本文選題：妊娠高血壓疾病 + 單核苷酸多態(tài)性�。� 參考：《青海大學》2017年碩士論文

【摘要】：目的通過研究內皮型一氧化氮合酶(endothelial nitricoxide synthase,e NOS)基因G894T、促紅細胞生成素(erythropoietin,EPO)基因T3541G、腫瘤壞死因子-α(tumour necrosises factor-alpha,TNF-α)基因G308A等多態(tài)性位點基因型頻率在青海省漢族妊娠高血壓疾病(pregnancy-induced hypertension syndrom,PIH)患者和正常孕婦之間分布的差異性,檢測兩組血漿中EPO、TNF-α含量,比較兩組血液中e NOS m RNA相對表達差異性,探討e NOS基因G894T、EPO基因T3541G、TNF-α基因G308A多態(tài)性與妊娠高血壓疾病的關聯(lián)性,為青海地區(qū)妊娠高血壓疾病的預防提供依據。方法收集青海省各大醫(yī)院漢族人群中妊娠高血壓患者和正常孕婦外周血標本和臨床資料,提取基因組DNA,采用限制性片段長度多態(tài)性聚合酶鏈反應(polymerase chain reaction-restriction fragment length polymorphism,PCR-RFLP)方法分別擴增e NOS SNP/G894T、EPO SNP/T3541G、TNF-αSNP/G308A等多態(tài)性位點,采用特異性酶對e NOS基因G894T、EPO基因T3541G、TNF-α基因G308A進行多態(tài)性分型。分別對不同基因型的目的片段測序,將測序結果在美國國立生物技術信息中心(national center for biotechnology information,NCBI)數(shù)據庫中比對,確保擴增產物真實可靠,并對酶切結果加以驗證;使用酶聯(lián)免疫吸附測定法(enzyme-linked immunosorbent assay,ELISA)檢測血漿中EPO、TNF-α含量;使用半定量反轉錄-聚合酶鏈反應(semi-quantitative reverse transcription and polymerase Chain reaction,Sq RT-PCR)方法分析血液中e NOS基因m RNA表達情況。結果1.e NOS基因GG、GT、TT基因型頻率在PIH組分別為17.4%、82.6%、0%,對照組分別為0%、95.6%、4.4%,兩組比較存在差異性(P0.05)。PIH組G、T等位基因頻率分別為58.7%、41.3%,對照組分別為47.8%、52.2%,兩組比較差異有統(tǒng)計學意義(P0.05),PIH組G等位基因頻率高于對照組。PIH組比對照組(OR=1.553,95%CI為1.107~2.178),G等位基因為PIH的易感基因(OR=1.229,95%CI為1.048~1.441)。2.EPO基因TT、TG、GG基因型頻率在PIH組分別為9.7%、74.2%、6.1%,對照組分別為42.9%、46.4%、10.7%,兩組比較有差異(P0.05)。PIH組野生純合型(TT)頻率明顯低于對照組,突變型(TG、GG)基因型頻率高于對照組。PIH組等位基因T、G頻率分別為46.8.%,53.2%,對照組分別為66.1%、33.9%,兩組比較差異有統(tǒng)計學意義(P0.05),PIH組T等位基因頻率低于對照組,G基因頻率明顯高于對照組,PIH比對照組(OR=0.451,95%CI為0.311~0.655),G等位基因為妊高征的危險因素(OR=1.569,95%CI為1.263~1.949)。3.TNF-α基因GG、GA、AA基因型頻率在PIH組中分別為18.18%、72.73%、9.09%,對照組為42.86%、52.38%、4.76%,兩組比較有顯著差異性(P0.01)。PIH組突變型(GA+AA)基因型頻率(81.82%)明顯高于對照組(57.14%)。PIH組G等位基因頻率(54.55%)低于對照組(69.05%),A等位基因頻率(45.45%)高于對照組(33.95%),(P0.01)。PIH組比對照組(OR=0.538,95%CI:0.375~0.771),A等位基因為PIH的易感基因(OR=1.469,95%CI:1.170~1.843)4.PIH組血漿中EPO含量為(454.113±35.097)pg/m L,對照組為(396.316±50.262)pg/m L,PIH組明顯高于對照組(P0.01)。5.TNF-α含量在PIH組血漿中為(21.976±17.570)pg/m L,對照組為(27.797±14.730)pg/m L兩組比較沒有統(tǒng)計學差異(P0.05)。6.PIH組e NOS m RNA表達相對強度為(1.007±0.155),對照組為(1.243±0.329),PIH組低于對照組(P0.05)。結論1.e NOS SNP/G894T、EPO SNP/T3541G、TNF-αSNP/G308A多態(tài)性與青海省漢族妊娠高血壓疾病相關,e NOS SNP/G894T位點G等位基因、EPO SNP/T3541G位點G等位基因、TNF-αSNP/G308A位點A等位基因為妊娠高血壓疾病的易感基因。2.EPO基因在妊娠高血壓疾病患者血漿中高表達。3.TNF-α含量在PIH患者血漿中沒有變化。4.e NOS m RNA在PIH患者血液中低表達。
[Abstract]:Objective to study the genotype frequencies of the polymorphic loci, such as endothelial nitricoxide synthase (E NOS) gene G894T, erythropoietin (erythropoietin, EPO) gene, and tumor necrosis factor - alpha (tumour necrosises factor-alpha, alpha) gene polymorphic loci. The difference in distribution between nancy-induced Hypertension Syndrom, PIH) and normal pregnant women, the content of EPO, TNF- alpha in two groups of plasma, and the relative expression of E NOS m RNA in the two groups, and the association between the e NOS gene, the polymorphism of the alpha gene and the pregnancy induced hypertension, and the pregnancy induced pregnancy in Qinghai region Methods to provide basis for the prevention of pregnancy induced hypertension. Methods to collect the peripheral blood samples and clinical data of pregnant hypertension and normal pregnant women from the Han population of Qinghai Province, and to extract genomic DNA and use the restrictive fragment length polymorphism polymerase chain reaction (polymerase chain reaction-restriction fragment length polymorphism, PCR). -RFLP) the polymorphic loci, such as e NOS SNP/G894T, EPO SNP/T3541G, TNF- alpha SNP/G308A, were amplified by the method, and the polymorphic typing was carried out by specific enzymes to e NOS gene G894T, EPO gene and alpha gene. For biotechnology information, NCBI) comparison in the database to ensure the authenticity of the products, and to verify the results of the enzyme digestion; using the enzyme linked immunosorbent assay (enzyme-linked immunosorbent assay, ELISA) to detect the content of EPO, TNF- a in plasma, and the use of Semidefinite reverse transcription polymerase chain reaction (Semi-quantitative reverse) Iption and polymerase Chain reaction, Sq RT-PCR) method analysis of E NOS gene m RNA expression in blood. Results 1.e genes were 17.4%, 82.6%, 0%, respectively 0%, 95.6%, 4.4% in the control group, and two groups were 58.7%, 41.3%, and control components, respectively. There were significant differences in the 47.8%, 52.2%, and two groups (P0.05). The frequency of G alleles in group PIH was higher than that in the control group (OR=1.553,95%CI was 1.107~2.178), and the G allele was PIH's susceptibility gene (OR=1.229,95%CI 1.048~1.441).2.EPO gene TT, and the frequency of genotype was 9.7%, 74.2%, 6.1%, and control component, respectively. The frequency of wild homozygous type (TT) in group.PIH was significantly lower than that of control group in group 42.9%, 46.4%, 10.7% and two. The frequency of mutant type (TG, GG) genotype was higher than that of.PIH group T in the control group, the frequency of G was 46.8.%, 53.2%, and the control group was 66.1%, 33.9% and two, respectively (P0.05), PIH group T allele frequency. The frequency of G gene was significantly higher than that in the control group, PIH was higher than the control group (OR=0.451,95%CI 0.311~0.655), G allele was the risk factor of pregnancy induced hypertension (OR=1.569,95%CI 1.263~1.949).3.TNF- a gene GG, GA, AA genotype frequencies were 18.18%, 72.73%, 9.09% respectively in the PIH group, 42.86%, 52.38%, 4.76% in the control group, and two groups were compared with the two groups. The genotype frequency of P0.01.PIH group (81.82%) was significantly higher than that of control group (57.14%).PIH group G allele frequency (54.55%) was lower than that of control group (69.05%), A allele frequency (45.45%) was higher than that of control group (33.95%), (P0.01).PIH group than control group (OR=0.538,95%CI:0.375~0.771), A allele was PIH susceptible gene (OR=1.4) 69,95%CI:1.170~1.843) the content of EPO in plasma of 4.PIH group was (454.113 + 35.097) pg/m L, the control group was (396.316 + 50.262) pg/m L, PIH group was significantly higher than that of control group (P0.01).5.TNF- alpha in PIH group plasma (21.976 + 17.570) pg/m, and the control group was (27.797 + 14.730). The relative intensity of the expression was (1.007 + 0.155), the control group was (1.243 + 0.329), and the PIH group was lower than the control group (P0.05). Conclusion 1.e NOS SNP/G894T, EPO SNP/T3541G, TNF- alpha SNP/G308A polymorphism is associated with pregnancy induced hypertension in Qinghai Han nationality, e NOS SNP/G894T locus allele. The high expression of.2.EPO gene in the plasma of hypertensive disorder complicating pregnancy with high expression of.3.TNF- alpha in the plasma of patients with pregnancy induced hypertension does not change the low expression of.4.e NOS m RNA in the blood of PIH patients.

【學位授予單位】：青海大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R714.246

【相似文獻】

相關期刊論文 前10條

1 米瑪;尼珍;;妊娠高血壓合并大量腹水一例[J];西藏科技;2009年09期

2 劉鳳華;;妊娠高血壓疾病死亡53例原因分析[J];中國社區(qū)醫(yī)師(醫(yī)學專業(yè)半月刊);2009年18期

3 高萍;;輕度妊娠高血壓患者心理健康狀況調查[J];航空航天醫(yī)藥;2010年01期

4 ;治療妊娠高血壓的新途徑[J];現(xiàn)代醫(yī)院;2010年10期

5 張祖蓮;;妊娠高血壓疾病的治療與預防[J];醫(yī)學信息(上旬刊);2011年08期

6 趙輝;;妊娠高血壓合并可逆性后部白質腦病綜合征12例護理體會[J];中國實用神經疾病雜志;2013年08期

7 蘇印權;;妊娠高血壓失調[J];黑龍江醫(yī)藥;1996年05期

8 李云霞;妊娠高血壓合并大量腹水1例報告[J];職業(yè)與健康;1998年06期

9 卞宜敏;;妊娠高血壓疾病的預測及預防效果觀察[J];中國保健營養(yǎng);2012年12期

10 陳妍華,馬云,鄭玉巧,劉鋒;妊娠高血壓產后血壓轉歸與圍孕產期干預關系探討[J];中國婦幼保健;2001年04期

相關會議論文 前10條

1 劉俊蘭;李紅燕;李梁;;早期發(fā)病型妊娠高血壓疾病的特點(附54例臨床分析)[A];全國圍產醫(yī)學專題學術研討會論文匯編[C];2007年

2 賈廣慧;;妊娠高血壓疾病的護理[A];河南省婦產科護理風險管理研討班暨學術會議論文集[C];2008年

3 楊孜;;妊娠高血壓疾病降壓相關臨床問題[A];中華醫(yī)學會第十次全國婦產科學術會議產科會場（產科學組、妊高癥學組）論文匯編[C];2012年

4 林其德;;妊娠高血壓疾病診治指南解讀[A];中華醫(yī)學會第三次全國妊娠期高血壓疾病學術研討會論文匯編[C];2011年

5 余艷紅;;妊娠高血壓疾病的抗凝治療[A];中華醫(yī)學會第三次全國妊娠期高血壓疾病學術研討會論文匯編[C];2011年

6 劉英華;李紅;李海波;毛君;劉敏娟;陳瑛;;妊娠高血壓及其相關并發(fā)癥的研究進展[A];中華醫(yī)學會第十次全國婦產科學術會議產科會場（產科學組、妊高癥學組）論文匯編[C];2012年

7 李笑天;;從轉化醫(yī)學視角分析妊娠高血壓疾病的研究策略[A];中華醫(yī)學會第三次全國妊娠期高血壓疾病學術研討會論文匯編[C];2011年

8 牛秀敏;李忷s，

本文編號：1796372