本文選題：精子DNA損傷　切入點：精子凋亡　出處：《大連醫(yī)科大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：目的:通過對89例精液常規(guī)、精子凋亡率及精子DNA碎片化指數(shù)的檢測,探討腎陽虛、腎陰虛型男性不育癥患者與其他不育癥患者之間精液常規(guī)、精子凋亡率、精子DNA碎片化指數(shù)的差異,以及男性不育癥患者精子凋亡率與精子DNA碎片化指數(shù)之間的相關(guān)性。方法:按照納入標(biāo)準(zhǔn)分別收集腎陽虛組(n=30)、腎陰虛組(n=30)及對照組(n=29)共89例男性不育患者精液標(biāo)本,分別使用計算機輔助精液分析技術(shù)(computer-assisted semen analysis,CASA)檢測各組精液常規(guī)參數(shù),流式細胞術(shù)(Flow Cytomentry,FCM)結(jié)合吖啶橙染色法(acridine orange,AO),檢測精子凋亡率(sperm apoptosis)及精子DNA碎片化指數(shù)(sperm DNA Fragmentation Index,DFI)。統(tǒng)計使用SPSS20.0統(tǒng)計軟件對已收集的數(shù)據(jù)進行統(tǒng)計學(xué)分析,計量資料以均數(shù)±標(biāo)準(zhǔn)差(X±S)表示。組間比較采用兩獨立樣本的t檢驗。精子凋亡率與DFI相關(guān)性根據(jù)數(shù)據(jù)的正態(tài)分布或偏態(tài)分布,采用Pearson相關(guān)性分析進行統(tǒng)計學(xué)分析。P0.05認(rèn)為差異有統(tǒng)計學(xué)意義,P0.01為差異有明顯的統(tǒng)計學(xué)意義。結(jié)果:1.腎陽虛組、腎陰虛組在年齡分布與對照組相比較P0.05(P=0.581),無統(tǒng)計學(xué)差異;腎陽虛組、腎陰虛組與對照組在病史時間上相比較P0.05(P=0.404),無統(tǒng)計學(xué)差異。2.腎陰虛組、腎陽虛組精液量、精子密度與對照組相比較,P0.05(腎陰虛組P=0.823,腎陽虛組P=0.419)無統(tǒng)計學(xué)差異;腎陰虛組的a級精子活力百分比、a+b級精子活力百分比低于對照組,P0.05(a級精子百分比P=0.013,a+b級精子百分比P=0.042)具有統(tǒng)計學(xué)差異,精子活動率高于對照組,P0.05(P=0.181)無統(tǒng)計學(xué)差異;腎陽虛組的a級精子活力百分比、a+b級精子活力百分比、精子活動率全部低于對照組,其中a級精子活力百分比有顯著的統(tǒng)計學(xué)差異P0.01(a級精子百分比P=0.000),a+b級精子百分比、精子活動率有統(tǒng)計學(xué)差異P0.05(a+b級精子百分比P=0.050,精子活動率P=0.047)。3.腎陰虛組精子凋亡率高于對照組,P0.05(P=0.028)有統(tǒng)計學(xué)差異,DNA碎片化指數(shù)高于對照組,P0.01(P=0.000)有顯著統(tǒng)計學(xué)差異;腎陽虛組精子凋亡率、精子DFI指數(shù)均高于對照組,P0.01(精子凋亡率P=0.010,精子DFI指數(shù)P=0.000)差異有顯著統(tǒng)計學(xué)意義。4.全部受試對象的精子凋亡率與DFI結(jié)果經(jīng)過Spearman相關(guān)性分析,計算相關(guān)系數(shù)得r:0.285,P=0.007,具有顯著的統(tǒng)計學(xué)差異。結(jié)論:1.腎陽虛、腎陰虛型不育癥患者精子活力、精子活率均低于其他男性不育癥患者,DNA碎片化程度、精子凋亡率均高于其他男性不育癥患者,提示腎陽虛、腎陰虛型男性不育癥患者與其他不育癥患者相比精子活力及生育力更低。2.男性不育癥患者精子DNA碎片率與精子凋亡率呈顯著正相關(guān)線性關(guān)系,提示精子DNA損傷可能與精子細胞的凋亡有關(guān)。
[Abstract]:Objective: to investigate the semen routine, sperm apoptosis rate and sperm apoptosis rate between male infertility patients and other infertility patients with deficiency of kidney yang and kidney yin deficiency by detecting sperm routine, sperm apoptosis rate and DNA fragmentation index in 89 cases of male infertility with kidney yang deficiency and kidney yin deficiency. The difference of sperm DNA fragmentation index, And the correlation between sperm apoptosis rate and sperm DNA fragmentation index in male infertile patients. Methods: according to the inclusive criteria, the semen samples of 89 male infertile patients were collected according to the inclusive criteria, respectively, in the kidney-yang deficiency group, the kidney-yin deficiency group, and the control group. The semen parameters of each group were detected by computer-assisted semen analysis (CASA). Flow Cytomentry (FCM) and acridine orange staining (Acridine orange staining) were used to detect sperm apoptosis rate and sperm DNA fragmentation index (Sperm DNA Fragmentation index). The collected data were statistically analyzed by SPSS20.0 software. The measurement data were expressed as mean 鹵standard deviation X 鹵S. T test of two independent samples was used for the comparison between the two groups. The correlation between sperm apoptosis rate and DFI was based on the normal distribution or skewness distribution of the data. The Pearson correlation analysis showed that the difference was statistically significant (P0.01). Results: 1. There was no significant difference in age distribution between kidney-yang deficiency group and kidney-yin deficiency group compared with control group (P 0.05), but there was no significant difference between kidney yang deficiency group, kidney yang deficiency group and control group. There was no statistical difference between the kidney yin deficiency group, the kidney yang deficiency group and the control group in the time of the disease history. 2. There was no statistical difference between the kidney yin deficiency group, the kidney yang deficiency group and the control group in the quantity of spermatozoa and the sperm density (P 0. 05) (P 0. 823 in the kidney yin deficiency group, P 0. 419 in the kidney yang deficiency group). The percentage of spermatozoa motility in the group of kidney yin deficiency was lower than that in the control group (P < 0.05). There was no significant difference in the percentage of spermatozoa activity between the two groups. There was no significant difference in the percentage of spermatozoa activity between the two groups (P _ (0.05) P _ (0.181)) and the percentage of sperm motility was higher than that of the control group (P _ (0.05) P _ (0.181)). The percentage of spermatozoa motility of grade a and the percentage of sperm motility of grade a were all lower than those of the control group, among which there was significant difference between the percentage of sperm motility of grade a and the percentage of spermatozoa of grade a, among them, the percentage of spermatozoa of grade a was significantly different from that of the control group (P0. 01). There was significant difference in sperm motility rate (P0.05A / b), percentage of spermatozoa (P < 0.050) and sperm motility (P _ (0.047) 路3.The rate of sperm apoptosis in the group of kidney yin deficiency was higher than that in the control group (P _ (0.05) P _ (0.028)). The fragmentation index of DNA was significantly higher than that of the control group (P _ (0.01) P _ (0.000)). The sperm apoptosis rate and sperm DFI index were significantly higher in the kidney-yang deficiency group than in the control group (P0. 010, P0. 010, P0. 000). There was a significant difference between the sperm apoptosis rate and the DFI results in all subjects by Spearman correlation analysis. The correlation coefficient was r: 0.285 and P = 0.007, with significant statistical difference. Conclusion: 1. The sperm motility and sperm motility rate of infertility patients with deficiency of kidney yang and yin deficiency of kidney are lower than those of other male infertility patients, and the degree of DNA fragmentation is lower than that of other male infertility patients. The rate of spermatozoa apoptosis was higher than that of other male infertility patients, suggesting deficiency of kidney yang, The sperm motility and fertility of male infertility patients with kidney yin deficiency type were lower than those of other infertility patients. 2. There was a significant positive correlation between sperm DNA fragment rate and sperm apoptosis rate in male infertile patients. These results suggest that DNA damage may be related to the apoptosis of sperm cells.
【學(xué)位授予單位】：大連醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R698.2
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本文編號：1601545