本文選題：微等離子體射頻技術 + 增生性瘢痕�。� 參考：《河北醫(yī)科大學》2017年碩士論文

【摘要】：目的:微等離子體射頻技術作為一種治療瘢痕的新技術已在臨床上廣為應用,但對于早期增生性瘢痕的治療研究較少,本實驗通過研究微等離子體射頻技術治療兔耳增生性瘢痕組織后白細胞介素-8(Interleukin 8,IL-8)的表達變化與微血管計數(shù)(Microvessel Count,MVC)的關系,評價微等離子體射頻技術對早期增生性瘢痕的治療效果,為微等離子體射頻技術治療早期增生性瘢痕提供實驗依據(jù)及理論基礎。方法:1選擇6只同屬新西蘭大耳兔,全部為雌兔,體重2.5~3kg。在兔耳腹側面建立增生性瘢痕的模型,造模成功后將每只兔子的雙耳隨機分配到實驗組和對照組,實驗組應用微等離子體射頻技術治療,對照組未予治療。治療30天后切取實驗組和對照組增生性瘢痕組織制作標本,對標本進行固定、脫水、浸石蠟、包埋,切片,切片厚度為4μm。切片采用HE染色及顯微鏡觀察各實驗組與對照組兔耳增生性瘢痕的組織學變化,利用CD34內(nèi)皮細胞抗原標記血管內(nèi)皮細胞顯示瘢痕內(nèi)微血管密度變化,采用免疫組織化學染色檢測瘢痕內(nèi)IL-8表達的變化。2統(tǒng)計各治療組與對照組兔耳增生性瘢痕組織中微血管密度及趨化因子IL-8的平均光密度值,數(shù)據(jù)用均數(shù)±標準差表示。應用SPSS 21.0進行配對設計的t檢驗及相關性分析,以P0.01作為有無顯著性差異的標準。結果:1組織形態(tài)學觀察結果對照組:肉眼可見瘢痕組織突出于皮膚表面,顏色發(fā)紅、質(zhì)地硬;HE染色光鏡下可見真皮層增厚,微血管數(shù)量較多,成纖維細胞及膠原纖維大量增生,排列不規(guī)則。實驗組:肉眼可見瘢痕組織高度較對照組變平,顏色變淡、質(zhì)地變軟;HE染色光鏡下可見真皮層厚度變薄,瘢痕組織內(nèi)微血管的數(shù)量較對照組明顯減少,成纖維細胞數(shù)目減少,膠原纖維束較治療前排列整齊。2免疫組化結果2.1實驗組微血管密度為27.00±5.49個/mm2,對照組微血管密度為48.25±8.51個/mm2,兩組比較差異具有統(tǒng)計學意義(P0.01),表明實驗組微血管密度較對照組微血管密度明顯減少。2.2實驗組增生性瘢痕中IL-8的平均光密度值為0.016±0.011,對照組IL-8的平均光密度值為0.078±0.023,兩組比較差異具有統(tǒng)計學意義(P0.01),表明實驗組IL-8的表達低于對照組。2.3增生性瘢痕中微血管計數(shù)與IL-8的表達呈密切正相關(P0.01)。結論:微等離子體射頻技術對于兔耳增生性瘢痕有一定防治作用;抑制增生性瘢痕組織內(nèi)血管生成及IL-8趨化因子的表達可能是預防和治療增生性瘢痕的新方法。
[Abstract]:Objective: as a new technique for the treatment of scar, microplasma radiofrequency technique has been widely used in clinic, but there is little research on the treatment of early hypertrophic scar. The purpose of this study was to investigate the relationship between the expression of interleukin-8 (IL-8) and microvessel count (MVCC) in rabbit ear hypertrophic scar treated with microplasma radiofrequency (RF) technique, and to evaluate the therapeutic effect of microplasma radiofrequency technique on early hypertrophic scar. To provide experimental and theoretical basis for the treatment of early hypertrophic scar by microplasma radiofrequency technique. Methods six New Zealand big eared rabbits, all of them female, were selected. The hypertrophic scar model was established on the ventral side of the rabbit ear. After the establishment of the model, each rabbit's ears were randomly assigned to the experimental group and the control group. The experimental group was treated with microplasma radiofrequency technique, but the control group was not treated. After 30 days of treatment, the experimental group and the control group were cut out to make specimens of hypertrophic scar. The specimens were fixed, dehydrated, soaked in paraffin, embedded, sliced, and the thickness of sections was 4 渭 m. The histopathological changes of hypertrophic scar were observed by HE staining and microscope in each experimental group and control group. Vascular endothelial cells labeled with CD34 endothelial cell antigen were used to show the change of microvessel density in the scar. Immunohistochemical staining was used to detect the expression of IL-8 in hypertrophic scar. 2 the mean optical density of microvessel density and chemokine IL-8 in hypertrophic scar tissue of rabbit ear were measured by means of mean 鹵standard deviation. T test and correlation analysis of pairing design were carried out with SPSS 21.0, and P0.01 was taken as the criterion of significant difference. Results in the control group, the scar tissue was prominent on the skin surface, the color was red, the dermis was thickened, the number of microvessels was more, and the fibroblasts and collagen fibers were proliferating under light microscope. The arrangement is irregular. In the experimental group, the scar tissue was flattened, the color became lighter, the texture softened, the thickness of the dermis became thinner under HE staining, the number of microvessels in the scar tissue was significantly lower than that in the control group, and the number of fibroblasts was decreased. Collagen bundles were arranged neatly compared with before treatment by immunohistochemistry 2.1 the microvessel density of experimental group was 27.00 鹵5.49 / mm ~ (-2), while that of control group was 48.25 鹵8.51 / mm ~ (2). The difference between the two groups was statistically significant (P 0.01), which indicated that the microvessel density of experimental group was higher than that of control group (P < 0.05). The average optical density of IL-8 in hypertrophic scar was 0.016 鹵0.011 in the control group and 0.078 鹵0.023 in the control group. The difference between the two groups was statistically significant, indicating that the expression of IL-8 in the experimental group was lower than that in the control group. The microvessel count in hypertrophic scar was positively correlated with the expression of IL-8 in control group (P 0.01). Conclusion: microplasma radiofrequency technique can prevent and treat hypertrophic scar in rabbit ear and inhibit angiogenesis and expression of IL-8 chemokine in hypertrophic scar tissue may be a new method to prevent and treat hypertrophic scar.
【學位授予單位】：河北醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R622

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