本文關(guān)鍵詞：沙蔥水溶性提取物對肉羊脂肪代謝相關(guān)基因表達(dá)量及甲基化的影響　出處：《內(nèi)蒙古農(nóng)業(yè)大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 沙蔥水提物 肉羊 甲基化 mRNA表達(dá) LPL PPARγ

【摘要】：課題組前期的研究中發(fā)現(xiàn),沙蔥及其提取物可以降低肉羊機(jī)體內(nèi)脂肪酸合成酶的活性,提高激素敏感脂酶的活性,從而影響舍飼肉羊胴體脂肪的分布和脂肪中脂肪酸的組成,并顯著改善羊肉風(fēng)味和品質(zhì)。通過在肉羊飼料中添加沙蔥水溶性提取物,研究沙蔥水提物對脂蛋白脂酶(Lipoprotein Lipase,LPL),過氧化物酶體增殖體激活受體(Peroxisome proliferator-activated receptors,PPAγ)基因mRNA表達(dá)和啟動(dòng)子甲基化程度的影響。試驗(yàn)選取24只羊隨機(jī)分為2組[對照組(基礎(chǔ)日糧組)和試驗(yàn)組(基礎(chǔ)日糧中添加0.1%沙蔥水溶性提取物)],每組12只羊體重相近。預(yù)飼期15d,正飼期60d。正式試驗(yàn)結(jié)束后,每組隨機(jī)選取6只樣本屠宰,立即采集肉羊背部脂肪組織,進(jìn)行Q-PCR測定LPL,PPARγ基因mRNA表達(dá)量。經(jīng)RNA電泳圖,擴(kuò)增曲線及溶解曲線驗(yàn)證,樣品結(jié)果符合試驗(yàn)要求,LPL和PPARγmRNA表達(dá)較對照組均有極顯著差異(P0.001),LPL較對照組降低35%,相反PPAR γ較對照組增加35%。經(jīng)篩選LPL基因中有4個(gè)高甲基化CpG島記為LPLM1,LPLM2,LPLM3,LPLM4。PPARγ有一個(gè)高甲基化CpG島。通過測序錯(cuò)誤率分布驗(yàn)證以及原始數(shù)據(jù)堿基組成分布驗(yàn)證后分析LPL和PPARγ基因甲基化比例發(fā)現(xiàn),在LPLM2中161dp試驗(yàn)組甲基化比例和LPLM3中32dp、57dp試驗(yàn)組甲基化比例顯著高于對照組,分別上升32.0%,62.3%,51.9%,其LPL各位點(diǎn)的平均甲基化比例顯著提高了 21.4%,但PPARγ甲基化比例試驗(yàn)組與對照組之間并無顯著差異。
[Abstract]:In our previous study, it was found that Allium mongolicum and its extract could reduce the activity of fatty acid synthase and increase the activity of hormone sensitive lipase in meat sheep. Thus, the distribution of carcass fat and fatty acid composition in meat sheep were affected, and the flavor and quality of mutton were improved significantly. To study the effect of water extract from Allium sativa on lipoprotein Lipase (LPL). Peroxisome proliferator-activated receptors. The effects of PPA 緯) gene mRNA expression and promoter methylation were studied. 24 sheep were randomly divided into 2 groups. [The control group (basal diet group) and experimental group (basic diet supplemented with 0.1% water soluble extract of Allium balsica)], each group of 12 sheep body weight, 15 days prefeeding period, 60 days, after the formal trial. Six samples were slaughtered randomly in each group. The fat tissue of back of sheep was collected immediately and the expression of LPLPPAR- 緯 gene mRNA was determined by Q-PCR. RNA electrophoresis was used to detect the expression of LPLPPAR- 緯 gene. The results of amplification curve and dissolution curve showed that the expression of LPL and PPAR 緯 mRNA was significantly different from that of the control group (P0.001). Compared with the control group, LPL decreased 35%, whereas PPAR 緯 increased 35%. Four hypermethylated CpG islands in the screened LPL gene were recorded as LPLM1 / LPLM2. LPLM3. LPLM4.PPAR 緯 has a highly methylated CpG island. LPL and PPAR 緯 gene methylation ratios were analyzed by sequencing error rate distribution and base composition distribution of the original data. The methylation rate of the 161dp test group in LPLM2 and the methylation rate of the 32dp57-dp test group in LPLM3 was significantly higher than that of the control group, with a rise of 32.0% and 62.3%, respectively. The average methylation ratio of the LPL sites increased by 21.4g, but there was no significant difference between the PPAR 緯 methylation ratio test group and the control group.
【學(xué)位授予單位】：內(nèi)蒙古農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：S826.5

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