本文選題：汗腺再生 + 骨髓間充質(zhì)干細(xì)胞(MSC)�。� 參考：《浙江大學(xué)》2017年碩士論文

【摘要】：目的:在誘導(dǎo)汗腺再生的作用上,pDNA-EDA和pDNA-EGF的協(xié)同作用是否比單一的pDNA-EDA的作用更有效。方法:事先制備的負(fù)載骨髓間充質(zhì)干細(xì)胞(MSC)的基因活性膠原-殼聚糖支架。以加入pDNA-EDA的負(fù)載MSC的膠原一殼聚糖/硅橡膠雙層皮膚支架為對照組(EDA組),以加入pDNA-EGF和pDNA-EDA的負(fù)載MSC的膠原—?dú)ぞ厶?硅橡膠雙層皮膚支架為實(shí)驗(yàn)組(EDA+EGF組)。建立SD大鼠腳掌的全層皮膚損傷模型,將以上兩種支架植入皮膚缺損中。術(shù)后2周、4周、8周分別大體觀察傷口愈合情況,并取再生組織標(biāo)本做切片HE染色分析組織學(xué)觀察,采用免疫組化的方法鑒定再生結(jié)構(gòu)是否為汗腺組織。結(jié)果:術(shù)后2周、4周、8周大體觀察對照組(EDA組)與實(shí)驗(yàn)組(EDA+EGF組)在傷口修復(fù)的進(jìn)度上沒有明顯差別,第8周時(shí)兩組大鼠腳掌皮膚均已均愈合完好,且無明顯疤痕遺留。對2周、4周和8周的組織切片進(jìn)行HE染色分析,發(fā)現(xiàn)在8周時(shí)實(shí)驗(yàn)組(EDA+EGF組)切片中的組織結(jié)構(gòu)與正常類似,可見大量脂肪細(xì)胞,并出現(xiàn)類汗腺結(jié)構(gòu),對照組(EDA組)的切片中大部分細(xì)胞還是成纖維細(xì)胞,也浙江大學(xué)碩士學(xué)位論文 中文摘要發(fā)現(xiàn)類似汗腺的結(jié)構(gòu),但數(shù)量較少,結(jié)構(gòu)較簡單。經(jīng)CK8和CK14鑒定陽性,表明該結(jié)構(gòu)為汗腺。結(jié)論:以膠原一殼聚糖/硅橡膠雙層皮膚支架為負(fù)載容器,pDNA-EDA+pDNA-EGF的協(xié)同作用誘導(dǎo)SD大鼠MSC向汗腺分化的能力比單獨(dú)的pDNA-EDA強(qiáng)。
[Abstract]:Aim: whether the synergistic effect of pDNA-EDA and pDNA-EGF is more effective than that of single pDNA-EDA in inducing sweat gland regeneration. Methods: the gene active collagen-chitosan scaffold of MSCs loaded with bone marrow mesenchymal stem cells (MSCs) was prepared in advance. The collagen-chitosan / silicone rubber bilayer skin scaffold loaded with pDNA-EDA was used as the control group, and the collagen-chitosan / silicone rubber double-layer skin scaffold loaded with MSC with pDNA-EGF and pDNA-EDA was used as the experimental group. The full-thickness skin injury model of SD rats was established and the above two scaffolds were implanted into the skin defect. The wound healing was observed at 2 weeks, 4 weeks and 8 weeks after operation, and the tissue sections of regenerated tissue were analyzed by HE staining. Immunohistochemistry was used to identify whether the regenerated structure was sweat gland tissue. Results: there was no significant difference in the progress of wound repair between the control group and the experimental group at 2 weeks and 4 weeks and 8 weeks after operation. At the 8th week, the paw skin of the two groups had healed well, and there was no obvious scar left over. The histological structure of the tissue sections of the experimental group (EDA EGF group) was similar to that of the control group at the 8th week. A large number of adipocytes and sweat gland like structure were observed in the tissue sections at the 2nd week and 4th week and 8th week, respectively. In the control group (EDA group), most of the cells were still fibroblasts. The structure similar to sweat glands was found in Zhejiang University Master's degree thesis in Chinese, but the number was small and the structure was simple. The structure was identified as sweat gland by CK8 and CK14. Conclusion: the synergistic effect of collagen-chitosan / silicone rubber bilayer skin scaffold pDNA-EDA pDNA-EGF on inducing the differentiation of MSC into sweat glands in SD rats is stronger than that of pDNA-EDA alone.
【學(xué)位授予單位】：浙江大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R334.5
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本文編號：1949056