本文關(guān)鍵詞： 羅非魚 復(fù)合益生菌 基因表達(dá) 免疫保護(hù)率　出處：《湖南農(nóng)業(yè)大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

【摘要】：本研究通過把三種復(fù)合益生菌添加到飼料中,分別飼喂羅非魚(Tilapia),確定提升免疫保護(hù)率最佳的復(fù)合益生菌種類,并從分子水平初步探索復(fù)合益生菌對(duì)羅非魚免疫力影響的作用機(jī)理。試驗(yàn)共分4組,3個(gè)試驗(yàn)組和1個(gè)對(duì)照組。3個(gè)試驗(yàn)組用分別添加了等比例的地衣芽孢桿菌(Bacillus licheniformis)+枯草芽孢桿菌(Bacillus subtilis)、地衣芽孢桿菌(Bacillus licheniformis)+凝結(jié)芽孢桿菌(Bacillus coagulans)、地衣芽孢桿菌(Bacillus licheniformis)+糞腸球菌(Enterococcus faecalis)的飼料飼喂羅非魚,添加量為2‰。飼喂40天,期間每隔10天取羅非魚肝臟、腎臟、頭腎、脾臟以及鰓五個(gè)器官,采用實(shí)時(shí)熒光定量PCR技術(shù)檢測各器官中環(huán)氧化酶-2(COX-2)、白細(xì)胞介素-1β(IL-1β)和腫瘤壞死因子-a (TNF-a)這三種免疫相關(guān)基因的相對(duì)表達(dá)量變化情況。40天后,以攻毒試驗(yàn)來檢測復(fù)合益生菌對(duì)羅非魚抗感染保護(hù)率的影響。分別給羅非魚胸鰭基部注射嗜水氣單胞菌、維氏氣單胞菌和無乳鏈球菌三種羅非魚常見致病菌。試驗(yàn)結(jié)果表明：三種不同的復(fù)合益生菌都顯著(P0.05)誘導(dǎo)羅非魚五個(gè)器官中CoX-2、IL-1β和TNF-a基因的表達(dá),其中地衣枯草組誘導(dǎo)表達(dá)效果最明顯；基因誘導(dǎo)表達(dá)在免疫器官腎臟、頭腎和脾臟中要比非免疫器官肝和鰓中明顯；地衣枯草組提高了羅非魚對(duì)嗜水氣單胞菌(Aeromonas hydrophila)、維氏氣單胞菌(Aeromonas veronii)和無乳鏈球菌(Streptococcus agalactiae)的抗感染保護(hù)率；地衣凝結(jié)組提高了羅非魚對(duì)嗜水氣單胞菌和維氏氣單胞菌的抗感染保護(hù)率。
[Abstract]:In this study, three kinds of compound probiotics were added to feed tilapia tilapia Tilapia to determine the best compound probiotics to enhance the immune protection rate. The mechanism of the effect of compound probiotics on the immunity of tilapia was preliminarily explored at the molecular level. The experiment was divided into 4 groups. Three experimental groups and one control group were treated with Bacillus licheniformis (Bacillus licheniformis). Bacillus subtilis. Bacillus licheniformis (Bacillus coagulans). The feed of Bacillus licheniformis, Enterococcus faecalis, was fed to tilapia. The dosage was 2 鈥，

本文編號(hào)：1446146