本文選題：王不留行　切入點：黃酮苷　出處：《西南大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：本課題旨在探究向泌乳期母兔日糧中加入不同劑量的王不留行提取物對母兔泌乳及哺乳性能的影響,為王不留行黃酮苷在實際生產(chǎn)中的應(yīng)用提供相應(yīng)的理論基礎(chǔ)。試驗選擇150只、體況相近、仔數(shù)一致的健康新西蘭經(jīng)產(chǎn)哺乳母兔(每胎產(chǎn)活仔數(shù)為10-12只),分三個周期,每個周期選擇50只母乳隨機分為5組(對照組和4個試驗組),每個組10只母兔,試驗組分別按1、2、4和8g/d的劑量在日糧中加入含有王不留行提取物(分別含王不留行黃酮苷0.004、0.008、0.016和0.032g/d),從分娩第1天開始添加,直至28日斷奶,空白組的日糧和飼喂條件與試驗組相同。在哺乳期間觀測并記錄下仔兔體重的變化、仔兔腹瀉率和斷奶存活率的情況、母兔血清中部分相關(guān)激素的變化、母乳中乳糖和乳鐵蛋白的變化,在第28天斷奶時隨機抽取母兔解剖采集乳腺樣本對相關(guān)基因的表達量和RNA/DNA比值進行測定,制作乳腺組織石蠟切片觀測各組腺泡情況。試驗結(jié)果:(1)日糧中添加王不留行提取物對斷奶時仔兔生長性能的影響。母兔日糧中王不留行黃酮苷的添加對仔兔斷奶時的體重有顯著的影響,其中4g組和8g組顯著高于空白組和1g組(P0.05);仔兔哺乳期的腹瀉率受到顯著影響,2g組、4g組和8g組仔兔的腹瀉率低于空白組;4g組和8g組仔兔的斷奶存活率高于空白組。(2)日糧中添加王不留行提取物對哺乳期母兔血清中部分激素和母乳質(zhì)量的影響。日糧中添加王不留行黃酮苷對母兔血清中雌激素的含量無顯著影響(P0.05);王不留行黃酮苷的添加可減緩母兔血清中PRL的含量下降趨勢,極顯著的增加PRL的含量,各試驗組PRL的含量均顯著或極顯著高于空白組(P0.05,P0.01);GH含量下降的趨勢可得到減緩,極顯著的增加GH的含量,各試驗組GH的含量均顯著或極顯著高于空白組(P0.05,P0.01)。日糧中添加王不留行黃酮苷可使母兔母乳中乳鐵蛋白的含量在泌乳前期(第5至第9天)顯著或極顯著高于空白組(P0.05,P0.01),并在泌乳末期保持顯著差異(P0.05);乳中乳糖的含量也會受到顯著變化,泌乳的中后期(第13至第25天)試驗組的乳糖含量可極顯著高于空白組(P0.01),直至斷奶時試驗組的乳糖含量仍可顯著高于空白組(P0.05)。(3)日糧中添加王不留行提取物對母兔乳腺的影響。泌乳期母兔日糧中添加一定量的王不留行黃酮苷對LACE-1、IGF-1和EGF的基因表達量有不同程度的影響。王不留行黃酮苷的添加可以顯著提高LACE-1和IGF-1基因的表達量(P0.05),可極顯著的影響EGF基因的表達量(P0.01)。王不留行黃酮苷的添加對乳腺組織中RNA/DNA比值有顯著的降低(P0.05)。從乳腺組織的切片中可以看出王不留行黃酮苷的添加可以增加乳腺組織在斷奶時仍具有泌乳活性的腺泡的數(shù)量。綜上所述,向泌乳期母兔的日糧中添加王不留行提取物可以顯著提高仔兔斷奶時的體重,降低腹瀉率,增加斷奶存活率,并能提高母兔血清中PRL和GH的含量,提高母乳中乳鐵蛋白和乳糖的含量,增加對LACE-1、IGF-1和EGF的基因表達量,降低RNA/DNA比值,增加乳腺中具有泌乳活性的腺泡的數(shù)量。實驗結(jié)果表明,在添加量為4 g/d王不留行提取物(含王不留行黃酮苷0.016 g/d)時具有最佳的生產(chǎn)性能。
[Abstract]:The purpose of this study is to explore the influence of cowherb seed extracts with different doses to lactation mother rabbit diets on lactation and lactation performance of female rabbit king, without leaving the line in the application of flavonoid glycosides in actual production to provide the corresponding theoretical foundation. Experiment 150, similar body condition, litter size consistent multiparous lactation female healthy New Zealand rabbit (live births per litter was 10-12), divided into three cycles, each cycle of 50 breast milk were randomly divided into 5 groups (control group and 4 experimental groups), each group of 10 female rabbits, experimental groups were 1,2,4 and 8g/d dose in the diets added with cowherb seed extracts (with vaccarin glycosides 0.004,0.008,0.016 and 0.032g/d), from first days to start adding delivery, until 28 days weaning, blank group diet and feeding conditions and test of the same group. Observe and record the changes of rabbit under weight during lactation, and diarrhea rate of newborn rabbits Weaning survival rate, changes in hormone related female rabbit serum, the change of lactose and lactoferrin in breast milk, on the twenty-eighth day weaning female rabbits randomly selected samples of breast anatomy acquisition related gene expression and RNA/DNA ratio were measured, making breast tissue paraffin sections were observed. Results: (acinar 1) add cowherb seed extracts in diets on growth performance of weaned pups affected. Add female rabbit diets vaccarin glycosides have significant effect on weaning weight at which, 4G group and 8g group was significantly higher than the control group and 1g group (P0.05); rabbit lactation diarrhea rate significantly affected, 2G group, 4G group and 8g group rabbit diarrhea rate lower than that of the control group; 4G group and 8g group of young rabbits weaning survival rate higher than the control group. (2) add cowherb seed extracts in diet on serum induced part of female rabbit lactation And the quality of breast milk. The effect has no significant effect on content of flavonoid glycosides added in diet on the mother of estrogen in rabbit serum (P0.05); the flavone glycosides can slow add mother rabbit serum PRL content decreased significantly, the content of PRL increased, the content of PRL of the experimental group were significant or extremely significantly higher than the control group (P0.05, P0.01); the content of GH decreased can be slowed, significantly increase the contents of GH, GH content in each experimental group were significantly higher than the control group (P0.05, P0.01). The added dietary flavonoid glycosides can make the content of lactoferrin in human milk in female rabbits the lactation period (fifth to ninth days) was significantly higher than the control group (P0.05, P0.01), and significant difference in late lactation (P0.05); lactose content in milk will also be significantly changed in the late lactation (thirteenth to twenty-fifth days) the test group of lactose The content can be significantly higher than the control group (P0.01), until weaning the lactose content in experimental group is still significantly higher than the control group (P0.05). (3) effects of Vaccaria segetalis extract in diet on rabbit breast. Vaccarin glycosides by adding a certain amount of lactation mother rabbit diet on LACE-1, IGF-1 and EGF the amount of gene expression have different effects. Vaccarin glycosides added increased expression of LACE-1 and IGF-1 gene (P0.05), can influence the expression of EGF gene significantly (P0.01). The flavone glycosides of RNA/DNA added milk gland tissue ratio decreased significantly (P0.05). Breast tissue slices can be seen in the number of add Vaccaria flavone glycosides can increase the breast tissue is still active at weaning milk gland. In summary, Wang added without leaving the line to extract diet during lactation in female rabbits In order to improve the weaning weight, reduce the diarrhea rate, increase the survival rate of weaning, and can increase the content of PRL and GH in serum of female rabbits, increase the content of lactoferrin and lactose in breast milk, increase of LACE-1, expression of IGF-1 and EGF gene, reduce the ratio of RNA/DNA, the number has increased the activity of breast milk in the acini. The experimental results show that the addition of 4 g/d (including vaccarin cowherb seed extracts by 0.016 g/d) has the best manufacturing performance.

【學(xué)位授予單位】：西南大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：S829.1

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