本文選題：茶呰子葉狀層菌　切入點(diǎn)：粉托鬼筆　出處：《魯東大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：茶呰子葉狀層菌、粉托鬼筆、短裙竹蓀均是野生食藥用真菌。據(jù)研究,茶呰子葉狀層菌與金銀花的功效有相似之處,可用于消炎、解毒、止痛等,目前已被制成保健品制劑,對各類炎癥及感冒有極好的療效。粉托鬼筆首先由本實(shí)驗(yàn)室進(jìn)行研究,在本實(shí)驗(yàn)室中已栽培出子實(shí)體,但尚未進(jìn)行大規(guī)模的栽培。短裙竹蓀是一種味道極佳的食藥用真菌,具有降血脂,調(diào)節(jié)脂肪酸預(yù)防高血壓的作用。目前,這三種野生食藥用真菌采摘量有限,尚未進(jìn)行過系統(tǒng)的菌絲培養(yǎng)研究,也限制了它們的藥理研究。本論文進(jìn)行了茶呰子葉狀層菌、粉托鬼筆的菌種分離及鑒定、固體培養(yǎng)特性和液體發(fā)酵培養(yǎng)條件及菌絲化學(xué)成分含量研究。短裙竹蓀的固體培養(yǎng)和發(fā)酵罐發(fā)酵培養(yǎng)條件及菌絲化學(xué)成分含量研究。為以后進(jìn)行大規(guī)模的工業(yè)發(fā)酵生產(chǎn)做好準(zhǔn)備工作。首先對采集的野生茶呰子葉狀層菌子實(shí)體進(jìn)行菌種分離及菌種鑒定,確定組織分離所得菌絲體即為茶呰子葉狀層菌菌絲體。采用固體皿培法研究固體培養(yǎng)特性:發(fā)現(xiàn)茶呰子葉狀層菌菌絲體在麥麩、麥芽汁、胡蘿卜、PDA、GPC、GPY固體培養(yǎng)基上均能生長,在麥芽汁固體培養(yǎng)基上生長速度最快,在第50天時(shí)的麥麩固體培養(yǎng)基上生物量最大,在第40天時(shí)GPY培養(yǎng)基上的單位質(zhì)量的菌絲體中三萜含量為0.39%,考慮獲得三萜的總量最高,應(yīng)選擇60天的麥麩培養(yǎng)基;茶呰子葉狀層菌對碳源和有機(jī)氮源的適應(yīng)性較強(qiáng),在麥芽糖、果糖、木糖、乳糖上生長較好,除了在尿素中不能生長,在其他氮源中均能生長。采用搖瓶發(fā)酵法篩選發(fā)酵培養(yǎng)基:茶呰子葉狀層菌在只有碳源+氮源+生長因子的培養(yǎng)液中也能生長,但在麥芽汁培養(yǎng)液中生長最好,麥芽汁培養(yǎng)液適合作為茶呰子葉狀層菌的種子培養(yǎng)液和發(fā)酵培養(yǎng)液;在搖瓶發(fā)酵中,三萜含量不低于1%,多糖含量在15%以上,第7天的生物量達(dá)到最大,同時(shí)采用HPLC測得腺苷獲得量最多。隨著液體發(fā)酵時(shí)間的延長,茶呰子葉狀層菌菌球顏色逐漸由乳白色變?yōu)樽睾稚?且發(fā)酵液顏色也逐漸變?yōu)樽睾稚?并有一股清新的果香味。本研究采用組織分離法對粉托鬼筆菌種進(jìn)行菌種分離并進(jìn)行分子生物學(xué)鑒定得出分離所得菌絲體為粉托鬼筆。粉托鬼筆在不同固體培養(yǎng)基上均能生長,在麥麩、麥芽汁、胡蘿卜、PDA固體培養(yǎng)基上生長較好,粉托鬼筆對碳源和氮源的適應(yīng)性較好,在不同碳源、氮源條件下,其菌絲的生長速度和氣生菌絲的形態(tài)有較大差異。通過不同培養(yǎng)液及不同碳源及氮源對液體發(fā)酵培養(yǎng)基的篩選發(fā)現(xiàn):PDA培養(yǎng)液適合作為粉托鬼筆的種子培養(yǎng)液,在10L的發(fā)酵罐中投料7L,蔗糖140g,大豆蛋白胨42g,酵母浸粉70g,接種量5%,溫度26℃,轉(zhuǎn)速150rpm,通氣量1:0.3,培養(yǎng)至58h適合放罐。菌絲體中多糖含量達(dá)18.02%。適合作為判斷放罐標(biāo)準(zhǔn)的指標(biāo)是菌絲生物量及發(fā)酵液總糖含量。短裙竹蓀菌絲體在所試不同培養(yǎng)上均能生長,在GPY、PDA、麥芽汁、麥麩、胡蘿卜固體培養(yǎng)基上生長較好,在GPY固體培養(yǎng)基上生長速度最快,生物量最大,為145.50mg。短裙竹蓀對碳源和有機(jī)氮源的適應(yīng)性很強(qiáng)。短裙竹蓀液體種子培養(yǎng)基為PDA培養(yǎng)液,在10L的發(fā)酵罐中投料7L,蔗糖140g,大豆蛋白胨42g,酵母浸粉70g,接種量5%,溫度26℃,轉(zhuǎn)速150rpm,通氣量1:0.2,培養(yǎng)至90h適合放罐。菌絲體中多糖含量達(dá)17.92%。
[Abstract]:Tea Zi Zi leaflike layer fungus, phallus hadriani, Dictyophora duplicata is wild edible and medicinal fungi. According to the study, tea Zi sub leaflike layer fungus and honeysuckle efficacy is similar, can be used for detoxification, anti-inflammatory, analgesic, has now been made health care preparation, has an excellent efficacy of inflammation and cold. Phallus hadriani first by this laboratory study in our laboratory has cultivated a fruiting body, but not for large-scale cultivation. Dictyophora duplicata edible fungus is an excellent taste, with lowering blood pressure, regulating fatty acid to prevent hypertension. At present, these three kinds of wild edible and medicinal fungi picking limited that has not yet been studied culture system of mycelium, also limits the pharmacological research on them. This paper tea Zi sub leaflike layer fungus, bacteria isolation and identification of phallus hadriani, solid culture and liquid fermentation conditions of mycelium and chemical Research contents. Solid culture and culture of Dictyophora duplicata conditions and chemical composition content of mycelium fermentation. For industrial fermentation production of large-scale preparations. Firstly, wild tea Zi sub leaflike layers sporophore collected bacteria isolation and identification, determine the tissue isolated from mycelium is tea Zi Zi the leaflike layer fungus mycelium. The solid plate culture method on solid culture characteristics: Tea Zi sub leaflike layer fungus mycelium in wheat bran, malt juice, carrot, PDA, GPC, GPY solid medium growth can in wort solid medium, the fastest growth rate in the fiftieth day wheat bran solid medium maximum biomass, on the fortieth day GPY three mycelium cultured on terpene content of unit mass of the body is 0.39%, the highest total consideration for three terpene, should choose 60 days of wheat bran medium; tea Zi Zi The leaflike layer fungus with carbon and organic nitrogen in adaptability, maltose, fructose, xylose, galactose on growth is better, in addition to not grow in urea, can grow in other nitrogen sources were screened. The fermentation medium by shaking flask fermentation method: Tea Zi sub leaflike layer fungus can grow in culture only the liquid carbon source nitrogen source + + growth factor, but the best growth in liquid culture wort, wort culture as tea Zi sub leaflike layer fungus seed culture and liquid fermentation liquid for liquid; in shaking flask, three terpene content of not less than 1%, the content of polysaccharide in the above 15%. The biomass of seventh days to reach the maximum, while the HPLC measured the amount received most. With the extension of adenosine fermentation time, tea Zi sub leaflike layer fungus fungus ball color gradually from white to brown, and the fermentation liquid color gradually turned into brown, and have a fresh fruit flavor in this research. Using tissue isolation method of phallus hadriani bacteria strains isolated and molecular biological identification shows that isolated from mycelium as phallus hadriani. Phallus hadriani in different solid medium were used in the growth of wheat bran, malt juice, carrot, PDA solid medium growth is good, supporting adaptability for carbon powder stinkhorn and the nitrogen source is better in different carbon source, nitrogen source conditions, the mycelium growth rate and mycelium morphology are quite different. The screening medium of liquid fermentation by different medium and different carbon source and nitrogen source showed that PDA culture medium is suitable as a phallus hadriani seed in medium. Fermentation tank feed 7L, 10L sucrose 140g, soybean peptone 42g, yeast extract 70g, inoculum 5%, 26 degrees Celsius temperature, ventilation speed 150rpm, 1:0.3, 58h culture to fit tank. 18.02%. suitable for judging draw-off biaoda polysaccharide content in mycelium Standard index is the total sugar content of mycelia biomass and the fermentation liquid. The skirt of dictyophoraindusiata different culture could grow, in GPY, PDA, malt, wheat bran, carrots grown on solid medium is better in GPY solid medium, the fastest growth rate, the highest biomass and adaptability of 145.50mg. skirt Dictyophora of carbon source and organic nitrogen source is very strong. Dictyophora duplicata liquid seed culture medium for PDA medium in the fermentation tank feed 7L 10L, sucrose 140g, soybean peptone 42g, yeast extract 70g, inoculum 5%, 26 degrees Celsius temperature, ventilation speed 150rpm, 1:0.2, to 90h for training put the tank. 17.92%. content of polysaccharide in mycelium

【學(xué)位授予單位】：魯東大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：S646

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