本文選題：腎陽(yáng)虛便秘 + 濟(jì)川煎��； 參考：《山西省中醫(yī)藥研究院》2017年碩士論文

【摘要】：目的運(yùn)用活性炭聯(lián)合冰水和5%乙酸法,制造大鼠腎陽(yáng)虛便秘動(dòng)物模型,探究溫陽(yáng)通便法治療腎陽(yáng)虛便秘與胃動(dòng)素(MTL)、P物質(zhì)(SP)和生長(zhǎng)抑素(SS)水平以及與cAMP、cGMP、T3、T4的關(guān)系。方法60只SD大鼠隨機(jī)分為5組,即空白組,模型組,濟(jì)川煎高、中、低劑量組,每組12只,把濟(jì)川煎高(3.8g/kg)、中(1.9g/kg)、低(0.95g/kg)劑量組,分別配制成25.2、12.6、6.3%的濃度,5%乙酸合并活性炭冰水造模4周后,各組按1.5ml/100g灌胃給藥,模型組給予等體積生理鹽水,每天1次,連續(xù)7天。觀察大鼠的一般生理變化,記錄大鼠首粒排便時(shí)間及其6小時(shí)內(nèi)排便數(shù)量,以及大鼠結(jié)腸的肌電和HE染色病理切片,分別用Elisa法和免疫組化法(SABC法)檢測(cè)大鼠血清和結(jié)腸中MTL、SP和SS水平等實(shí)驗(yàn),并用Elisa法測(cè)定大鼠血清cAMP、cGMP、T3、T4含量。結(jié)果結(jié)腸病理切片各實(shí)驗(yàn)組均未見差異。造模后大鼠一般生理學(xué)特征為:腹脹、披毛變硬、身體蜷縮、體重變輕、大便色黑干燥。與空白組對(duì)比,模型組的大鼠排便時(shí)間延長(zhǎng),排便粒數(shù)減少,胃腸蠕動(dòng)減弱,血清和結(jié)腸中MTL水平降低,P物質(zhì)不變,SS含量升高。治療組的上述體征和指標(biāo)有明顯改善,其中濟(jì)川煎高、中劑量組與濟(jì)川煎低劑量組比較,療效更明顯。此外還觀察到了濟(jì)川煎能夠明顯糾正腎陽(yáng)虛便秘模型動(dòng)物cAMP、cGMP失衡的作用,但本模型T3、T4無(wú)明顯變化。結(jié)論活性炭聯(lián)合冰水和5%乙酸法造模后,結(jié)腸沒(méi)有發(fā)生器質(zhì)性病變,屬于功能性便秘,大鼠各體征表現(xiàn)符合腎陽(yáng)虛證候,所造出的大鼠便秘模型是腎陽(yáng)虛便秘模型。濟(jì)川煎治療腎陽(yáng)虛便秘機(jī)理與提高M(jìn)TL水平,降低SS水平有密切關(guān)系,與P物質(zhì)關(guān)系不大,且濟(jì)川煎高、中劑量組治療腎陽(yáng)虛便秘效果更加顯著,濟(jì)川煎還通過(guò)糾正本模型動(dòng)物cAMP、cGMP的失衡而發(fā)揮改善腎陽(yáng)虛的功能,體現(xiàn)了標(biāo)本兼治的作用特點(diǎn)。
[Abstract]:Objective to establish an animal model of kidney-yang deficiency constipation in rats by using activated carbon combined with ice water and 5% acetic acid, and to explore the relationship between the treatment of kidney-yang deficiency constipation and motilin motilin (motilin) and somatostatin (SS), and the relationship between them and cAMPG MPT _ (3) T _ (3) T _ (4). Methods Sixty Sprague-Dawley rats were randomly divided into 5 groups: blank group, model group, Jichuan decoction high, middle and low dose groups, 12 rats in each group. The rats were divided into 3. 8 g / kg, 1. 9 g / kg, 0. 95 g / kg and 0. 95 g / kg, respectively. After 4 weeks of modeling with 5% acetic acid and activated carbon ice water, each group was given intragastric administration of 1.5ml/100g, and the model group was given normal saline of equal volume once a day for 7 days. To observe the general physiological changes of rats, to record the first defecation time and the number of defecation within 6 hours, and to observe the myoelectric and HE staining pathological sections of rat colon. The levels of MTLS-SP and SS in serum and colon of rats were detected by Elisa method and immunohistochemical method, respectively. The content of cAMP-cGMP-T3T3T4 in serum of rats was measured by Elisa method. Results there was no difference in colonic pathological section among the experimental groups. The general physiological characteristics of the model rats were abdominal distension, drape, body curling, light weight, dry stool color and black. Compared with the blank group, the defecation time of the model group was prolonged, the defecation granule number was decreased, the gastrointestinal peristalsis was weakened, the MTL level in serum and colon was decreased and the content of SS was increased. The above signs and indexes in the treatment group were obviously improved, among them, the effect of Jichuan decoction was higher than that of the middle dose group and the Jichuan decoction low dose group. In addition, it was observed that Jichuan decoction could obviously correct the imbalance of cAMPN cGMP in animal model of kidney yang deficiency constipation, but there was no obvious change of T3T4 in this model. Conclusion the model of activated carbon combined with ice water and 5% acetic acid has no organic lesion and belongs to functional constipation. The symptoms of each sign of rats accord with the syndrome of deficiency of kidney yang. The model of constipation in rats is the model of constipation due to deficiency of kidney yang. The mechanism of Jichuan decoction in treating constipation of kidney-yang deficiency was closely related to raising MTL level and decreasing SS level, but had little to do with substance P, and Jichuan decoction had a higher effect on constipation of kidney-yang deficiency, and the effect of medium dose group on constipation of kidney-yang deficiency was more remarkable. Jichuan decoction also improved the function of kidney yang deficiency by correcting the imbalance of cAMP and cGMP in the model animal, and reflected the function of both symptoms and symptoms.
【學(xué)位授予單位】：山西省中醫(yī)藥研究院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R256.35

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 譚從娥;黃祥云;郭小青;;應(yīng)用iTRAQ技術(shù)篩選右歸丸對(duì)腎陽(yáng)虛證干預(yù)效應(yīng)的血清差異表達(dá)蛋白[J];時(shí)珍國(guó)醫(yī)國(guó)藥;2014年09期

2 王秀鳳;張磊;張琰;唐小婭;羅來(lái)成;;基于支持向量機(jī)的腎陽(yáng)虛大鼠甲狀腺與腎上腺功能的動(dòng)態(tài)分析[J];時(shí)珍國(guó)醫(yī)國(guó)藥;2014年08期

3 劉醫(yī)輝;楊世英;馬偉林;宋易航;;當(dāng)歸藥理作用的研究進(jìn)展[J];中國(guó)當(dāng)代醫(yī)藥;2014年22期

4 田碩;苗明三;;牛膝的化學(xué)、藥理及應(yīng)用特點(diǎn)探討[J];中醫(yī)學(xué)報(bào);2014年08期

5 徐文聃;王欣;王琛;楊元宵;李守業(yè);李昌煜;;從下丘腦-垂體-腎上腺皮質(zhì)軸探討附子肉桂在腎氣丸中補(bǔ)腎陽(yáng)的作用[J];浙江中醫(yī)藥大學(xué)學(xué)報(bào);2014年07期

6 馬娜;羅來(lái)成;朱東海;;右歸丸對(duì)腎陽(yáng)虛大鼠垂體-腎上腺軸動(dòng)態(tài)影響的實(shí)驗(yàn)研究[J];中華中醫(yī)藥學(xué)刊;2014年06期

7 張宗保;;鹿茸益腎膠囊治療腎陽(yáng)虛證陽(yáng)痿的藥效學(xué)研究[J];中國(guó)執(zhí)業(yè)藥師;2014年06期

8 于曉娟;;功能性便秘的發(fā)病機(jī)制分析[J];藥物與人;2014年05期

9 高潔;王旭;;中醫(yī)補(bǔ)陽(yáng)法在治療甲減中應(yīng)用[J];遼寧中醫(yī)藥大學(xué)學(xué)報(bào);2014年03期

10 陳飛;陳卓;邢雪飛;劉素香;張鐵軍;陳常青;;肉蓯蓉的研究進(jìn)展[J];藥物評(píng)價(jià)研究;2013年06期

相關(guān)博士學(xué)位論文 前1條

1 顏亭祥;“勞倦過(guò)度、房事不節(jié)”腎陽(yáng)虛模型下丘腦—垂體—腎上腺皮質(zhì)—胸腺軸的改變及分子免疫機(jī)制的研究[D];山東中醫(yī)藥大學(xué);2010年

，

本文編號(hào)：1798143