發(fā)布時(shí)間：2023-01-07 09:28

　　目的研究脂多糖（LPS）刺激能否誘導(dǎo)正常人肝內(nèi)膽管上皮細(xì)胞（HIBECs）白細(xì)胞介素-6（IL-6）/信號(hào)轉(zhuǎn)導(dǎo)與轉(zhuǎn)錄激活因子3（STAT3）信號(hào)通路的激活及對(duì)該信號(hào)通路下游因子c-Myc和髓樣細(xì)胞白血病-1（Mcl-1）表達(dá)的影響;探討LPS對(duì)HIBECs細(xì)胞增殖的影響及其可能存在的機(jī)制。方法（1）HIBECs細(xì)胞常規(guī)體外培養(yǎng),使用不同濃度的LPS（0、0.1、1、2、4、8μg/ml）分別刺激該細(xì)胞,并分別作用24h、48h、72h,酶聯(lián)免疫吸附實(shí)驗(yàn)（ELISA）檢測(cè)各濃度、各時(shí)間點(diǎn)IL-6的表達(dá)水平,確定LPS的最適刺激濃度及最佳處理時(shí)間,再以其進(jìn)行后續(xù)實(shí)驗(yàn);（2）用前述所測(cè)LPS刺激HIBECs細(xì)胞,并設(shè)立正常細(xì)胞對(duì)照組,Western blot檢測(cè)磷酸化的STAT3（p-STAT3）蛋白和STAT3蛋白表達(dá)情況,確定pSTAT3/STAT3蛋白比例,探討LPS能否激活I(lǐng)L-6/STAT3信號(hào)通路;（3）再以前述所測(cè)LPS干預(yù)HIBECs細(xì)胞,同時(shí)設(shè)立正常細(xì)胞對(duì)照組,分別采用熒光定量逆轉(zhuǎn)錄PCR（RT-q PCR）和Western blot測(cè)定c-Myc、Mcl-1的m RNA及... 

【文章頁(yè)數(shù)】：50 頁(yè)

【學(xué)位級(jí)別】：碩士

【文章目錄】：
英文縮略詞表
中文摘要
英文摘要
1.前言
2.材料與方法
3.結(jié)果
4.討論
5.結(jié)論
參考文獻(xiàn)
附錄
致謝
綜述
    參考文獻(xiàn)


【參考文獻(xiàn)】：
期刊論文
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