富硒地龍對大鼠骨折BMP-2、VEGF表達的影響
[Abstract]:Aim: to observe the effect of selenium-enriched Dilong on the expression of bone morphogenetic protein-2 and vascular endothelial growth factor (VEGF) in the process of left femoral fracture healing in rats, and to explore the promoting effect of selenium-enriched Dilong on fracture healing and its possible mechanism. Methods: 72 male SD rats were selected to make the model of left thigh shaft fracture. After the successful replication of the model, the model was divided into three groups: injured Jiegu tablet group, selenium-enriched Dragon Group group and saline group with 24 rats in each group. After operation, selenium-enriched Dilong 300 mg/ (kg.d)-1, Shangke Jiegu tablet 300 mg/ (kg.d)-1, saline group were given the same volume of saline intragastrically. At 2, 4 and 6 weeks after operation, 8 rats in each group were taken to observe the gross specimens of femur, the volume of bone eschar was measured, DR film and HE staining, serum biochemical detection of BMP-2 expression was detected by VEGF, and the results were statistically analyzed. Result: 1. Imaging findings of fracture end: at the 2nd week after operation, a small amount of callus could be seen in each group, and clear broken bone lines could be seen in all groups, and in the 4th week, most of the specimens in each group had formed bone eschar, and the fracture line was blurred with the continuity of callus tissue passing through the broken end of fracture. At the 6th week, the bone healing standard was reached in all groups, and the fracture line disappeared. According to the score statistics, there was no significant difference among the groups in each period (P 0.05). 2. Gross specimen observation and eschar measurement: at the 2nd week after operation, the callus formation was obvious in each group, the volume was large and the texture was slightly soft, and the volume and texture of each group became smaller and harder at the 4th week. At the 6th week, the volume of eschar was further reduced, the eschar was rebuilt and molded, and there was no difference between the two groups. The volume of eschar was measured. At the 4th week, there was significant difference between the selenium-enriched Dragon Group group and the injured bone tablet group and saline group (P 0.05). Histologic observation of eschar: at the 2nd week, the formation of bone trabeculae could be seen in the broken bone and under the outer membrane of each group, and there was a fibrous tissue connection between the broken ends of the fracture. The amount of bone formation in the membrane and the area of cartilage island in the selenium-enriched Dragon Group group and the injured group were significantly larger than those in the saline group. Week 4: the bone trabeculae of each group were further thickened, inserted and arranged, and connected to the broken end of the fracture. At the 6th week, the bone trabeculae between the fracture ends of the injured bone group and the selenium-enriched Dragon Group fracture were completely transformed into lamellar bone, and the pulp cavity was recanalized. In saline group, the bone trabeculae gradually transitioned to lamellar bone, and some of the specimens were recanalized. Statistical analysis of selenium-enriched Dragon Group and saline group was statistically significant (P 0.05). 4. The expression of BMP-2 was positive in fibroblasts, chondrocytes and osteoblasts in eschar tissues of each group, and mainly in osteoblasts, osteocytes in trabecular meshwork and immature cartilage cells near scab in the early stage of the expression of bFGF in osteoblasts, osteoblasts and osteoblasts in the cytoplasm of osteoblasts, osteoblasts and osteoblasts in the cytoplasm of osteoblasts, osteoblasts and osteoblasts in each group. Osteoblasts were mainly expressed in the later stage, and the depth of cytoplasmic staining of selenium-enriched Dragon Group in Jiegu tablet group and selenium-enriched group was significantly higher than that in saline group. The MOD value of BMP-2 positive expression in each group was the highest at the 2nd week, began to decrease slowly at the 4th week, decreased most significantly at the 6th week, and the positive expression was the lowest. After statistical treatment, the second week: there was significant difference between selenium-enriched Dragon Group group and Shangke Jiegu tablet group and saline group (P 0.05), 4 and 6 weeks, and the difference between the two groups was statistically significant (P 0.05), and there was significant difference between the two groups (P 0.05). There was significant difference between selenium-enriched Dragon Group and Shangke Jiegu tablet group and saline group (P 0.05). The expression of VEGF in peripheral serum of saline group was similar to that of normal group. The expression of VEGF in peripheral serum of selenium-enriched Dragon Group was significantly higher than that of saline group (P 0.05), and the expression of VEGF in peripheral serum of Shangke Jiegu tablet group was higher than that of model group (P 0.05). Conclusion: selenium-enriched Dilong solution can promote the expression of BMP-2 in eschar and VEGF in serum, which may be the mechanism of fracture healing.
【學位授予單位】:湖北民族學院
【學位級別】:碩士
【學位授予年份】:2017
【分類號】:R285.5
【參考文獻】
相關期刊論文 前10條
1 寧濤;胡勇;;復方續(xù)斷三七提取物對家兔骨折愈合的促進作用及其機制[J];西安交通大學學報(醫(yī)學版);2016年06期
2 蘇小強;;自擬益氣補血方輔助股骨近端髓內釘內固定術治療高齡股骨粗隆間骨折療效觀察[J];現(xiàn)代中西醫(yī)結合雜志;2016年28期
3 張玉鈞;;中藥熏洗輔助治療骨折病患者的臨床療效[J];醫(yī)療裝備;2016年18期
4 彭印文;陳棉智;張志輝;;骨后康顆粒對老年股骨粗隆間骨折PFNA術后的療效觀察[J];中成藥;2016年08期
5 柯美珍;;退癀散外敷治療閉合性骨折早期50例[J];光明中醫(yī);2016年15期
6 閔重函;周瑛;張洪美;陳誠;;手法復位、克氏針固定輔以單味桂枝煎湯內服治療小兒肱骨遠端Gartland Ⅲ型骨折35例臨床觀察[J];中醫(yī)兒科雜志;2016年04期
7 林曉陽;張佳榕;徐朝和;;老年橈骨遠端骨折結合補腎活血湯治療的效果分析[J];中外醫(yī)療;2016年21期
8 陶巍;;骨碎補治療成人踝骨骨折的藥理研究[J];中國醫(yī)院用藥評價與分析;2016年S1期
9 趙立偉;;膏藥外貼配合中藥熏洗治療鎖骨骨折的臨床應用療效分析[J];中西醫(yī)結合心血管病電子雜志;2016年15期
10 章黎;高建莉;張樺;李召東;;傷科接骨片對MC3T3-E1細胞成骨能力的影響[J];浙江中西醫(yī)結合雜志;2016年05期
相關博士學位論文 前2條
1 畢鍇;中醫(yī)骨折分期治療對骨折愈合bFGF、TGF-β、VEGF、BMP-2基因表達影響的實驗研究[D];中國中醫(yī)科學院;2013年
2 徐穎鵬;骨折三期治則對家兔骨折愈合及血管內皮生長因子基因表達的影響[D];中國中醫(yī)科學院;2007年
相關碩士學位論文 前9條
1 李暈;復方獨正桿接骨膏外敷對家兔骨折愈合過程中BMP-9、VEGF及Smad-6基因表達機制的研究[D];湖北民族學院;2015年
2 單清潔;辨證使用骨蝕寧膠囊Ⅰ號、Ⅱ號對激素性股骨頭壞死家兔模型VEGF表達的影響及組織學觀察[D];安徽中醫(yī)藥大學;2015年
3 肖鋒;去瘀生新外治法及接骨膏對家兔骨折愈合中BMP-2表達的實驗研究[D];湖北民族學院;2014年
4 王冠;骨傷復原丸配合外固定支架治療脛腓骨骨折的臨床觀察[D];黑龍江中醫(yī)藥大學;2014年
5 佟亮;復方接骨片促進骨折愈合的臨床研究[D];遼寧中醫(yī)藥大學;2012年
6 郭威;丹參酮ⅡA對大鼠骨髓間充質干細胞成骨分化影響的實驗研究[D];遼寧醫(yī)學院;2012年
7 李翠芬;模擬自然環(huán)境生長的斷體地龍促進創(chuàng)傷愈合成分的分離及篩選[D];北京中醫(yī)藥大學;2011年
8 張秀杰;復元活血湯對SD大鼠骨折早期血管內皮生長因子表達影響的實驗研究[D];山東中醫(yī)藥大學;2010年
9 湯耿民;補腎活血法促進骨折愈合的機理研究[D];福建中醫(yī)學院;2000年
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