本文選題：壓力 + 動(dòng)脈粥樣硬化��； 參考：《廣州中醫(yī)藥大學(xué)》2017年碩士論文

【摘要】：目的:為了觀察壓力對(duì)小鼠血清炎癥反應(yīng)以及動(dòng)脈粥樣硬化斑塊的穩(wěn)定性的影響,從炎癥之角度探討壓力在動(dòng)脈粥樣硬化發(fā)生發(fā)展中所起的作用,并予越鞠丸進(jìn)行干預(yù),觀察中醫(yī)藥在壓力致動(dòng)脈粥樣硬化發(fā)生中的干預(yù)作用。方法:實(shí)驗(yàn)通過予以不同應(yīng)激刺激建立慢性應(yīng)激的動(dòng)物模型,將48只Apo E-/-小鼠隨機(jī)分為6組,空白組、模型組、越鞠丸低倍劑量+阿托伐他汀組、越鞠丸中倍劑量+阿托伐他汀組、越鞠丸高倍劑量+阿托伐他汀組、阿托伐他汀組,六組均予高脂飲食,除外空白組其余各組隨機(jī)安排應(yīng)激實(shí)驗(yàn)持續(xù)十二周,實(shí)驗(yàn)開始即給藥,十二周后采血查E選擇素、hs-CRP,取主動(dòng)脈應(yīng)用免疫組化方法分析神經(jīng)肽Y、E選擇素、脂蛋白磷脂酶A2在小鼠主動(dòng)脈中的表達(dá)差異并計(jì)算主動(dòng)脈內(nèi)斑塊的易損指數(shù)。另設(shè)一組取正常8只C57B1/6的雄性小鼠,正常飲食喂養(yǎng),不予應(yīng)激試驗(yàn),不予藥物治療。結(jié)果:予壓力刺激的小鼠血清中hs-CRP、E-選則素的含量增加,標(biāo)志著炎癥反應(yīng)的發(fā)生,予越鞠丸干預(yù)各組的主動(dòng)脈中E選擇素、神經(jīng)肽Y、脂蛋白磷脂酶A2的表達(dá)較模型組減少,E選擇素中可見越鞠丸的劑量效應(yīng)。同時(shí)以易損指數(shù)評(píng)估,炎癥反應(yīng)的發(fā)生與斑塊的穩(wěn)定性呈負(fù)相關(guān)。結(jié)論:壓力可以增加冠心病發(fā)病的風(fēng)險(xiǎn),是冠心病的危險(xiǎn)因素之一。壓力下的小鼠血清hsCRP、E-選則素的含量增加,表明慢性壓力應(yīng)激可導(dǎo)致炎癥反應(yīng)的發(fā)生;同時(shí)通過病理學(xué)檢測(cè)也證明,予慢性壓力應(yīng)激刺激后小鼠主動(dòng)脈中E選擇素、神經(jīng)肽Y、脂蛋白磷脂酶A2的表達(dá)增多,炎癥與斑塊的穩(wěn)定性呈負(fù)相關(guān);予越鞠丸干預(yù)后,炎癥標(biāo)志物的含量下降,提示越鞠丸可在一定程度上緩解斑塊炎癥從而達(dá)到穩(wěn)定斑塊的作用,為從郁論治冠心病提供了有力證據(jù)。
[Abstract]:Objective: to observe the effect of pressure on the inflammatory response in serum and the stability of atherosclerotic plaques in mice, to explore the role of pressure in the occurrence and development of atherosclerosis from the perspective of inflammation, and to intervene with Yueju pill.To observe the effect of traditional Chinese medicine (TCM) on pressure-induced atherosclerosis.Methods: 48 Apo E-r-mice were randomly divided into 6 groups, blank group, model group and low-dose Atto vastatin group.The middle dose Atto vastatin group, the high dose Atto vastatin group, the Atto vastatin group, the six groups were given high-fat diet, except the blank group, the other groups were randomly assigned to the stress test for 12 weeks. The drug was given immediately at the beginning of the experiment.After 12 weeks, blood samples were collected to examine the expression of E-selectin hs-CRP.The expression of neuropeptide Ya-E selectin and lipoprotein phospholipase A _ 2 in mouse aorta was analyzed by immunohistochemical method and the vulnerability index of plaques in aorta was calculated.Another group of 8 normal C57B1/6 male mice were fed with normal diet, no stress test, no drug treatment.Results: the content of hs-CRPnE- selectin in the blood serum of pressure-stimulated mice was increased, indicating the occurrence of inflammatory reaction. Yueju pill was given to interfere with E-selectin in aorta of each group.Compared with the model group, the expression of neuropeptide Y, lipoprotein phospholipase A2 decreased the dose-effect of Yueju pill.At the same time, the occurrence of inflammation and plaque stability were negatively correlated with vulnerability index.Conclusion: stress can increase the risk of coronary heart disease and is one of the risk factors of coronary heart disease.The increase of serum hsCRP- E- selectin in mice under stress suggests that chronic stress can lead to inflammatory reaction, and that E selectin in aorta of mice stimulated by chronic stress is also proved by pathological examination.The expression of neuropeptide Y, lipoprotein phospholipase A2 was increased, and inflammation was negatively correlated with plaque stability.The results suggest that Yueju pill can alleviate plaque inflammation and stabilize plaque to a certain extent, which provides strong evidence for treating coronary heart disease from depression.
【學(xué)位授予單位】：廣州中醫(yī)藥大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R285.5

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