大豆疫霉RxLR效應(yīng)分子Avh52的功能與作用機(jī)制研究
發(fā)布時(shí)間:2021-11-26 18:17
大豆疫霉(Phytophthora sojae)引起的大豆疫霉根腐病,是大豆生產(chǎn)中的一種毀滅性病害,平均每年給全世界大豆生產(chǎn)造成10-20億美元的經(jīng)濟(jì)損失。大豆疫霉在侵染大豆的過(guò)程中會(huì)分泌大約400個(gè)RxLR(Arg-any amino acid-Leu-Arg)效應(yīng)分子,這些效應(yīng)分子在病原菌侵染寄主的過(guò)程中發(fā)揮著重要的作用。有些效應(yīng)分子能夠誘發(fā)植物產(chǎn)生免疫反應(yīng),有些效應(yīng)分子則會(huì)抑制植物產(chǎn)生的免疫反應(yīng),這些效應(yīng)分子程序性表達(dá)、協(xié)同作用幫助病原菌致病。基于本實(shí)驗(yàn)室前期的研究發(fā)現(xiàn)大豆疫霉菌分泌的RxLR效應(yīng)分子PsAvh52能夠抑制PMAPs(INF1和XEG1)和效應(yīng)分子(PsAvh238和PsAvh241)引起的細(xì)胞壞死。本研究在此基礎(chǔ)上對(duì)PsAvh52的毒性功能作了進(jìn)一步的探索,鑒定到了其在寄主中的靶標(biāo)蛋白GmTAP1,揭示了 PsAvh52促進(jìn)大豆疫霉侵染的分子機(jī)制。大豆疫霉RxLR效應(yīng)分子PsAvh52毒性功能分析。本實(shí)驗(yàn)研究發(fā)現(xiàn)在大豆疫霉侵染前期,分泌的一個(gè)高表達(dá)的RxLR效應(yīng)分子PsAvh52,是大豆疫霉全毒力必需的效應(yīng)分子。PsAvh52在四個(gè)代表大豆疫霉生理小種的菌株間存...
【文章來(lái)源】:南京農(nóng)業(yè)大學(xué)江蘇省 211工程院校 教育部直屬院校
【文章頁(yè)數(shù)】:143 頁(yè)
【學(xué)位級(jí)別】:博士
【部分圖文】:
圖1-2植物免疫系統(tǒng)模式圖??Figure?1-2?Schematic?of?the?plant?immune?system.?(Dangl?et?al.?2013)??
4?2!?%??細(xì)內(nèi)*—?OCN5^AOA2?g?.??Svkx^sars0?mA21?<5??G£NSi;|?C??PSR,?一^?V?訪(fǎng)??Cytoplasm??、、'?、?…"一:―一^一一:-?.??'■????,?.......??.?,..??X?■?■?.■-:...?■.?.?■?-??:_?■….‘■:■?■???..-V.?-??Cifmsie?Opr^t?妁??圖1-3大豆疫霉效應(yīng)分子及其靶標(biāo)互作模型??Figure?1-3?Working?models?of?several?characterized?P.?sojae?effectors?and?their?corresponding?host??targets?summarized?based?on?the?current?knowledge?(wang?et?al.?2018)??P.?sojae?secretes?effectors?that?act?in?different?cellular?compartments.?In?the?apoplast,?XEG1?plays?dual??roles?in?plant-pathogen?interactions.?XEG1?acts?as?a?virulence?effector?under?the?protection?of?its?close??ortholog?XLP1?which?competes?for?GIP1?binding?site?to?counter?the?inhibition?of
\Acy?methyltransferase?-?CM??|?t。力.??Nuc,eos〇m.?,?&C??Chromosom.?,&???methylated?cytosine?base?H?Transcription????unmethylated?cytosine?base?/??^?histone?tail?/??Chromatin?^??/?為,?????s?Messenger?RNA?(mRNA)??microRNA?(miRNA)?^??圖2-1表觀(guān)遺傳修飾的類(lèi)型??Figure?2-1?Types?of?epigenetic?modifications?(Zhou?et?al.,2010)??(A)?Histones?can?undergo?phosphorylation?(Ph),?meth>?lation?(Me),?and?acetylation?(Ac),?among?other?chemical??modifications.?These?modifications?are?involved?in?chromatin?remodeling?and?transcriptionaJ?regulation.?(B)?DNA??molecules?are?methylated?by?the?addition?of?a?methyl?group?to?carbon?position?5?on?cjlosine?bases,?a?raiction?catalyzed?by??DNA?methyllransferase?enzymes,which?maintains?repressed?ge
【參考文獻(xiàn)】:
期刊論文
[1]Induced Pib Expression and Resistance to Magnaporthe grisea are Compromised by Cytosine Demethylation at Critical Promoter Regions in Rice[J]. Yuan Li1?,Qiong Xia1?,Hongping Kou2,Dan Wang2,Xiuyun Lin3,Ying Wu1,Chunming Xu1,Shaochen Xing3 and Bao Liu1 1Key Laboratory of Molecular Epigenetics of MOE and The Institute of Genetics & Cytology,Northeast Normal University,Changchun 130024,China 2Department of Agronomy,Jilin Agricultural University,Changchun 130118,China 3Center of Agribiotechnology,Jilin Academy of Agricultural Sciences,Changchun 130033,China. Journal of Integrative Plant Biology. 2011(10)
本文編號(hào):3520680
【文章來(lái)源】:南京農(nóng)業(yè)大學(xué)江蘇省 211工程院校 教育部直屬院校
【文章頁(yè)數(shù)】:143 頁(yè)
【學(xué)位級(jí)別】:博士
【部分圖文】:
圖1-2植物免疫系統(tǒng)模式圖??Figure?1-2?Schematic?of?the?plant?immune?system.?(Dangl?et?al.?2013)??
4?2!?%??細(xì)內(nèi)*—?OCN5^AOA2?g?.??Svkx^sars0?mA21?<5??G£NSi;|?C??PSR,?一^?V?訪(fǎng)??Cytoplasm??、、'?、?…"一:―一^一一:-?.??'■????,?.......??.?,..??X?■?■?.■-:...?■.?.?■?-??:_?■….‘■:■?■???..-V.?-??Cifmsie?Opr^t?妁??圖1-3大豆疫霉效應(yīng)分子及其靶標(biāo)互作模型??Figure?1-3?Working?models?of?several?characterized?P.?sojae?effectors?and?their?corresponding?host??targets?summarized?based?on?the?current?knowledge?(wang?et?al.?2018)??P.?sojae?secretes?effectors?that?act?in?different?cellular?compartments.?In?the?apoplast,?XEG1?plays?dual??roles?in?plant-pathogen?interactions.?XEG1?acts?as?a?virulence?effector?under?the?protection?of?its?close??ortholog?XLP1?which?competes?for?GIP1?binding?site?to?counter?the?inhibition?of
\Acy?methyltransferase?-?CM??|?t。力.??Nuc,eos〇m.?,?&C??Chromosom.?,&???methylated?cytosine?base?H?Transcription????unmethylated?cytosine?base?/??^?histone?tail?/??Chromatin?^??/?為,?????s?Messenger?RNA?(mRNA)??microRNA?(miRNA)?^??圖2-1表觀(guān)遺傳修飾的類(lèi)型??Figure?2-1?Types?of?epigenetic?modifications?(Zhou?et?al.,2010)??(A)?Histones?can?undergo?phosphorylation?(Ph),?meth>?lation?(Me),?and?acetylation?(Ac),?among?other?chemical??modifications.?These?modifications?are?involved?in?chromatin?remodeling?and?transcriptionaJ?regulation.?(B)?DNA??molecules?are?methylated?by?the?addition?of?a?methyl?group?to?carbon?position?5?on?cjlosine?bases,?a?raiction?catalyzed?by??DNA?methyllransferase?enzymes,which?maintains?repressed?ge
【參考文獻(xiàn)】:
期刊論文
[1]Induced Pib Expression and Resistance to Magnaporthe grisea are Compromised by Cytosine Demethylation at Critical Promoter Regions in Rice[J]. Yuan Li1?,Qiong Xia1?,Hongping Kou2,Dan Wang2,Xiuyun Lin3,Ying Wu1,Chunming Xu1,Shaochen Xing3 and Bao Liu1 1Key Laboratory of Molecular Epigenetics of MOE and The Institute of Genetics & Cytology,Northeast Normal University,Changchun 130024,China 2Department of Agronomy,Jilin Agricultural University,Changchun 130118,China 3Center of Agribiotechnology,Jilin Academy of Agricultural Sciences,Changchun 130033,China. Journal of Integrative Plant Biology. 2011(10)
本文編號(hào):3520680
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