機(jī)磷農(nóng)藥殘留的檢測方法,主要研究結(jié)果如下:1.采用本體聚合法,以久效磷為模板分子,甲基丙烯酸為功能單體,乙二醇二甲基丙烯酸酯為交聯(lián)劑,偶氮二異丁腈為引發(fā)劑,合成了久效磷分子印跡聚合物。靜態(tài)吸附、動態(tài)吸附及選擇性實驗表明所制備的印跡聚合物對久效磷具有較好的選擇性和特異吸附性。基于久效磷在堿性介質(zhì)中能夠增強(qiáng)魯米諾-過氧化氫化學(xué)發(fā)光反應(yīng),以制備的分子印跡聚合物作為吸附劑,建立了一種久效磷的分子印跡固相萃取-流動注射化學(xué)發(fā)光分析方法。結(jié)果顯示在最優(yōu)條件下,化學(xué)發(fā)光增強(qiáng)值與久效磷濃度在0.0023~10mg/L呈良好的線性關(guān)系,最低檢出限為0.001 mg/L。對油菜和小白菜分別在三個水平上進(jìn)行了添加回收實驗,兩種樣品的回收率為89.3%~105.9%。2.以合成的敵百蟲-BSA-魯米諾作為化學(xué)發(fā)光標(biāo)記物,本體聚合法合成的敵百蟲分子印跡聚合物作為仿生抗體,建立了敵百蟲流動注射化學(xué)發(fā)光仿生免疫分析方法,并對影響競爭性免疫反應(yīng)的條件進(jìn)行了優(yōu)化。結(jié)果表明以pH 7.2的PBS作為樣品稀釋液,過氧化氫濃度為0.4 mol/L,該方法的靈敏度(IC50)為2 mg/L,最低檢出限(LOD,IC15)為0.0024 mg/L,本方法對胡蘿卜和卷心菜的添加回收率在76.7~89.7%之間。
[Abstract]:Organophosphorus pesticides are widely used in crop pest and disease control to improve crop yield and quality, but their extensive use can cause environmental pollution. Therefore, it is of great significance to establish a method for the detection of organophosphorus pesticide residues. This study was based on molecular imprinting and luminol hydrogen peroxide chemiluminescence system. Two methods for the determination of organophosphorus pesticide residues were established. The main results were as follows: 1. Monocrotophos was used as template molecule, methacrylic acid as functional monomer and ethylene glycol dimethacrylate as crosslinking agent. Monocrotophos molecularly imprinted polymer was synthesized by using azodiisobutyronitrile as initiator. The dynamic adsorption and selective experiments showed that the imprinted polymer had good selectivity and specific adsorbability for monocrotophos, and the monocrotophos could enhance the chemiluminescence reaction of luminol and hydrogen peroxide in alkaline medium. Using the molecularly imprinted polymer as adsorbent, a monocrotophos method for solid-phase extraction and flow injection chemiluminescence analysis of monocrotophos was established. There was a good linear relationship between chemiluminescence enhancement value and monocrotophos concentration in 0.0023~10mg/L. The lowest detection limit was 0.001 mg / L. The experiments of adding and recovering rape and pakchoi at three levels were carried out. The recoveries of the two samples were 89.3% and 105.9%, respectively. Using the synthesized trichlorfon -BSA-Lumino as chemiluminescence marker, the molecularly imprinted polymer of trichlorfon was synthesized by bulk polymerization as bionic antibody. A flow injection chemiluminescent bionic immunoassay for trichlorfon was established, and the conditions affecting the competitive immune reaction were optimized. The results showed that pH 7.2 PBS was used as the sample dilution. The concentration of hydrogen peroxide was 0.4 mol / L, the sensitivity of the method was 2 mg / L and the lowest detection limit was 0.0024 mg / L. The recovery rate of the method for carrot and cabbage was 76.7% and 89.7% respectively.
【學(xué)位授予單位】：山東農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：S481.8

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 彭麗芳;李樺;;縮膽囊素神經(jīng)肽分子印跡膜的制備及熒光免疫分析應(yīng)用[J];分析科學(xué)學(xué)報;2017年01期

2 劉蒙;劉s，

本文編號：1659955