本文選題：杜仲葉　切入點(diǎn)：化學(xué)成分　出處：《新疆醫(yī)科大學(xué)》2017年碩士論文

【摘要】：目的:研究并比較不同產(chǎn)地杜仲葉藥材的品質(zhì)、四種化學(xué)成分的定性定量分析、微量元素的含量測定,為杜仲葉藥材的品質(zhì)進(jìn)行評價(jià)。方法:根據(jù)2015版《中華人民共和國藥典》和查閱大量文獻(xiàn)對杜仲葉藥材進(jìn)行性狀鑒定;對杜仲葉藥材粉末進(jìn)行顯微鑒別;對7批杜仲葉藥材中化學(xué)成分綠原酸進(jìn)行薄層鑒別;使用紫外分光光度計(jì)測定7批杜仲葉提取液中的總多糖、總黃酮、總多酚含量;采用傅里葉變換紅外光譜法(FTIR)對杜仲葉藥材進(jìn)行紅外光譜的研究,快速建立杜仲葉藥材紅外指紋圖譜,并對7批杜仲葉進(jìn)行聚類分析;利用高效液相色譜法(HPLC)測定7批杜仲葉藥材中綠原酸、槲皮素、山奈酚和蘆丁的含量;采用火焰原子吸收分光光度法測定7批杜仲葉藥材、土壤樣品、灌溉水樣品中鉀、鈉、鈣、鎂、鐵、鋅、錳7種金屬元素的含量,采用石墨爐原子吸收法測定土壤樣品中鉛的含量和7批杜仲葉藥材、土壤樣品、灌溉水樣中銅的含量。結(jié)果:薄層色譜鑒別最優(yōu)固定相選擇青島海洋H高效板,以乙酸丁酯-甲酸-水(7:2.5:2.5)的上層溶液為展開劑,7批杜仲葉薄層色譜中所含成分基本一致;7批杜仲葉藥材提取液中總多糖含量在16.02-37.87 mg/g之間、總黃酮含量在30.97-119.07 mg/g之間、總多酚含量在9.89-36.74 mg/g之間,7批杜仲葉藥材總成分的含量均有所差異,其中,樣品S7的總多糖、總黃酮、總多酚含量均為較高;從杜仲葉藥材的紅外光譜峰形、峰位等信息可知,7批杜仲葉光譜特征基本一致,在通過二階導(dǎo)數(shù)變換后,使得7批藥材紅外光譜之間的差異放大,通過聚類分析對杜仲葉進(jìn)行分類,可將杜仲葉藥材分為5組;7批杜仲葉藥材中綠原酸、槲皮素、山奈酚、蘆丁的含量有所差異,其中綠原酸含量在0.8353-3.3624 mg/g之間、槲皮素含量在0.1608-0.6129 mg/g之間、山奈酚含量在0.0236-0.1031 mg/g之間、蘆丁含量在0.0022-0.0043 mg/g之間;7批杜仲葉藥材中鉀、鈉、鈣、鎂、鐵、鋅、錳、銅的含量分別在15300.95-40300.95、58.24-298.98、3319.15-9515.96、8335.17-12138.92、211.40-933.63、21.92-72.62、144.79-560.05、209.21-406.58μg/g之間,土壤樣品中鉀、鈉、鈣、鎂、鐵、鋅、錳、銅、鉛的含量平均含量為43310.19、678.89、9015.96、511.03、22836.26、118.53、656.90、47.93、21.69μg/g之間,灌溉水樣品中鉀、鈉、鈣、鎂、鐵、鋅、錳、銅的含量平均含量為534.72、7065.13、2840.43、2086.00、38.71、0.13、0.186、18.16μg/mL之間,其中鉀、鈣等元素含量普遍較高,重金屬元素檢查均符合國家標(biāo)準(zhǔn)。結(jié)論:本研究初步探索并比較新疆產(chǎn)杜仲葉和其他4個(gè)產(chǎn)地杜仲葉中總多糖、總黃酮、總多酚含量;對7批藥材進(jìn)行了性狀鑒定、顯微鑒別、薄層鑒別;采用HPLC法測定7批杜仲葉藥材中綠原酸、槲皮素、山奈酚、蘆丁的含量;測定了杜仲葉藥材、土壤樣品、灌溉水樣品中微量元素的含量。為建立科學(xué)的杜仲葉品質(zhì)標(biāo)準(zhǔn)提供科學(xué)依據(jù),使新疆產(chǎn)杜仲葉藥用資源得到廣泛的開發(fā)和利用。
[Abstract]:Objective: to study and compare the quality of Eucommia ulmoides leaves from different areas, the qualitative and quantitative analysis of four chemical constituents, and the determination of trace elements. Methods: according to the 2015 edition of the Pharmacopoeia of the people's Republic of China and consulting a large number of literature to identify the character of Eucommia ulmoides leaves, to identify the powder of Eucommia ulmoides leaves by microscope, to evaluate the quality of Eucommia ulmoides L. The content of total polysaccharides, total flavonoids and total polyphenols in 7 batches of Eucommia ulmoides leaves extract was determined by UV spectrophotometer. Fourier transform infrared spectroscopy (FTIR) was used to study the infrared spectrum of Eucommia ulmoides leaves. The infrared fingerprint of Eucommia ulmoides leaves was established quickly, and 7 batches of Eucommia ulmoides leaves were analyzed by cluster analysis. The contents of Lv Yuan, quercetin, kaempferol and rutin in 7 batches of Eucommia ulmoides leaves were determined by HPLC, and potassium and sodium in 7 batches of Eucommia ulmoides leaves, soil samples and irrigation water samples were determined by flame atomic absorption spectrophotometry. The contents of calcium, magnesium, iron, zinc and manganese in soil samples were determined by graphite furnace atomic absorption spectrometry (GFAAS). The content of Copper in Irrigation Water samples. Results: TLC was used to identify the optimal stationary phase in Qingdao Ocean H High efficiency Plate. The contents of total polysaccharides and flavonoids were between 16.02-37.87 mg/g and 30.97-119.07 mg/g in TLC of 7 batches of Eucommia ulmoides leaves by TLC with butyl acetate-formic acid-water 7: 2.5: 2.5) as the developing agent, and the contents of total polysaccharides in the extracts of Eucommia ulmoides ulmoides were between 16.02-37.87 mg/g and 30.97-119.07 mg/g, respectively. The contents of total polyphenols in the leaves of Eucommia ulmoides were all different between 9.89-36.74 mg/g, and the contents of total polysaccharides, total flavonoids and total polyphenols of sample S7 were higher. The spectral characteristics of Eucommia ulmoides leaves in 7 batches of Eucommia ulmoides were basically the same. After the second derivative transformation, the infrared spectra of 7 batches of Eucommia ulmoides were amplified, and the leaves of Eucommia ulmoides were classified by cluster analysis. The contents of Lv Yuan, quercetin, kaempferol and rutin in the leaves of Eucommia ulmoides ulmoides could be divided into 5 groups. The contents of Lv Yuan acid, quercetin and kaempferol were 0.8353-3.3624 mg/g, 0.1608-0.6129 mg/g and 0.0236-0.1031 mg/g, respectively. The contents of potassium, sodium, calcium, calcium, magnesium, iron, zinc, manganese and copper in seven batches of Eucommia ulmoides leaves were between 0.0022 and 0.0043 mg/g. The average contents of potassium, sodium, calcium, magnesium, iron, manganese, copper and copper were 15300.95-40300.95n 58.24-298.98.93319.15-9515.915.919.15-9515.95.12138.911.40-933.6321.92-72.6144.569-0.05209.21-406.58 渭 g / g of potassium, sodium, calcium, magnesium, iron, zinc, manganese, copper and lead, respectively. The average contents of potassium, sodium, calcium, magnesium, iron, zinc, manganese and copper in the irrigation water samples were 534.72C 7065.135.132840.43n 2086.0038.71C 0.130.186n 18.16 渭 g/mL, among which the contents of potassium, calcium and other elements were generally higher. Conclusion: the contents of total polysaccharides, total flavonoids and total polyphenols in leaves of Eucommia ulmoides from Xinjiang and other four areas were preliminarily explored and compared. The content of Lv Yuan, quercetin, kaempferol and rutin in 7 batches of Eucommia ulmoides leaves were determined by HPLC. The content of trace elements in irrigation water samples provides a scientific basis for establishing scientific quality standards of Eucommia ulmoides leaves and makes the medicinal resources of Eucommia ulmoides leaves widely developed and utilized in Xinjiang.
【學(xué)位授予單位】：新疆醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R284.1

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