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  本文關(guān)鍵詞： 玉米 全膜覆蓋 cDNA-AFLP 差異表達(dá)　出處：《內(nèi)蒙古大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：為了研究玉米苗期在全膜覆蓋條件下,基因的差異表達(dá)。本實(shí)驗(yàn)利用cDNA-AFLP技術(shù)對全膜覆蓋條件下種植的玉米與不覆膜條件下種植的玉米,進(jìn)行差異表達(dá)基因片段的篩選、分離、測序和功能性分析,結(jié)果如下:1.建立適合玉米苗期根系和葉片總RNA提取的方法,并將總RNA經(jīng)過反轉(zhuǎn)錄形成雙鏈cDNA。使用PstI和MseI兩種內(nèi)切酶對雙鏈cDNA進(jìn)行酶切,設(shè)計(jì)與兩種內(nèi)切酶相對應(yīng)的接頭以及9對選擇性擴(kuò)增引物,進(jìn)行特異片段擴(kuò)增及測序。2.運(yùn)用cDNA-AFLP技術(shù),共擴(kuò)增出條帶1481條,336條差異表達(dá)片段,約占總條帶數(shù)的22.7%。隨后將其中的60條進(jìn)行測序得到58條測序結(jié)果。3.利用NCBI系統(tǒng)對58條測序結(jié)果進(jìn)行同源性分析和功能預(yù)測。結(jié)果如下:與植物同源性較高的有13條并且大多具有蛋白功能;與動物同源性較高的有6條;與微生物同源性較高的有20條;另有,19條差異表達(dá)片段未能找到同源性序列。4.獲得的與植物同源性較高的13條基因片段中,有3條與小麥、高粱、柑桔植物體中相關(guān)功能基因高度相似,其余10條均與玉米體內(nèi)某些克隆基因高度相似。以上這些差異條帶的獲得為更深層次研究玉米基因在覆膜條件下的差異表達(dá)打下了良好的基礎(chǔ)。5.測序結(jié)果中獲得了 20條與微生物同源性高度相似的基因片段,這些基因片段的獲得以及在玉米生長過程中的作用還需進(jìn)一步研究確定。
[Abstract]:In order to study the differential expression of genes in seedling stage of maize under the condition of full film mulching, cDNA-AFLP technique was used to treat maize planted under the condition of full film mulching and maize without film mulching. Screening, isolation, sequencing and functional analysis of differentially expressed gene fragments were carried out. The results were as follows: 1. A suitable method for extracting total RNA from roots and leaves of maize at seedling stage was established. The total RNA was reversed to form double-stranded cDNA.The double strand cDNA was digested by PstI and MseI endonuclease. The specific fragments were amplified and sequenced by 9 pairs of primers. 1 481 bands were amplified by cDNA-AFLP technique. 336 differentially expressed fragments. Then 60 of them were sequenced to get 58 sequencing results .3.The homology analysis and functional prediction of 58 sequencing results were carried out by using NCBI system. The results were as follows:. Below:. 13 of them had high homology with plants and most of them had protein function. Six of them had high homology with animals. There were 20 high homology with microorganisms. Another 19 differentially expressed fragments could not find the homologous sequence. 4. Of the 13 genes with high homology with plants, 3 were with wheat and sorghum. The related functional genes in citrus plants are highly similar. The other 10 genes were highly similar to some cloned genes in maize. The above results laid a good foundation for further research on the differential expression of maize genes under the condition of film mulching. 5. Sequencing results. In the. There were 20 gene fragments with high homology with microorganism. The acquisition of these gene fragments and their roles in maize growth need to be further studied.
【學(xué)位授予單位】：內(nèi)蒙古大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：S513

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 汪順生;費(fèi)良軍;孫景生;高傳昌;傅渝亮;;控制性交替隔溝灌溉對夏玉米生理特性和水分生產(chǎn)效率的影響[J];干旱地區(qū)農(nóng)業(yè)研究;2011年05期

2 朱玉廷;;玉米單產(chǎn)900千克栽培技術(shù)模式[J];現(xiàn)代農(nóng)業(yè);2011年09期

3 楊繼芝;龔國淑;張敏;陳華保;王學(xué)貴;楊春平;;密度和品種對玉米田雜草及玉米產(chǎn)量的影響[J];生態(tài)環(huán)境學(xué)報(bào);2011年Z1期

4 高鴻永;王果芝;陳愛萍;趙秀蘭;;河套地區(qū)覆膜玉米土壤溫度與含水量對作物影響的研究[J];內(nèi)蒙古水利;2011年01期

5 李尚中;王勇;樊廷錄;王立明;趙剛;唐小明;黨翼;王磊;張建軍;;旱地玉米不同覆膜方式的水溫及增產(chǎn)效應(yīng)[J];中國農(nóng)業(yè)科學(xué);2010年05期

6 杜紅霞;吳普特;馮浩;王百群;馬軍勇;;氮施用量對夏玉米土壤水氮動態(tài)及水肥利用效率的影響[J];中國水土保持科學(xué);2009年04期

7 張軍錢;;旱地全膜雙壟溝播玉米適宜覆膜時間研究[J];水土保持通報(bào);2009年04期

8 孫學(xué)保;楊祁峰;�？×x;張文斌;牛芬菊;;旱地全膜雙壟溝播玉米增產(chǎn)效應(yīng)研究[J];作物雜志;2009年03期

9 李來祥;劉廣才;楊祁峰;趙小文;朱永永;;甘肅省旱地全膜雙壟溝播技術(shù)研究與應(yīng)用進(jìn)展[J];干旱地區(qū)農(nóng)業(yè)研究;2009年01期

10 劉廣才;楊祁峰;李來祥;樊廷錄;趙小文;朱永永;;旱地玉米全膜雙壟溝播技術(shù)土壤水分效應(yīng)研究[J];干旱地區(qū)農(nóng)業(yè)研究;2008年06期

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