本文選題：藍光光盤/光驅(qū) + 現(xiàn)場快速檢測�。� 參考：《太原理工大學(xué)》2017年碩士論文

【摘要】：藍光光盤(Blu-ray Disc,BD)不僅可以作為全高清格式電影和大型電子游戲的存儲媒介,并且憑借其優(yōu)良的光學(xué)性能、納米級的表面平整度,使其還可以作為化學(xué)分析和生物分子檢測反應(yīng)的基底材料,同時結(jié)合藍光光驅(qū)精密的伺服控制系統(tǒng)和光學(xué)讀取機制,藍光光盤/光驅(qū)有望成為新一代的高通量、高靈敏度的現(xiàn)場快速檢測(point-of-care testing,POCT)工具。本文以藍光光驅(qū)讀寫原理和光盤存儲技術(shù)為基礎(chǔ),通過提取、采集標準藍光光驅(qū)內(nèi)部光學(xué)頭的光強信號,在Windows環(huán)境下研發(fā)了基于標準藍光光盤/光驅(qū)的成像檢測系統(tǒng)。通過測試打印高通量陣列BD光盤,驗證了該系統(tǒng)的可行性、分辨率(至少可以達到100μm)以及檢測通量(至少可以實現(xiàn)1728個待測樣品同時檢測),同時成功制備了黃曲霉毒素B1(Aflatoxin B1,AFB1)免疫優(yōu)化反應(yīng)BD光盤,并利用所研發(fā)的成像系統(tǒng)實現(xiàn)了AFB1抗體濃度的優(yōu)化反應(yīng)實驗的定量檢測�；跇藴仕{光光盤/光驅(qū)的成像檢測系統(tǒng)可以作為一個完整的檢測平臺應(yīng)用于食品安全、水質(zhì)監(jiān)測和醫(yī)療診斷等重要領(lǐng)域。
[Abstract]:Blu-ray Disct BDB can be used not only as a storage medium for full-HD movies and large video games, but also for its excellent optical properties and nanometer surface smoothness. It can also be used as a substrate material for chemical analysis and biomolecular detection reactions. At the same time, combined with the precise servo control system and optical reading mechanism of Blu-ray optical drive, Blu-ray optical disc / optical drive is expected to become a new generation of high-throughput. High sensitivity on-the-spot rapid detection point-of-care testing POCTs. Based on the principle of reading and writing of Blu-ray optical drive and optical disk storage technology, this paper develops an imaging detection system based on standard Blu-ray optical disk / optical drive in Windows environment by extracting and collecting the light intensity signal of the optical head inside the standard Blu-ray optical drive. The feasibility of the system is verified by testing and printing high throughput array BD optical disk. At least 100 渭 m resolution (at least 100 渭 m) and detection flux (at least 1728 samples can be detected simultaneously, and aflatoxin B1(Aflatoxin B1 AFB1) immuno-optimization reaction optical disk (BD) was successfully prepared. The optimized reaction test of AFB1 antibody concentration was carried out by using the imaging system developed in this paper. The imaging detection system based on standard Blu-ray disc / optical drive can be used as a complete detection platform in important fields such as food safety, water quality monitoring and medical diagnosis.
【學(xué)位授予單位】：太原理工大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：TP333.4

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