赤霉病和條銹病對(duì)小麥產(chǎn)量的影響及小麥L693 2B染色體上抗赤霉病QTL定位
發(fā)布時(shí)間:2023-04-04 05:38
小麥赤霉病主要是由禾谷鐮刀菌(鐮孢赤霉菌)引起的穗部病害,而小麥條銹病是由小麥條銹菌引起的葉部病害。他們各自對(duì)小麥產(chǎn)量的影響已經(jīng)很清楚,但是小麥赤霉病和小麥條銹病互作效應(yīng)對(duì)產(chǎn)量的影響還鮮有報(bào)道。在本研究中,利用來自于L661/L693雜交組合的F2群體作為研究主體材料,并且利用組成分分析方法來研究小麥赤霉病和小麥條銹病互作對(duì)穗粒數(shù)、穗粒重、千粒重的影響。結(jié)果表明:與條銹病相比較,赤霉病對(duì)千粒重有較大的影響,而對(duì)穗粒數(shù)影響較小,并且赤霉病和條銹病各自對(duì)所有性狀的影響都達(dá)到了極顯著水平,但是赤霉病和條銹病互作的影響比單獨(dú)的影響更加顯著。另外,對(duì)小麥赤霉病和小麥條銹病對(duì)小麥產(chǎn)量的影響的比較結(jié)果可以看出過去人們低估了小麥赤霉病對(duì)小麥產(chǎn)量的影響,可能是因?yàn)榭茖W(xué)家們對(duì)赤霉病更多的關(guān)注點(diǎn)在赤霉病對(duì)小麥品質(zhì)而不是小麥產(chǎn)量。L693/L661雜交產(chǎn)生的RIL群體329份,100多個(gè)SSR標(biāo)記用于遺傳圖譜構(gòu)建。該遺傳圖譜總長(zhǎng)度112 cM,平均距離為8 cM。通過QTL定位分析,發(fā)現(xiàn)4個(gè)不病小穗數(shù)相關(guān)QTL,這些QTL可以用于赤霉病抗性聚合育種。
【文章頁數(shù)】:87 頁
【學(xué)位級(jí)別】:博士
【文章目錄】:
abstract
摘要
List of Abbreviations
CHAPTER 1–Literature Review
1.1.The evolution of cultivated wheat
1.2.Wheat genetic system
1.2.1.Genetic stocks
1.2.2.Cytogenetic and molecular mapping
1.2.3.Comparative mapping
1.2.4.Positional cloning
1.3.Wheat production and utilization
1.3.1.Wheat production in china
1.4.Fusarium head blight of wheat
1.4.1.Pathogens
1.4.2.Natural FHB infection and development
1.4.3.Economic loss from FHB
1.4.4.Strategies for control of Fusarium head blight
1.5.Wheat resistance to Fusarium head blight
1.5.1.Types of resistance
1.5.2.Mechanisms of resistance
1.5.3.Sources of resistance
1.5.4.Inheritance of resistance
1.5.5.Durability of resistance
1.6.Molecular markers
1.6.1.RFLP markers
1.6.2.PCR-based markers
1.7.QTL for FHB resistance
1.7.1.Mapping FHB resistance QTL in wheat
1.8.Breeding for FHB resistance in wheat
1.8.1.Conventional breeding strategies
1.8.2.Marker-assisted breeding
1.9.Yellow Rust
1.9.1.Pathogen Over-summering
1.9.2.Background of Stripe Rust Resistance in Wheat
1.9.3.Simulation of Virulence Variation and Prediction of Resistance Durability
1.9.4.Cytology of interaction between the host and pathogen
1.9.5.Development of Near Isogenic Lines(NILs)
1.9.6.Identification of Molecular Markers for Resistance Genes
1.9.7.Other Studies
1.10.Management of Wheat Stripe Rust
1.10.1.Resistance Breeding
1.10.2.Resistance Deployment
CHAPTER 2-Effects of Fusarium Head Blight and Yellow rust on wheat with relevance to source sink relationship.
2.1.Introduction
2.2.Material and Method
2.2.1.Plant material and experimental design
2.2.2.Resistance Evaluation
2.2.3.Statistical Analysis
2.2.4.Principle Component Analysis(PCA)
2.3.Results
2.4.Discussion
CHAPTER 3-QTL for three types of resistance to Fusarium head blight in a wheat population from L661/L
3.1.Introduction
3.2.Materials and Method
3.2.1.Plant Material and Pathogen
3.2.2.Evaluation of FHB
3.2.3.Measurement of Yield Components
3.2.4.Statistical Analysis
3.2.5.SSR Assay
3.2.6.Experimental Desisgn and Data Analysis
3.3.Results
3.3.1.FHB Infection and Resistance
3.3.2.Linkage Molecular Marker Screening
3.3.3.Mapping of Major QTLs
3.4.Discussion
3.4.1.Mode of Inheritance for FHB Resistance
3.4.2.Chromosomal location and Genetic Mapping of FHB Resistance QTLs
3.4.3.Origin of FHB Resistance and the putative mechanism of FHB Resistance Transfer
3.4.4.Potential utilization of the FHB resistance QTLs in wheat breeding programs
Referances
Acknowledgements
本文編號(hào):3781887
【文章頁數(shù)】:87 頁
【學(xué)位級(jí)別】:博士
【文章目錄】:
abstract
摘要
List of Abbreviations
CHAPTER 1–Literature Review
1.1.The evolution of cultivated wheat
1.2.Wheat genetic system
1.2.1.Genetic stocks
1.2.2.Cytogenetic and molecular mapping
1.2.3.Comparative mapping
1.2.4.Positional cloning
1.3.Wheat production and utilization
1.3.1.Wheat production in china
1.4.Fusarium head blight of wheat
1.4.1.Pathogens
1.4.2.Natural FHB infection and development
1.4.3.Economic loss from FHB
1.4.4.Strategies for control of Fusarium head blight
1.5.Wheat resistance to Fusarium head blight
1.5.1.Types of resistance
1.5.2.Mechanisms of resistance
1.5.3.Sources of resistance
1.5.4.Inheritance of resistance
1.5.5.Durability of resistance
1.6.Molecular markers
1.6.1.RFLP markers
1.6.2.PCR-based markers
1.7.QTL for FHB resistance
1.7.1.Mapping FHB resistance QTL in wheat
1.8.Breeding for FHB resistance in wheat
1.8.1.Conventional breeding strategies
1.8.2.Marker-assisted breeding
1.9.Yellow Rust
1.9.1.Pathogen Over-summering
1.9.2.Background of Stripe Rust Resistance in Wheat
1.9.3.Simulation of Virulence Variation and Prediction of Resistance Durability
1.9.4.Cytology of interaction between the host and pathogen
1.9.5.Development of Near Isogenic Lines(NILs)
1.9.6.Identification of Molecular Markers for Resistance Genes
1.9.7.Other Studies
1.10.Management of Wheat Stripe Rust
1.10.1.Resistance Breeding
1.10.2.Resistance Deployment
CHAPTER 2-Effects of Fusarium Head Blight and Yellow rust on wheat with relevance to source sink relationship.
2.1.Introduction
2.2.Material and Method
2.2.1.Plant material and experimental design
2.2.2.Resistance Evaluation
2.2.3.Statistical Analysis
2.2.4.Principle Component Analysis(PCA)
2.3.Results
2.4.Discussion
CHAPTER 3-QTL for three types of resistance to Fusarium head blight in a wheat population from L661/L
3.1.Introduction
3.2.Materials and Method
3.2.1.Plant Material and Pathogen
3.2.2.Evaluation of FHB
3.2.3.Measurement of Yield Components
3.2.4.Statistical Analysis
3.2.5.SSR Assay
3.2.6.Experimental Desisgn and Data Analysis
3.3.Results
3.3.1.FHB Infection and Resistance
3.3.2.Linkage Molecular Marker Screening
3.3.3.Mapping of Major QTLs
3.4.Discussion
3.4.1.Mode of Inheritance for FHB Resistance
3.4.2.Chromosomal location and Genetic Mapping of FHB Resistance QTLs
3.4.3.Origin of FHB Resistance and the putative mechanism of FHB Resistance Transfer
3.4.4.Potential utilization of the FHB resistance QTLs in wheat breeding programs
Referances
Acknowledgements
本文編號(hào):3781887
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