發(fā)布時間：2025-04-18 03:07

　　抗生素因具有選擇性抑制或殺滅其它菌種微生物的能力,而被廣泛應用于醫(yī)療、農業(yè)及畜牧養(yǎng)殖業(yè),尤其是隨著集約化畜牧業(yè)的發(fā)展,使用抗生素己成為保障畜牧業(yè)持續(xù)健康發(fā)展所必不可少的核心環(huán)節(jié)。由于科學知識的缺乏和經濟利益的驅使,養(yǎng)殖業(yè)成為了四環(huán)素、氨基糖苷等各類獸用抗生素濫用的重災區(qū)�？股貫E用的直接后果是導致其在動物源性食品中的殘留,后經食物鏈在人體內蓄積,引發(fā)過敏和變態(tài)反應、損傷神經系統(tǒng)、抑制骨髓造血機能、導致耐藥菌株感染等危害。當前,抗生素耐藥性細菌已蔓延至全球各地,如不采取行動對濫用進行干預,人類將“無藥可救”。世界衛(wèi)生組織也將抗生素濫用視為國際關注的重大問題之一,對相關重點抗生素的每日攝入量（Acceptable daily intake,ADI）和最大殘留限量（Maximum residue limit,MRL）做出了規(guī)定,以期管控動物源性食品及環(huán)境中的抗生素殘留。現(xiàn)有的抗生素檢測方法有高效液相色譜、毛細管電泳法、電化學分析儀及微生物分析法等,盡管上述方法靈敏度高、選擇性好且檢出限低,但也面臨儀器昂貴、檢測成本高、操作繁瑣及測定時間長等不足,無法充分滿足大批樣品的現(xiàn)場過篩需求。因此,研發(fā)靈...

【文章頁數(shù)】：267 頁

【學位級別】：博士

【文章目錄】：
ACKNOWLEDGEMENTS
DEDICATION
ABSTRACT
摘要
ABBREVIATION
CHAPTER1.INTRODUCTION AND LITERATURE REVIEW
    1.1.Introduction
    1.2.Overview of antibiotics
        1.2.1.Classification,structure,and application
        1.2.2.Status and hazards of antibiotics residues
        1.2.3.Measures to prevent antibiotics residues
    1.3.Traditional techniques for the detection of antibiotic residues
        1.3.1.HPLC
        1.3.2.Electrochemical analysis for antibiotics detection
        1.3.3.Ultraviolet(UV)detection methods
        1.3.4.Mass spectrometry(MS)
        1.3.5.Comparison of traditional antibiotic detection methods
    1.4.Colorimetric analysis and its application
        1.4.1.Overview of colorimetric analysis
        1.4.2.The basis of the colorimetric method
        1.4.3.Application of the colorimetric method in antibiotic detection
    1.5.Electrospun nanofibers and their application in sensing
        1.5.1.Introduction to electrospinning
        1.5.2.Nanostructure by electrospinning
        1.5.3.Application of electrospinning nanofibers in the sensor field
    1.6.Purpose and objective of the study
    1.7.Innovation points
    1.8.Limitation
    1.9.Background and research contents of the thesis
    1.10.References
CHAPTER2.OXYTETRACYCLINE STRIPS BASED ON NICKEL(II)IONS IMMOBILIZED NANOFIBROUS MEMBRANES
    2.1.Introduction
    2.2.Experimental
        2.2.1.Materials and reagents
        2.2.2.Preparation of PAN NFMs
        2.2.3.Surface modification of PAN NFMs
        2.2.4.Fabrication of colorimetric strips
        2.2.5.Detection of OTC
        2.2.6.Characterization
    2.3.Results and discussion
        2.3.1.Fabrication of colorimetric strips
        2.3.2.The optimization of detection conditions
        2.3.3.The sensitivity of colorimetric strips
        2.3.4.Selectivity and reversibility of colorimetric strips
    2.4.Chapter summary
    2.5.References
CHAPTER3.TETRACYCLINE STRIPS BASED ON IRON(III)AND COPPER(II)IMPREGNATED IMMOBILIZED NANOFIBROUS MEMBRANES
    3.1.Introduction
    3.2.Experimental
        3.2.1.Chemicals and materials
        3.2.2.Preparation of Fe@alginate/PAN nanofibers
        3.2.3.Preparation of Cu@EPAN nanofibers
        3.2.4.Characterization of TCs sensing performance
        3.2.5.Characterization
    3.3.Results and discussion
        3.3.1.Fe@alginate/PAN nanofibers
        3.3.2.Cu@EPAN nanofibers
    3.4.Chapter summary
    3.5.References
CHAPTER4.METRONIDAZOLE NON-ENZYMATIC COLORIMETRIC SENSORS STRIP BASED ON MELAMINE-FUNCTIONALIZED GOLD NANOPARTICLES ASSEMBLED POLYAMIDE NANOFIBERS MEMBRANES
    4.1.Introduction
    4.2.Experimental
        4.2.1.Chemicals and materials
        4.2.2.Preparation and functionalization of gold nanoparticles
        4.2.3.Preparation of the PA6 NFMs
        4.2.4.Preparation of colorimetric strips and sensing experiment
        4.2.5.Real samples preparation
        4.2.6.Characterization
    4.3.Results and discussion
        4.3.1.Sensing concept
        4.3.2.Characteristics of AuNPs and MA@AuNPs
        4.3.3.Characterization of MA@AuNPs immobilized PA6 NFMs
        4.3.4.Optimization of detection conditions
        4.3.5.The detection performance of strips
    4.4.Chapter summary
    4.5.References
CHAPTER5.KANAMYCIN COLORIMETRIC STRIPS BASED ON APTAMER-GOLD NANOPARTICLE IMMOBILIZED NANOFIBROUS MEMBRANES
    5.1.Introduction
    5.2.Experimental
        5.2.1.Chemicals and materials
        5.2.2.Preparation and bioconjugation of gold nanoparticles
        5.2.3.G-CA NFMs fabrication
        5.2.4.Apt@Au assembled G-CA NFMs and experimented for KMC detection
        5.2.5.Real samples preparation
        5.2.6.Characterization
    5.3.Results and discussion
        5.3.1.Design and principle of the proposed biosensing
        5.3.2.Apt@Au probes characterization
        5.3.3.Characterization of GA grafted CA NFMs
        5.3.4.Characterization of Apt@Au assembled GA grafted CA NFMs
        5.3.5.Biosensing performance analysis
    5.4.Chapter summary
    5.5.References
CHAPTER6.CONCLUSIONS AND RECOMMENDATIONS
    6.1.Conclusions
    6.2.Future work and recommendations
LIST OF PUBLICATIONS
APPENDIX A



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