本文關(guān)鍵詞：甲基乙二醛在圍產(chǎn)期奶牛酮病群體監(jiān)測(cè)中的作用　出處：《西北農(nóng)林科技大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 圍產(chǎn)期奶牛 群體監(jiān)測(cè) 酮病 甲基乙二醛 磷酸二羥丙酮

【摘要】：圍產(chǎn)期奶牛酮病作為重要的多發(fā)性和群發(fā)性疾病,為乳業(yè)造成了嚴(yán)重的經(jīng)濟(jì)損失。因此,對(duì)圍產(chǎn)期奶牛酮病進(jìn)行群體監(jiān)測(cè)和早期預(yù)警顯得尤為重要。甲基乙二醛(methylglyoxal,MGO)作為一個(gè)高活性α-氧代醛,在糖尿病及其并發(fā)癥中起著重要作用,被認(rèn)為是疾病發(fā)展的一個(gè)生物標(biāo)志物。酮病奶牛血液中是否能夠產(chǎn)生MGO,其在代謝中發(fā)揮什么作用?目前仍不清楚。為明確血清MGO是否與奶牛酮病有相關(guān)性,本論文通過檢測(cè)奶牛血清中糖、脂質(zhì)、礦物元素代謝等相關(guān)指標(biāo),以及日糧營(yíng)養(yǎng)水平和生產(chǎn)性能等指標(biāo),篩選和確定圍產(chǎn)期奶牛酮病群體監(jiān)測(cè)的預(yù)警體系,探討血清MGO在奶牛酮病群體監(jiān)測(cè)中的作用。結(jié)果如下:從產(chǎn)前2周開始,圍產(chǎn)期奶牛血清中BHBA、NEFA和AST濃度逐漸升高,產(chǎn)后1周(第7 d)達(dá)到最高,此后逐漸降低;血清中GLU、Ca、K和P濃度從產(chǎn)前2周開始逐漸降低,產(chǎn)后1周最低,此后逐漸恢復(fù);圍產(chǎn)期奶牛,產(chǎn)前BCS和血清中GLU、Mg和K與產(chǎn)后水平呈顯著正相關(guān);泌乳早期(產(chǎn)后第14 d),圍產(chǎn)期奶牛血清中NEFA與牛如中MF、F/P呈顯著正相關(guān),與MP呈顯著負(fù)相關(guān);GLU與日糧中DM、EE、CF、NDF、ADF和MUN呈顯著負(fù)相關(guān),與GE呈顯著正相關(guān);BCS與MF、ML和TS呈顯著正相關(guān),與日糧中DM、EE、CF、NDF和ADF呈顯著負(fù)相關(guān);干奶天數(shù)與產(chǎn)后1周血清中BHBA和NEFA濃度呈顯著正相關(guān)。泌乳早期,與健康奶牛相比,酮病奶牛干奶天數(shù),血清中BHBA、NEFA,牛乳中F/P顯著增加,MP顯著降低。酮病奶牛血清中MGO濃度顯著高于青年奶牛和健康奶牛;與青年奶牛相比,酮病奶牛血清中GLU、FA、TG、K、P和Mg濃度均顯著降低,DHAP、NEFA、BHBA、AST、TBIL和Hp含量顯著升高;試驗(yàn)?zāi)膛Ｑ逯蠳EFA與GLU、FA、K、P和Mg濃度呈顯著負(fù)相關(guān),與AST、TBIL、Hp和DHAP顯著正相關(guān);BHBA與GLU、FA、TG、Mg和K呈顯著負(fù)相關(guān),與AST、TBIL、Hp和DHAP顯著正相關(guān);MGO與GLU、FA、TG、K、P和Mg呈顯著負(fù)相關(guān),與BHBA、NEFA、AST、TBIL、Hp和DHAP呈顯著正相關(guān);泌乳奶牛血清中MGO與GLU和FA呈顯著負(fù)相關(guān),與BHBA、NEFA、TG、Hp和P呈顯著正相關(guān)。結(jié)論:泌乳早期,血清中BHBA和NEFA可作為監(jiān)測(cè)酮病的首選指標(biāo),血清中GLU、AST,BCS,MF、MP和F/P可作為監(jiān)測(cè)酮病的輔助指標(biāo)。酮病奶牛血清中MGO產(chǎn)生的增加,可能來源為脂質(zhì)代謝產(chǎn)生的酮體和甘油糖異生生成的磷酸二羥丙酮;MGO可作為奶牛酮病群體監(jiān)測(cè)中的一個(gè)生物標(biāo)志物。
[Abstract]:Perinatal ketosis in dairy cows, as an important multiple and mass disease, has caused serious economic losses for the dairy industry. Group monitoring and early warning of perinatal ketosis in dairy cattle is particularly important. Methyl Glyoxal methylglyoxalMGOA is a highly active 偽 -oxaldehyde. It plays an important role in diabetes mellitus and its complications and is considered to be a biomarker of disease development. Can MGOs be produced in the blood of ketosis cows and what role does MGOs play in metabolism? It is still unclear. In order to determine whether serum MGO is related to ketosis in dairy cows, this paper detected the serum glucose, lipid, mineral elements metabolism and other related indicators. As well as dietary nutrition level and production performance indicators to screen and determine the perinatal cow ketodisease monitoring early warning system. To explore the role of serum MGO in the monitoring of ketopathy in dairy cattle. The results are as follows: from the second week before delivery, the serum concentration of MGO and AST in the perinatal dairy cattle gradually increased. The peak was reached at 1 week postpartum (7 d), and then decreased gradually. The concentrations of GLU CaK and P in serum decreased gradually from 2 weeks before delivery to the lowest in 1 week postpartum, and then recovered gradually. In perinatal dairy cows, BCS and GLU mg and K in serum were positively correlated with postpartum levels. At the early stage of lactation (postpartum 14th day), the serum NEFA in perinatal cows was positively correlated with that in cattle such as MFF / P, and negatively correlated with MP. There was a significant negative correlation between GLU and ADF and MUN, and a significant positive correlation between GLU and GE. There was a significant positive correlation between BCS and MFML and TS, and a significant negative correlation between BCS and CFD and ADF in diet. The days of dry milk were positively correlated with the concentration of BHBA and NEFA in serum at 1 week postpartum. The concentration of MGO in serum of ketosis cows was significantly higher than that of young cows and healthy cows. Compared with the young cows, the serum concentrations of GLU / FAA, TGG, P and mg in ketosis cows were significantly lower than those in young cows. The contents of TBIL and HP increased significantly. There was a significant negative correlation between serum NEFA and GLU FAKP and mg concentration, and a positive correlation between NEFA and TBILHP and DHAP. BHBA was negatively correlated with mg and K, and positively correlated with HP and DHAP. There was a significant negative correlation between MGO and TGG P and mg, and a significant positive correlation between MGO and HP and DHAP. There was a significant negative correlation between serum MGO and GLU and FA in lactating cows, and a significant positive correlation with HP and P in NEFAA. Conclusion: early lactation. Serum BHBA and NEFA can be used as the first choice for monitoring ketosis. MP and F / P can be used as auxiliary indexes for monitoring ketosis. The increase of MGO production in serum of ketosis cows may come from the ketone body produced by lipid metabolism and dihydroxyacetone phosphate produced by glycerol glycometabolism. MGO can be used as a biomarker for monitoring of ketosis in dairy cattle.
【學(xué)位授予單位】：西北農(nóng)林科技大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：S858.23

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相關(guān)期刊論文 前10條

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本文編號(hào)：1423752