本文選題：�；撬� + 下丘腦-垂體-卵巢軸��； 參考：《沈陽農(nóng)業(yè)大學(xué)》2015年博士論文

【摘要】：牛磺酸作為人和動(dòng)物的條件性必需氨基酸,是雌性動(dòng)物生殖腺和生殖細(xì)胞中含量最為豐富的游離氨基酸之一,已有研究證明�；撬崤c卵巢組織發(fā)育成熟及雌激素合成分泌密切相關(guān),但其具體機(jī)制尚不清楚。本研究以雌性大鼠為試驗(yàn)動(dòng)物,從mRNA和蛋白水平檢測了�；撬嵘锖铣申P(guān)鍵酶—半胱氨酸雙加氧酶(CDO)、半胱亞磺酸脫羧酶(CSD)及�；撬徂D(zhuǎn)運(yùn)體(TAUT)在下丘腦-垂體-卵巢軸中的表達(dá)；通過�；撬犸曃乖囼�(yàn),檢測了牛磺酸對(duì)不同發(fā)情周期大鼠生殖激素分泌的影響；采用體外細(xì)胞培養(yǎng)技術(shù),檢測了牛磺酸對(duì)體外培養(yǎng)大鼠下丘腦細(xì)胞、垂體細(xì)胞、卵巢細(xì)胞激素分泌及相關(guān)因子表達(dá)的影響；通過體外培養(yǎng)卵泡和卵母細(xì)胞檢測了牛磺酸對(duì)卵泡發(fā)育、卵母細(xì)胞成熟、受精及卵裂的影響,探討了�；撬釋�(duì)雌性生殖機(jī)能作用的影響及其可能機(jī)制,以期為牛磺酸在女性及雌性動(dòng)物生殖中的應(yīng)用提供理論依據(jù)。研究結(jié)果如下：1.成功克隆出了大鼠下丘腦-垂體-卵巢軸上CSD基因的編碼區(qū)序列,下丘腦CDO基因的編碼區(qū)序列,卵巢CDO基因的全長序列及下丘腦-垂體-卵巢軸上TAUT基因的全長序列,且與肝臟中基因的核苷酸序列、氨基酸序列均有較高同源率(96%以上)；下丘腦-垂體-卵巢軸上也存在CSD、CDOTAUT蛋白表達(dá)；CDO、CSDTAUT主要分布于卵泡內(nèi)膜間質(zhì)細(xì)胞層。結(jié)果表明：下丘腦、垂體和卵巢能通過CSD通路合成牛磺酸,并具有轉(zhuǎn)運(yùn)外源�；撬崛氚哪芰Α�2.�；撬釋�(duì)不同發(fā)情周期大鼠促性腺激素釋放激素(GnRH)、泌乳素(PRL)、促黃體生成素(LH)、雌二醇(E2)和孕酮(P)的分泌均有一定的刺激作用,但對(duì)促卵泡刺激素(FSH)分泌的影響不明顯。結(jié)果表明：牛磺酸可能通過作用于下丘腦-垂體-卵巢軸來調(diào)控雌性大鼠的生殖機(jī)能。3.牛磺酸能促進(jìn)體外培養(yǎng)大鼠下丘腦細(xì)胞GnRH、腺垂體細(xì)胞PRL和LH、卵巢內(nèi)膜細(xì)胞T、顆粒細(xì)胞E2和P的分泌；提高內(nèi)膜細(xì)胞中促黃體生成素受體(LHR)、3β-羥基類固醇脫氫酶(3p-HSD)和17p-羥基類固醇脫氫酶(17P-HSD) mRNA的表達(dá),增加顆粒細(xì)胞中促卵泡刺激素受體(FSHR)和CYP-19A-1 mRNA的表達(dá)。結(jié)果表明：�；撬峥赏ㄟ^增加卵巢中FSHR和LHR的數(shù)量,促進(jìn)性激素合成關(guān)鍵酶的表達(dá)來促進(jìn)雌激素的合成和分泌。4.�；撬峥纱龠M(jìn)離體培養(yǎng)卵泡直徑增長和E2的分泌；�；撬釋�(duì)離體培養(yǎng)卵母細(xì)胞MⅡ期形成、受精及卵裂具有促進(jìn)作用。結(jié)果表明：牛磺酸能促進(jìn)卵泡發(fā)育和卵母細(xì)胞成熟、受精及卵裂率。本文試驗(yàn)結(jié)果表明,大鼠下丘腦-垂體-卵巢軸均可生物合成�；撬�,而且具有將外源牛磺酸轉(zhuǎn)運(yùn)入胞的能力；牛磺酸可通過增強(qiáng)下丘腦-垂體-卵巢軸的功能來調(diào)控雌性生殖機(jī)能。
[Abstract]:Taurine is one of the most abundant free amino acids in the gonads and germ cells of female animals. Studies have shown that taurine is closely related to ovarian tissue maturation and estrogen synthesis and secretion, but its mechanism is not clear. In this study, the expression of cysteine dioxygenase (cysteine dioxygenase), cysteine decarboxylase (cysteine decarboxylase) and taurine transporter (taurine) in hypothalamus-pituitary-ovary axis was detected from mRNA and protein levels in female rats. The effects of taurine on the secretion of reproductive hormones in rats with different estrous cycles were tested by taurine feeding test, and the effects of taurine on rat hypothalamic and pituitary cells in vitro were detected by cell culture technique in vitro. The effects of taurine on follicle development, oocyte maturation, fertilization and cleavage were examined by in vitro culture of follicles and oocytes. The effect of taurine on female reproductive function and its possible mechanism were discussed in order to provide theoretical basis for the application of taurine in female and female animal reproduction. The results are as follows: 1. The coding region sequence of CSD gene on hypothalamus-pituitary-ovary axis, the coding region sequence of CDO gene in hypothalamus, the full-length sequence of CDO gene in ovary and the full-length sequence of TAUT gene on hypothalamus-pituitary-ovary axis were cloned successfully. Moreover, the nucleotide sequence and amino acid sequence of the gene in the liver had a high homology rate of more than 96%, and the expression of CSD- CDOTAUT protein on the hypothalamus-pituitary-ovary axis was mainly distributed in the interstitial layer of the follicular intima. The results showed that the hypothalamus, pituitary and ovary could synthesize taurine via CSD pathway and have the ability of transporting exogenous taurine into cells. Taurine stimulated the secretion of gonadotropin releasing hormone (GnRH), prolactin (PRL), luteinizing hormone (LH), estradiol (E 2) and progesterone (P) in rats with different estrus cycle, but had no obvious effect on follicle stimulating hormone (FSH) secretion. The results showed that taurine may regulate the reproductive function of female rats by acting on hypothalamus-pituitary-ovarian axis. Taurine could promote the secretion of GnRH in rat hypothalamic cells, PRL and LHin adenohypophysis cells, T, E 2 and P in granulosa cells. To increase the expression of luteinizing hormone receptor (LHR3 尾 -hydroxysteroid dehydrogenase 3p-HSD3) and 17p- hydroxysteroid dehydrogenase (17P-HSD) mRNA, and to increase the expression of follicle-stimulating hormone receptor (FSHR) and CYP-19A-1 mRNA in granulosa cells. The results showed that taurine could promote the synthesis and secretion of estrogen by increasing the number of FSHR and LHR in ovary and promoting the expression of key enzymes of sex hormone synthesis. Taurine could increase the diameter of follicle and secretion of E2 in vitro, and taurine could promote the formation of M 鈪，

本文編號(hào)：1864032