本文關(guān)鍵詞：殺傷性人工抗原提呈細(xì)胞治療小鼠皮膚移植排斥及其對治療鼠整體免疫功能的影響，由筆耕文化傳播整理發(fā)布。

殺傷性人工抗原提呈細(xì)胞治療小鼠皮膚移植排斥及其對治療鼠整體免疫功能的影響

Killer artificial antigen-presenting cells treating skin allograft rejection and its impact on the overall immune function in mice model

[1] [2] [3] [4] [5] [6] [7]

LI Miao-chen,WANG Wei,LI Xiao-e,FANG Kun,WU You,GU Ning,SHEN Chuan-lai （ 1. Department of Pathogenic Biology and Immunology, School of Medicine, Southeast University, Nanjing 210

[1]東南大學(xué)醫(yī)學(xué)院病原生物學(xué)與免疫學(xué)系,江蘇南京210009; [2]東南大學(xué)生物科學(xué)與醫(yī)學(xué)工程學(xué)院,江蘇南京210009

文章摘要：目的：研究殺傷性人工抗原提呈細(xì)胞（Ka APC）治療小鼠皮膚移植排斥的效果及其對治療鼠整體免疫功能的影響。方法：利用可生物降解的聚乳酸-羥基乙酸共聚物微米球做載體,共價吸附H-2Kb抗原和anti-Fas等負(fù)性調(diào)節(jié)分子,制備針對同種反應(yīng)性T細(xì)胞的特異性Ka APC,治療以C57BL/6和BALB/c為供受對的小鼠皮膚移植排斥,并利用混合淋巴細(xì)胞培養(yǎng)觀察治療鼠對第三方供體的同種反應(yīng)能力以及通過載瘤實驗觀察治療鼠的抗腫瘤能力等,以論證Ka APC治療對機(jī)體整體免疫功能的影響,幫助篩選有效治療方案。結(jié)果：成功制備Ka APC,FACS驗證其有正確的表型;Ka APC輸注使皮膚移植物的存活時間明顯延長,P〈0.000 1,但并未顯著損傷治療鼠T細(xì)胞對第三方供體鼠脾細(xì)胞的同種增殖能力,也并未顯著損傷治療鼠的抗腫瘤能力。結(jié)論：Ka APC可以抑制小鼠皮膚移植排斥并對治療鼠的整體免疫功能無明顯損傷。

Abstr：Objective： To study the therapeutic effect for skin allograft rejection of killer artificial antigenpresenting cells（ Ka APC） and its impact on the overall immune function in mice model. Methods： Ka APC were prepared by co-coupling H-2Kbantigen and anti-Fas monoclonal antibody onto the biodegradable polylactic-coglycolic acid（ PLGA） microspheres,and then were administered intravenously into BALB / c mice（ H-2Kd） that had previously been grafted with skin squares from C57 BL /6 mice（ H-2Kb）. Tumor cell challenge and third-party mixed lymphocyte culture were used to assess the general immune functions of host. Results： Ka APC microspheres had the correct phenotype as verified by FACS; the skinallograft survival was prolonged significantly（ P〈0. 000 1）after treatment with Ka APC; the proliferation of recipient T cells against the third-party splenocytes and the defense to tumor did not apparently decreased after treatment with Ka APC in therapy mice. Conclusion： The Ka APC have ability to inhibit skin allograft rejection in mice model,without impairment of host overall immune function.

文章關(guān)鍵詞：

Keyword：:killer artificial antigen-presenting cells polylactic-co-glycolic acid alloreactive T cells mice

課題項目：國家自然科學(xué)基金面上項目（81172823;81372448）

  本文關(guān)鍵詞：殺傷性人工抗原提呈細(xì)胞治療小鼠皮膚移植排斥及其對治療鼠整體免疫功能的影響，，由筆耕文化傳播整理發(fā)布。