發(fā)布時(shí)間：2018-05-22 18:56

  本文選題：高尿酸血癥模型 + 化濕降濁　； 參考：《南京中醫(yī)藥大學(xué)》2017年碩士論文

【摘要】：目的:探研導(dǎo)師經(jīng)驗(yàn)方"化濕降濁方"對高尿酸血癥模型大鼠的干預(yù)作用及部分作用機(jī)制,為中藥復(fù)方的臨床應(yīng)用提供一定客觀依據(jù)。方法:采用灌胃次黃嘌呤聯(lián)合氧嗪酸鉀腹腔注射[1]連續(xù)7d以復(fù)制高尿酸血癥模型大鼠。同時(shí)予以別嘌呤醇為陽性藥物對照,研究化濕降濁方(1.2g.ml-1)對各組血尿酸(UA)、肝臟黃嘌呤氧化酶(xanthine oxidase,XOD)、谷丙轉(zhuǎn)氨酶(ALT)、谷草轉(zhuǎn)氨酶(AST)的影響;采用同樣方法7d復(fù)制高尿酸血癥模型大鼠,再予苯溴馬隆為陽性藥物對照,化濕降濁方(1.2g.ml-1)對各組灌胃7d,檢測其對血尿酸(UA)、尿UA、尿素氮(BUN)、肌酐(Scr)的影響,并觀察腎組織切片變化。運(yùn)用SPSS17.0統(tǒng)計(jì)軟件對上述數(shù)據(jù)進(jìn)行分析,以P0.05為差異有統(tǒng)計(jì)學(xué)意義。結(jié)果:1.與空白組對比,模型組大鼠血UA顯著升高(P0.01)。與模型組相比,中藥組能有效抑制模型大鼠肝臟XOD的活性以達(dá)到降低血UA的作用,其差異具有顯著性(P0.01);中藥組較別嘌呤醇組顯著降低ALT、AST含量(P0.05,P0.01)。2.與空白組相比,苯溴馬隆組、中藥組均能有效促進(jìn)模型大鼠尿UA排泄,達(dá)到降低血UA的作用,其差異具有顯著性(P0.01);中藥組較模型組的血清Scr、BUN含量降低(P均0.01);3.腎組織切片方面:化濕降濁組及苯溴馬隆組對模型大鼠腎臟的損害程度均較模型組輕,其中化濕降濁組對模型大鼠腎間質(zhì)損害度較苯溴馬隆組輕,僅見輕度水腫伴少量尿酸結(jié)晶形成。結(jié)論:1.化濕降濁方能有效降低高尿酸血癥模型大鼠的血尿酸水平。2.化濕降濁方能夠有效抑制高尿酸血癥模型大鼠肝臟黃嘌呤氧化酶的活性,并且不會(huì)引起ALT、AST的異常。3.化濕降濁方具有促進(jìn)高尿酸血癥模型大鼠尿酸排泄作用,有一定程度的腎功能保護(hù)作用。
[Abstract]:Objective: to explore the intervention effect and partial mechanism of "Huashi Jiangzhuo prescription" on hyperuricemia model rats, and to provide some objective basis for clinical application of traditional Chinese medicine prescription. Methods: the hyperuricemia model rats were induced by intraperitoneal injection of Hypoxanthine and potassium oxazinate for 7 days. At the same time, allopurinol was given as positive drug control, and the effects of Huazhi Jiangzhuo prescription (1.2g 路ml-1) on serum uric acid, xanthine oxidase XODN, alanine aminotransferase (alt) and aspartate aminotransferase (AST) were studied in rats with hyperuricemia by the same method for 7 days. In addition, benzbromarone was used as the positive drug control, and Huashi Jiangzhuo prescription (1.2g 路ml-1) was given to each group for 7 days, and the effects on serum uric acid, urine UAA, urea nitrogen bun, creatinine Scr1) were detected, and the changes of renal tissue sections were observed. Using SPSS17.0 statistical software to analyze the above data, P0.05 as the difference is statistically significant. The result is 1: 1. Compared with the blank group, the serum UA in the model group was significantly higher than that in the control group (P 0.01). Compared with the model group, the Chinese medicine group could effectively inhibit the activity of XOD in the liver of the model rats to achieve the effect of reducing the blood UA, the difference was significant (P 0.01), and the content of alt in the Chinese medicine group was significantly lower than that in the allopurinol group. Compared with the blank group, the traditional Chinese medicine group could effectively promote the urinary UA excretion of the model rats, and achieve the effect of reducing the blood UA level, the difference was significant (P 0.01), and the serum Scr-bun content in the Chinese medicine group was lower than that in the model group (P < 0.01). In the aspect of renal tissue section, the damage degree of kidney in dehumidification and turbid group and benzbrommalone group was lighter than that in model group, and the degree of renal interstitial damage in dehumidification and turbid group was lighter than that in benzbromine group. Mild edema was observed with a small amount of uric acid crystallization. Conclusion 1. Huashi Jiangzhuo prescription can effectively reduce the blood uric acid level of hyperuricemia rats. 2. 2. Huashi Jiangzhuo prescription can effectively inhibit the activity of xanthine oxidase in liver of hyperuricemia rats and does not cause the abnormality of alt AST. 3. Huashi Jiangzhuo prescription can promote the excretion of uric acid in hyperuricemia rats and protect renal function to some extent.
【學(xué)位授予單位】：南京中醫(yī)藥大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R285.5;R-332

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