本文關(guān)鍵詞： 白菜 抽薹性狀 單片段代換系 上位性　出處：《沈陽農(nóng)業(yè)大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：抽薹性狀是白菜的重要農(nóng)藝性狀之一。上位性效應(yīng)是由非等位基因間相互作用產(chǎn)生的,也是復(fù)雜數(shù)量性狀重要的組份,同時(shí)對白菜的抽薹性狀具有重要影響。本研究以篩選的5個(gè)染色體單片段代換系和輪回親本'RcBr'為試材,通過半輪配法構(gòu)建10個(gè)雙片段聚合系為試驗(yàn)材料,對抽薹相關(guān)QTL間的上位性效應(yīng)進(jìn)行分析。研究結(jié)果如下:1.本研究以F2群體,RIL群體和CSSLs群體的抽薹相關(guān)性狀的QTL定位結(jié)果為參考,篩選出14個(gè)染色體片段代換系。以初步篩選出14個(gè)染色體片段代換系為試材,結(jié)合株系的表現(xiàn)型和基因型,進(jìn)行驗(yàn)證試驗(yàn)。最終選取5個(gè)單片段代換系對上位性互作效應(yīng)進(jìn)行分析。每個(gè)試驗(yàn)材料均攜帶控制抽薹相關(guān)性狀的QTL位點(diǎn),其中有2個(gè)QTLs位點(diǎn)控制抽薹指數(shù),均位于A07染色體上;其余3個(gè)QTLs位點(diǎn)控制開花時(shí)間,分別位于A02、A07和A08染色體上。2.本研究以歐洲白菜型油菜'RcBr'為受體親本構(gòu)建的帶有抽薹相關(guān)性狀QTL的5個(gè)單片段代換系為試驗(yàn)材料,經(jīng)兩兩雜交,通過分子標(biāo)記輔助選擇(Marker-assisted selection,MAS),篩選出10個(gè)不同親本組合的雙片段聚合系。聚合系WD3(16swd001/16swd007):代換系16swd001攜帶的qFT-2-1對代換系16swd007攜帶的qFT-7-1表現(xiàn)上位性。聚合系WD4(16swd001/16swd009):代換系16swd001攜帶的qFT-2-1對代換系16swd009攜帶的qFT-8-1表現(xiàn)上位性。聚合系WD10(16swd007/16swd009):將聚合系WD10的開花時(shí)間與兩親本16swd007和16swd009的開花時(shí)間進(jìn)行比較,發(fā)現(xiàn)開花時(shí)間未達(dá)到統(tǒng)計(jì)學(xué)顯著差異。因此,可以判斷兩親本攜帶的2個(gè)QTLs位點(diǎn)之間沒有相互的疊加效應(yīng),但無法判斷是否存在互作效應(yīng)。3.與本研究相同親本構(gòu)建的RIL群體抽薹上位性效應(yīng)鑒定結(jié)果比較,兩個(gè)試驗(yàn)均檢測到控制抽薹相關(guān)性狀QTL位點(diǎn)之間存在上位性效應(yīng),但是檢測到存在上位性效應(yīng)的位點(diǎn)不同。本研究共檢測出2對控制開花時(shí)間的QTLs位點(diǎn)之間存在上位性效應(yīng),分別是 qFT-2-1 與 qFT-7-1 和 qFT-2-1 與 qFT-8-1。
[Abstract]:As one of the important agronomic traits of Chinese cabbage, the epistatic effect is produced by the interaction of non-alleles and is also an important component of complex quantitative traits. At the same time, it has an important effect on the characters of Chinese cabbage. In this study, five chromosome single fragment substitution lines and their recurrent parents, RcBr', were selected as the test materials. Ten two-segment polymerization lines were constructed by half-wheel matching method to analyze the epistatic effect among QTL. The results are as follows: 1. In this study, F2 population was used. For reference, 14 chromosome fragments were selected from RIL population and CSSLs population, and 14 chromosome fragment substitution lines were selected as test materials. Finally, five single fragment substitution lines were selected to analyze the epistatic interaction effect. Each of the tested materials carried the QTL locus which controlled the characteristics of coating. Among them, 2 QTLs loci controlled the sprouting index, all of which were located on chromosome A07. The other three QTLs loci controlled blooming time, respectively, at A02. Chromosomes A07 and A08. In this study, five single-fragment substitution lines with QTL, which were derived from Brassica campestris L. as recipient parent, were used as experimental materials. After pairwise hybridization, Marker-assisted selection was selected by molecular marker-assisted selection (MAS). Ten two-fragment aggregates of different parent combinations were screened. The aggregation line WD3A16swd001 / 16swd007). : the qFT-2-1 carried by the substitution line 16swd001 is epistatic to the qFT-7-1 carried by the substitution line 16swd007. 16swd001 / 16SWd009). : the qFT-2-1 carried by the substitution line 16swd001 is epistatic to the qFT-8-1 carried by the substitution line 16swd009. 16swd007 / 16swd009: the blooming time of WD10 was compared with that of two parents 16swd007 and 16swd009. It was found that the blooming time was not statistically significant. Therefore, it could be judged that there was no superposition effect between the two QTLs loci carried by the two parents. But it is impossible to judge whether there is interaction effect. 3. Compared with the result of epistatic effect of RIL population constructed by the same parents in this study. The epistatic effect was detected between QTL loci of the traits related to the control of bolting in both experiments. However, different loci with epistatic effect were detected. In this study, two pairs of QTLs loci controlling blooming time were detected to have epistatic effect. They are qFT-2-1 and qFT-7-1 and qFT-2-1 and qFT-8-1, respectively.
【學(xué)位授予單位】：沈陽農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：S634.1

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