本文選題：二氧化硅 + 載體分解　； 參考：《南京理工大學(xué)》2017年碩士論文

【摘要】：理想的載藥材料具有在生物體內(nèi)可降解和可控釋放性能,本文以二氧化硅納米材料為藥物載體,制備了載體可分解材料和多條件藥物控釋體系。研究分為兩個部分:(1)介紹了一種酸/堿雙刺激響應(yīng)二氧化硅/抗癌藥物雜化載藥體系(MSNPs-1),由抗癌藥物阿霉素(DOX)和二氧化硅通過改進(jìn)的Stober法制得。用掃描透射電子顯微鏡等測定MSNPs-1結(jié)構(gòu)。用透射電鏡和紫外-可見分光光度計(jì)對其在不同pH條件下藥物釋放情況進(jìn)行研究,證明其在酸性或堿性溶液中可響應(yīng)釋放藥物,同時載體分解。細(xì)胞實(shí)驗(yàn)證明MSNPs-1在7721細(xì)胞中可緩控釋放藥物,為其在治療生物體內(nèi)腫瘤方面的應(yīng)用提供理論基礎(chǔ)。(2)介紹了一種具有pH和紫外光雙響應(yīng)性質(zhì)的智能納米容器(MSNPs-2),其制備方法是在MCM-41表面修飾含有腙鍵結(jié)構(gòu)和偶氮苯衍生物識別位點(diǎn)的功能性分子鏈以及大環(huán)分子α-環(huán)糊精。具體工作如下:合成4-肼基甲基偶氮苯,在光控條件下研究其與0α-環(huán)糊精的自組裝行為。在制備的MCM-41表面修飾醛基三乙氧基硅烷和4-肼基甲基偶氮苯,反應(yīng)形成腙鍵,用α-環(huán)糊精將DOX藥物分子封裝在介孔中。采用透射電鏡等對改性的納米材料進(jìn)行表征。使用紫外-可見分光光度計(jì)研究MSNPs-2在酸性條件和紫外光照射下藥物釋放情況,驗(yàn)證了 MSNPs-2的pH和光雙響應(yīng)性能。MCF-7細(xì)胞實(shí)驗(yàn)證明載體細(xì)胞毒性較小,在紫外光照射條件下可在細(xì)胞內(nèi)快速釋放藥物,實(shí)現(xiàn)藥物在癌細(xì)胞環(huán)境中的有效釋放,在生物醫(yī)藥領(lǐng)域有潛在的應(yīng)用價(jià)值。
[Abstract]:The ideal drug carrier has biodegradable and controllable release properties in vivo. In this paper, the carrier decomposable material and multi-conditional drug controlled release system were prepared with silica nanomaterials as drug carrier. The study was divided into two parts: (1) an acid / alkali responsive silica / anticancer drug hybrid drug delivery system was introduced, which was obtained by the modified Stober method with the anticancer drug adriamycin (DOX) and silicon dioxide (SiO2). The structure of MSNPs-1 was determined by scanning transmission electron microscope (SEM). Transmission electron microscopy and UV-Vis spectrophotometer were used to study the drug release under different pH conditions. It was proved that the drug could be released in acidic or alkaline solution and the carrier could be decomposed at the same time. Cell experiments showed that MSNPs-1 could release drugs slowly in 7721 cells. To provide a theoretical basis for its application in the treatment of tumor in vivo, this paper introduces an intelligent nanovessel with both pH and UV response properties, which is prepared by modifying the surface of MCM-41 with Hydrazone structure and azobenzene. Functional molecular chains and macromolecular 偽-cyclodextrins of derivative recognition sites. The main work is as follows: 4-hydrazo-methylazobenzene was synthesized and its self-assembly behavior with 0 偽 -cyclodextrin was studied under light control. The surface of MCM-41 was modified with aldehyde triethoxy silane and 4-hydrazo-methylazobenzene to form Hydrazone bond. DOX was encapsulated in mesoporous cells by 偽 -cyclodextrin. The modified nanomaterials were characterized by transmission electron microscopy (TEM). UV-Vis spectrophotometer was used to study the drug release of MSNPs-2 under acidic and ultraviolet irradiation. The pH and photoresponse of MSNPs-2. MCF-7 cell experiment showed that the cytotoxicity of MSNPs-2 was less than that of MCF-7 cells. The drug can be released rapidly in cells under ultraviolet irradiation, which can effectively release the drug in the environment of cancer cells. It has potential application value in the field of biomedicine.
【學(xué)位授予單位】：南京理工大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R943

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