本文選題：形狀記憶 + 微圖形��； 參考：《西南交通大學(xué)》2017年碩士論文

【摘要】：由于日益增多的心血管疾病患者,對(duì)于小直徑血管支架的需求日益增加。傳統(tǒng)的靜脈支架和人造的聚酯及膨脹聚四氟乙烯血管支架存在著來(lái)源不足,長(zhǎng)期效果差,失敗率高等缺點(diǎn)。基于此,組織工程應(yīng)運(yùn)而生,致力于解決這些問(wèn)題。我們?cè)诒菊撐闹胁捎镁哂行螤钣洃浶阅芎土己蒙锵嗳菪缘?臂的聚乙二醇-聚己內(nèi)酯(6a PEG-PCL)作為支架基底材料,將微圖形轉(zhuǎn)移到支架表面,通過(guò)卷曲的方式制備出表面微圖形變化的多層形狀記憶血管支架。使用正方形和長(zhǎng)方形來(lái)分別調(diào)控內(nèi)皮細(xì)胞和平滑肌細(xì)胞,通過(guò)微圖形的調(diào)控作用再生出與天然動(dòng)脈結(jié)構(gòu)相似的新生血管組織。首先我們通過(guò)涂覆熱壓將模板上的圖形轉(zhuǎn)移到交聯(lián)的6臂聚乙二醇-聚己內(nèi)酯(c-6a PEG-PCL)薄膜上,使一片薄膜上同時(shí)具有正方形和長(zhǎng)方形兩種微圖形。使正方形在內(nèi)側(cè)長(zhǎng)方形在外側(cè),通過(guò)卷曲的方式制備出表面微圖形變化的多層形狀記憶血管支架。在潮濕條件下支架的轉(zhuǎn)變溫度為37℃,并具有良好的形狀記憶性能。同時(shí),支架具有良好的力學(xué)性能和可降解性,體外模擬實(shí)驗(yàn)證明了微創(chuàng)植入的可能,而且支架恢復(fù)后能夠有效地支撐血管。隨后,我們考察了正方形和長(zhǎng)方形對(duì)內(nèi)皮細(xì)胞和平滑肌細(xì)胞行為的影響。支架具有良好的生物相容性和血液相容性,能有效防止血小板的粘附和聚集。正方形可以促進(jìn)內(nèi)皮的增殖和遷移,長(zhǎng)方形能促進(jìn)平滑肌細(xì)胞增殖并誘導(dǎo)其定向的排列。卷曲支架上的共培養(yǎng)發(fā)現(xiàn),正方形能促進(jìn)內(nèi)皮細(xì)胞層粘連蛋白的表達(dá),長(zhǎng)方形能促進(jìn)平滑肌表達(dá)α-平滑肌肌動(dòng)蛋白。最后,我們進(jìn)行了體內(nèi)的支架的血管再生測(cè)試。多普勒超聲發(fā)現(xiàn),植入后血管支架能有效恢復(fù)到永久形狀支撐血管,同時(shí)保持良好的通透性。表面微圖形的血管支架能有效調(diào)控新血管的再生和重構(gòu),正方形能誘導(dǎo)支架內(nèi)層的快速內(nèi)皮化,長(zhǎng)方形調(diào)控平滑肌層環(huán)狀定向排列,并且適宜的炎癥反應(yīng)促進(jìn)新血管組織的再生,支架逐漸降解,最終形成與本體動(dòng)脈結(jié)構(gòu)非常相似的新生動(dòng)脈組織。新生血管具有與本體動(dòng)脈相似的多層結(jié)構(gòu)、細(xì)胞外基質(zhì)含量和爆裂強(qiáng)度。
[Abstract]:As a result of the increasing number of patients with cardiovascular disease, the demand for small diameter stent is increasing. The traditional venous stents, artificial polyester and expanded PTFE vascular stents have some disadvantages, such as insufficient source, poor long-term effect, high failure rate and so on. Based on this, organizational engineering emerged as the times require to solve these problems. In this thesis, we used polyethylene glycol (PEG-PCL), a 6-arm PEG-PCL with shape memory and good biocompatibility, as the substrate material to transfer the micrograph to the surface of the scaffold. A multilayer shape memory vascular stent with surface micrograph changes was fabricated by curling. Square and rectangular cells were used to regulate endothelial cells and smooth muscle cells respectively. The neovascularization tissues similar to the natural artery structure were regenerated by micrographic regulation. First, we transfer the pattern on the template to the cross-linked poly (ethylene glycol) -polycaprolactone (c-6a) PEG-PCL film by hot pressing, and make one thin film have both square and rectangular micrographs. A multi-layer shape memory vascular stent with surface micrograph changes was fabricated by curling the square on the inner rectangle at the outer side. The transition temperature of the scaffold is 37 鈩，

本文編號(hào)：1836020