本文關(guān)鍵詞：丁香酸影響舞毒蛾LdNPV致死與亞致死作用及其機制　出處：《內(nèi)蒙古農(nóng)業(yè)大學》2017年碩士論文　論文類型：學位論文

  更多相關(guān)文章： 舞毒蛾 LdNPV 丁香酸 致病力 亞致死作用

【摘要】：研究丁香酸(syringicacid,SY)對舞毒蛾核型多角體病毒(LdNPV)致死及亞致死作用的影響,為舞毒蛾LymantriadisparLL的防治提供科學依據(jù)。采用食料給毒法進行生物測定,評估LdNPV及SY與LdNPV共同對舞毒蛾2齡幼蟲的致死和亞致死作用,研究結(jié)果如下:1.取食添加丁香酸人工飼料和正常人工飼料的舞毒蛾幼蟲LdNPV致死中濃度(LC50)分別為 91.1 OBs/μl 和 194.8 OBs/μl。當 LdNPV 濃度為 2 400 OBs/μl,取食添加SY的飼料及正常人工飼料的舞毒蛾幼蟲LdNPV致死中時間(LT50)分別為9 d 和 10.1 d,當 LdNPV 濃度為 24 000 OBs/μl 時,其 LT50 分別為 7.8 d 和 8.1 d,LdNPV的濃度越高致死中時間越短。丁香酸增加了 LdNPV對舞毒蛾幼蟲的致病力,降低了LdNPV對舞毒蛾幼蟲的致死中濃度,縮短了致死中時間。2.LdNPV對取食正常飼料的雌、雄幼蟲歷期和蛹期及雌蛾壽命影響不顯著,但顯著縮短了雄蟲壽命,降低了平均每雌產(chǎn)卵量。LdNPV對取食添加丁香酸飼料的幼蟲產(chǎn)生明顯的亞致死效應,并且LdNPV濃度較高時雌、雄幼蟲歷期延長、雄蛾壽命縮短,雄性羽化率降低,雌成蟲產(chǎn)卵量降低,產(chǎn)卵量較對照減少一半左右。3.酚氧化酶在單獨正常飼料(CK)處理時,隨著時間其活力逐漸增加。單獨丁香酸飼料處理時在前60h酚氧化酶活力隨時間升高,且高于對照(CK),84h時降到最低,且顯著低于對照。單獨病毒處理后的舞毒蛾酚氧化酶活性在36h后受到抑制,活力降低。過氧化物酶在處理前期酶活性受到了抑制,后期單獨正常處理的舞毒蛾酶活性降低和其他處理的酶活性差異趨于平緩。
[Abstract]:To study the effects of syringic acid syringic acid syringe on the lethal and sublethal effects of LdNPV. To provide scientific basis for the prevention and control of LymantriadisparLL, the bioassay was carried out by the method of feeding food with poison. To evaluate the lethal and sublethal effects of LdNPV, sy and LdNPV on the 2nd instar larvae. The results are as follows: 1. LdNPV lethal median concentration (LC50) of moth larvae fed with eugenic acid artificial diet and normal artificial diet was obtained. The concentrations of LdNPV were 91.1 OBs / 渭 l and 194.8 OBs / 渭 l, respectively, when the concentration of LdNPV was 2 400 OBs / 渭 l. The median LdNPV lethal time (LT50) of moth larvae fed on diet supplemented with sy and normal artificial diet was 9 days and 10. 1 days, respectively. When the concentration of LdNPV was 24 000 OBs / 渭 l, its LT50 was 7.8 d and 8.1 d, respectively. The higher the concentration of LdNPV, the shorter the median lethal time. Eugenic acid increased the pathogenicity of LdNPV to moth larvae and decreased the median lethal concentration of LdNPV to moth larvae. LdNPV shortened the median lethal time. 2. LdNPV had no significant effect on female, male larva duration, pupa stage and female moth longevity, but significantly shortened male life span. LdNPV decreased the average number of eggs laid per female. LdNPV had obvious sublethal effect on larvae fed with eugenic acid feed, and the duration of male and female larvae was prolonged and the lifespan of male moth was shortened when the concentration of LdNPV was higher. The emergence rate of male decreased, the oviposition of female adult decreased, and the oviposition of female adult decreased by about half compared with the control. Phenol oxidase was treated with CK alone. The activity of phenoloxidase increased with time during the first 60 hours of treatment with eugenic acid, and decreased to the lowest level when compared with CK at 84 h. The activity of phenoloxidase was inhibited and the activity of peroxidase was decreased after 36 h treatment, and the activity of peroxidase was inhibited in the early stage of treatment. The difference of enzyme activity between the two treatments and that of other treatments tended to be flat.
【學位授予單位】：內(nèi)蒙古農(nóng)業(yè)大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：S763.42

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